Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antimicrobial Activity of Flos Syzygii Aromatici Extracts against Mice Infected with Escherichia coli O157:H7

Escherichia coli O157:H7에 감염된 마우스에 대한 정향 추출물의 항균효과

  • Lee, Soo-Mi (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Son, Song Ee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu Jang (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University)
  • 이수미 (경상대학교 보건대학원 환경보건학과) ;
  • 손송이 (경상대학교 수의과대학.동물의학연구소) ;
  • 이후장 (경상대학교 보건대학원 환경보건학과)
  • Received : 2017.05.19
  • Accepted : 2017.07.09
  • Published : 2017.08.30
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Abstract

The present study evaluated the antibacterial effect of Flos syzygii Aromatici methanolic extracts (FSAE). In addition, the effectiveness of FSAE against Escherichia coli O157:H7 infection was studied using ICR female mice. At 24 h after incubation of E. coli O157:H7, FSAE at the concentration of 0.269 (p < 0.05), 0.538 (p < 0.001) and 1.075 mg/mL (p < 0.001) significantly inhibited the growth of E. coli O157:H7 compared to the control group. After single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were orally administered with saline (control), 0.538 (group 1), 1.075 (group 2) and 2.15 mg/mL (group 3) of FSAE, respectively. On the 3rd day, the number of fecal E. coli O157:H7 in group 2 (p < 0.05) and group 3 (p < 0.01) was significantly decreased compared to that in the control group. On the 7th day post-treatment, the number of fecal E. coli O157:H7 in all FSAE-treated groups was significantly decreased compared to that in the control group (group 1, p < 0.05; group 2 and 3, p < 0.001). According to the results of the present study, administration of FSAE to mice can reduce the severity of E. coli O157:H7 infection. Therefore, the current study suggests that FSAE could be a good candidate for the treatment of enteric infections in domestic animals.

본 연구는 정향추출물의 E. coli O157:H7에 대한 항균효과와 E. coli O157:H7 감염 마우스에 대한 치료효과를 평가하기 위해 수행되었다. 정향 메탄올 추출물(FSAE)을 이용하여 E. coli O157:H7에 대한 항균효과 확인 시험을 수행한 결과, 배양 후 24시간째에, FSAE를 첨가한 모든 군들에서 E. coli O157:H7의 생존율이 무투여 대조군에 비해 통계적으로 유의성 있게 감소하는 결과를 보여(0.269 mg/ml, p < 0.05; 0.538과 1.075 mg/ml, p < 0.001), FSAE가 E. coli O157:H7의 증식억제 효과가 뛰어난 것으로 확인되었다. 또한, E. coli O157:H7을 감염시킨 마우스에 FSAE를 경구로 투여한 결과, 투여 후 3일째에, FSAE를 1.075 (p < 0.05)와 2.15 mg/ml (p < 0.01)로 투여한 군들에서 대조군과 비교하여 분변내 E. coli O157:H7의 균수가 유의성 있게 감소하였으며, 투여 7일째에는, 모든 FSAE 투여군들에서 대조군과 비교하여 E. coli O157:H7의 균수가 통계적으로 유의성 있게 감소하였다(0.538 mg/ml, p < 0.05; 1.075와 2.15 mg/ml, p < 0.001). 이상의 연구결과로부터, FSAE를 E. coli O157:H7에 감염된 마우스에 경구로 투여할 경우, 감염증상을 완화 시킬 수 있을 것으로 기대된다.

Keywords

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