Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지
DOI QR코드

DOI QR Code

A Pilot Study of Relationship Between Early Childhood Caries Experience and Chair-side Test Results for Caries-Risk Assessment

우식위험도 검사 결과와 유아기 우식증 사이의 상관관계에 대한 예비연구

  • Heo, Seon-Jae (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Shin, Teo-Jeon (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Hyun, Hong-Keun (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Jung-Wook (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Jang, Ki-Taeg (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Lee, Sang-Hoon (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Young-Jae (Department of Pediatric Dentistry, School of Dentistry, Seoul National University)
  • 허선재 (서울대학교 치의학대학원 소아치과학교실) ;
  • 신터전 (서울대학교 치의학대학원 소아치과학교실) ;
  • 현홍근 (서울대학교 치의학대학원 소아치과학교실) ;
  • 김정욱 (서울대학교 치의학대학원 소아치과학교실) ;
  • 장기택 (서울대학교 치의학대학원 소아치과학교실) ;
  • 이상훈 (서울대학교 치의학대학원 소아치과학교실) ;
  • 김영재 (서울대학교 치의학대학원 소아치과학교실)
  • Received : 2016.10.06
  • Accepted : 2016.11.22
  • Published : 2017.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to compare chair-side test results for caries risk assessment and evaluate how well the tests reflect caries experience. The study was conducted on children aged < 6 years in primary dentition. Dental examination of children was conducted to determine the dmft index and subjects were divided into two groups : group I (dmft < 6), group II (dmft > 6). This study used four kinds of test kits (Plaque-check PH kit, Saliva-check buffer kit, Saliva-check mutans kit, Cytoperio analysis system). Saliva buffer capacity was significantly low in the high caries experience group (dmft > 6) and correlated with dmft index. Saliva pH level correlated significantly with saliva buffer capacity. The results showed that plaque pH and saliva pH levels had no correlation with dmft index. The Streptococcus mutans level measured by using the Saliva-check mutans and Cytoperio analysis system did not correlate with dmft index.

본 연구는 임상에서 사용되는 진단도구들이 소아에서 우식위험도와 상관관계를 가지는지 여부를 확인하고, 소아에서 우식위험도를 효과적으로 반영하는 지표가 무엇인지 알아보기 위함이다. 32명의 6세 미만의 소아를 두 군(I군 : dmft < 6, II군 : dmft > 6)으로 나누었다. Plaque-check PH을 이용해 치태의 pH를 측정하고, Saliva-check buffer를 이용해 타액의 pH와 완충능력를 측정하였다. S. mutans 검출을 위해 Saliva-check mutans와 Cytoperio analysis system을 이용하였다. 타액 완충능력은 두 군에서 유의한 차이를 보이며 dmft와 상관관계를 보였고, 타액의 pH와도 유의한 상관관계가 있는 것으로 확인되었다. 두 군 사이에 치태 pH, 타액 pH의 유의한 차이는 관찰되지 않으며 dmft와 상관관계를 가지지 않는 것으로 밝혀졌다. S. mutans의 단일클론항체를 이용하는 Saliva-check mutans와 real-time PCR에 기반한 Cytoperio analysis system은 dmft와 유의한 상관관계를 가지고 있지 않는 것으로 밝혀졌다.

Keywords

References

  1. American Academy of Pediatric dentistry : Policy on Early childhood caries (ECC): Classifications, consequences, and preventive strategies. Pediatr Dent, 37:50-52, 2014.
  2. Kang GM, LEE HS, Lee JH, et al. : The distribution of patients and treatment trends in the department of pediatric dentistry, Yonsei University Dental Hospital for last 5 years. J Korean Acad Pediatr Dent, 41:134-143, 2014. https://doi.org/10.5933/JKAPD.2014.41.2.134
  3. 2015 Korean children's oral health survey. Available from URL: http://www.mohw.go.kr/front_new/jb/sjb030301vw.jsp?PAR_MENU_ID=03&MENU_ID=0321&CONT_SEQ=332448&page=1 (Accessed on July 17, 2016).
  4. Son YJ, Hyun HK, Jang KT, et al. : The changes in practice patterns for the last 8 years(2001-2008) in the department of pediatric dentistry, Seoul National University Dental Hospital. J Korean Acad Pediatr Dent, 37:97-100, 2010.
  5. Korean Academy of Pediatric Dentist : Textbook of Pediatric Dentistry, 5th ed., Dental Wisdom, 274, 2014.
  6. Kim YJ : New vision in pediatric dentistry - Keeping healthy teeth caries free : Pediatric CAMBRA protocols. J Korean Acad Pediatr Dent, 40:72-81, 2013. https://doi.org/10.5933/JKAPD.2013.40.1.72
  7. Li Y, Wang W : Predicting caries in permanent teeth from caries in primary teeth: an eight-year cohort study. J Dent Res, 81:561-6, 2002. https://doi.org/10.1177/154405910208100812
  8. Pawell LV : Caries prediction: a review of the literature. Community Dent Oral Epidemiol, 26:361-371, 1998. https://doi.org/10.1111/j.1600-0528.1998.tb01974.x
  9. Dong YM, Pearce EI, Wang JD, et al. : Plaque pH and associated parameters in relation to caries. Caries Res, 33:428-436, 1999. https://doi.org/10.1159/000016547
  10. Fejerskov O, Scheie AA, Manji F : The effect of sucrose on plaque pH in the primary and permanent dentition of caries-inactive and -active Kenyan children. J Dent Res, 71:25-31, 1992. https://doi.org/10.1177/00220345920710010401
  11. Sansone C, Van Houte J, Margolis HC, et al. : The association of mutans streptococci and non-mutans streptococci capable of acidogenesis at a low pH with dental caries on enamel and root surfaces. J Dent Res, 72:508-516, 1993. https://doi.org/10.1177/00220345930720020701
  12. Van Houte J, Lopman J, Kent R : The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces. J Dent Res, 75:1008-1014, 1996. https://doi.org/10.1177/00220345960750040201
  13. Humphrey SP, Williamson RT : A review of saliva: normal composition, flow, and function. J Prosthet Dent, 85:162-169, 2001. https://doi.org/10.1067/mpr.2001.113778
  14. Anderson P, Hector MP, Rampersad MA : Critical pH in resting and stimulated whole saliva in groups of children and adults. Int J Paediatr Dent, 11:266-273, 2011.
  15. Singh S, Sharma A, Sinha A, et al. : Saliva as a prediction tool for dental caries : An in vivo study. J Oral Biol Craniofac Res, 5:59-64, 2015. https://doi.org/10.1016/j.jobcr.2015.05.001
  16. Prabhakar A, Dodawad R, Os R : Evaluation of flow rate, pH, buffering capacity, calcium, total protein and total antioxidant levels of saliva in caries free and caries active children-An in vivo study. Int J Clin Pediatr Dent, 2:8-12, 2009.
  17. Marsh PD : Are dental diseases examples of ecological catastrophes? Microbiology, 149:279-294, 2003. https://doi.org/10.1099/mic.0.26082-0
  18. Twetman L, Twetman S : Comparison of two chair-side tests for enumeration of Mutans Streptococci in saliva. Oral Health Dent Manag, 13:580-583, 2014.
  19. Gao XL, Seneviratne CJ, Samaranayake LP, et al. : Novel and conventional assays in determining abundance of Streptococcus mutans is saliva. Int J Paediatr Dent, 22:363-368, 2012. https://doi.org/10.1111/j.1365-263X.2011.01207.x
  20. Lee MJ, Lee DW, Kim JG, et al. : Detection of Streptococcus mutans in saliva using monoclonal antibodies. J Korean Acad Pediatr Dent, 42:10-21, 2015. https://doi.org/10.5933/JKAPD.2015.42.1.10
  21. Rupf S, Merte K, Eschrich K, et al. : Comparison of different techniques of quantitative PCR for determination of Streptococcus mutans counts in saliva samples. Oral Microbiol Immunol, 18:50-53, 2003. https://doi.org/10.1034/j.1399-302X.2003.180108.x
  22. Igarashi T, Yamamoto A, Goto N : Direct detection of Streptococcus mutans in human dental plaque by polymerase chain reaction. Oral Microbiol Immunol, 5:294-298, 1996.
  23. Yano A, Kaneko N, Hanada N, et al. : Real-time PCR for quantification of Streptococcus mutans. FEMS Microbiol Lett, 217:23-30, 2002. https://doi.org/10.1111/j.1574-6968.2002.tb11451.x
  24. Salazar O : Bacteria and Yeast cell disruption using lytic enzymes. Methods Mol Biol, 424:23-34, 2008.
  25. Bollet C, Gevaudan MJ, de Lamballerie X, et al. : A sample method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria. Nucleic Acids Res, 19:1955, 1991. https://doi.org/10.1093/nar/19.8.1955
  26. Reich E, Lussi A, Newbrun E : Caries-risk assessment. Int Dent J, 49:15-26, 1999. https://doi.org/10.1111/j.1875-595X.1999.tb00503.x
  27. Demers M, Brodeur JM, Mouton C, et al. : A multivariate model to predict caries increment in Montreal children aged 5 years. Comm Dnt Health, 9:273-281, 1992.

Cited by

  1. Validity of the New Caries Activity Test using Real-Time Polymerase Chain Reaction vol.45, pp.3, 2018, https://doi.org/10.5933/JKAPD.2018.45.3.354