Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Evaluation on Antimicrobial Activity of Psoraleae semen Extract Controlling the Growth of Gram-Positive Bacteria

  • Kim, Sejeong (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Lee, Soomin (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Lee, Heeyoung (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Ha, Jimyeong (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Lee, Jeeyeon (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Choi, Yukyung (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Oh, Hyemin (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Hong, Joonbae (Test & Research Department, Consumer Safety Center, Korea Consumer Agency) ;
  • Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Choi, Kyoung-Hee (Department of Oral Microbiology, College of Dentistry, Wonkwang University)
  • Received : 2017.02.08
  • Accepted : 2017.07.04
  • Published : 2017.08.31
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Abstract

This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were $8{\mu}g/mL$ for Streptococcus mutans, and $16{\mu}g/mL$ for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration $8-16{\mu}g/mL$. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.

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References

  1. Bereksi, N., Gavini, F., Benezech, T., and Faille, C. (2002) Growth, morphology and surface properties of Listeria monocytogenes Scott A and LO28 under saline and acid environments. J. Appl. Microbiol. 92, 556-565. https://doi.org/10.1046/j.1365-2672.2002.01564.x
  2. Fani, M. M., Kohanteb, J., and Dayaghi, M. (2007) Inhibitory activity of garlic (Allum sativum) extract on multidrug-resistant Streptococcus mutans. J. Indian Soc. Pedod. Prev. Dent. 25, 164-168. https://doi.org/10.4103/0970-4388.37011
  3. Guliani, A., Pirri, G., and Nicoletto, S. F. (2007) Antimicrobial peptides: An overview of a promising class of therapeutics. Cent. Eur. J. Biol. 2, 1-33.
  4. Henie, E. F. P., Zaiton, H., and Suhaila, M. (2009) Bacterial membrane disruption in food pathogens by Psidium guajava leaf extracts. Int. Food Res. J. 16, 297-311.
  5. Hood, S. K. and Zottola E. A. (1995) Bioflms in food processing. Food Control 6, 9-18. https://doi.org/10.1016/0956-7135(95)91449-U
  6. Hwang, J. H., Rha, S. J., Cho, J. K., Shin, T. S., Kim, M. Y., and Kim, J. D. (2013) Psoraleae semen extract inhibits angiogenesis and adipogenesis. Biotechnol. Bioproc. Eng. 18, 942-949. https://doi.org/10.1007/s12257-013-0379-0
  7. Jackson, A. L., Kulchaiyawat, C., Sullivan, G. A., Sebranek, J. G., and Dickson, J. S. (2011) Use of natural ingredients to control growth of Clostridium perfringens in naturally cured frankfurters and hams. J. Food. Prot. 74, 417-424. https://doi.org/10.4315/0362-028X.JFP-10-365
  8. Katsura, H., Tsukiyama, R. I., Suzuki, A., and Kobayashi, M. (2001) In vitro antimicrobial activities of bakuchiol against oral microorganisms. Antimicrob. Agents Chemother. 45, 3009-3013. https://doi.org/10.1128/AAC.45.11.3009-3013.2001
  9. Kaya, I., Yigit, N., and Benli, M. (2008) Antimicrobial activity of various extracts of Ocimum Basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Aft. J. Trad. CAM. 5, 363-369.
  10. Kuete, V., Wabo, G. F., Ngameni, B., Mbaveng, A. T., Metuno, R., Etoa, F. X., Ngadjui, B. T., Beng, V. P., Meyer, J. J. M., and Lall, N. (2007) Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvinia gabonensis (Ixonanthaceae). J. Ethnopharmacol. 114, 54-60. https://doi.org/10.1016/j.jep.2007.07.025
  11. Leela, T. and Satirapipathkul, C. (2011) Studies on the antibacterial activity of quercus infectoria galls. Int. Con. Biosci. Biochem. Bioinform. (IPCBEE) 5, 410-414.
  12. Liu, C. S., Cham, T. M., Yan, C. H., Chang, H. W., Chen, C. H., and Chuan, L. Y. (2007) Antibacterial properties of Chinese herbal medicines against nosocomial antibiotic resistant strains of Pseudomonas aeruginosa in Taiwan. Am. J. Chinese Med. 35, 1047-1060. https://doi.org/10.1142/S0192415X07005508
  13. Loesche, W. J. (1986) Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353-380.
  14. Loo, C. Y., Corliss, D. A., and Ganeshkumar, N. (2000) Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes. J. Bacteriol. 182, 1374-1382. https://doi.org/10.1128/JB.182.5.1374-1382.2000
  15. Navarre, W. W. and Schneewind, O. (1999) Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol. Mol. Biol. Rev. 63, 174-229.
  16. NCCLS (National Committee for Clinical Llaboratory Standards). (1997) Performance standards for antimicrobial disk susceptibility tests: Approved standard M2-A7. Wayne: PA: USA.
  17. Plesiat, P. and Nikaido, H. (1992) Outer membranes of gramnegative bacteria are permeable to steroid probes. Mol. Microbiol. 6, 1323-1333. https://doi.org/10.1111/j.1365-2958.1992.tb00853.x
  18. Shim, S. H., Kim, Y., Lee, J. Y., and Song, D. G. (2009) Aldose reductase inhibitory activity of the compounds from the seed of Psoralea corylifolia. J. Korean Soc. Appl. Biol. Chem. 52, 568-572. https://doi.org/10.3839/jksabc.2009.096
  19. Wong, R. W. K. and Rabiem, A. B. M. (2010) Effect of Buguzhi (Psoralea corylifolia fruit) extract on bone formation. Phytother. Res. 24, 155-160. https://doi.org/10.1002/ptr.3049
  20. Wu, F., Wang, A. F., Zhou, Y., Zhou, T. S., Jiang, H. L., and Fang, Y. Z. (2005) Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection. Chromatographia 61, 157-160. https://doi.org/10.1365/s10337-004-0476-5
  21. Xiong, Z., Wang, D., Wu, Y., and Li, F. (2003) Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L. Int. J. Phamaceutic. Sci. 58, 925-928.
  22. Yim, N. H., Jung, Y. P., Cho, W. K, Kim, T., Kim, A., Im, M., and Ma, J. Y. (2013) Screening of aqueous extracts of medicinal herbs for antimicrobial activity against oral bacteria. J. Integr. Med. 2, 18-24.
  23. Yoon, Y. and Choi, K. H. (2010) Identification of inhibitory effect on Streptococcus mutans by oleanolic acid. J. Life Sci. 3, 321-325.
  24. Yoon, Y. and Choi, K. H. (2012) Antimicrobial activities of therapeutic herbal plants against Listeria monocytogenes and the herbal plant cytotoxicity on Caco-2 cell. Lett. Appl. Microbiol. 55, 47-55. https://doi.org/10.1111/j.1472-765X.2012.03262.x
  25. Zaide, S. F., Yamada, K., Kadowaki, M., Usmanghani, K., and Sugiyama, T. (2009) Bactericidal activity of medicinal plants, employed for the treatment of gastrointestinal ailments, against Helicobacter pylori. J. Ethnopharmacol. 121, 286-291. https://doi.org/10.1016/j.jep.2008.11.001

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