Gut and Liver

The Korean Association for the Study of the Liver (대한간학회)
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Heme Oxygenase-1 Induction and Anti-inflammatory Actions of Atractylodes macrocephala and Taraxacum herba Extracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

  • Han, Kyu-Hyun (Digestive Disease Center, CHA University Bundang Medical Center) ;
  • Park, Jong-Min (CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University) ;
  • Jeong, Migyeong (CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University) ;
  • Han, Young-Min (CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University) ;
  • Go, Eun-Jin (CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University) ;
  • Park, Juyeon (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.) ;
  • Kim, Hocheol (Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Han, Jae Gab (Department of Health Food Research & Development, Daesang Corp.) ;
  • Kwon, Oran (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Hahm, Ki Baik (Digestive Disease Center, CHA University Bundang Medical Center)
  • Received : 2016.09.27
  • Accepted : 2017.01.09
  • Published : 2017.09.15
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Abstract

Background/Aims: In inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of Atractylodes macrocephala (AM) or Taraxacum herba (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration. Methods: Murine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge. Acute colitis was induced with 7 days of dextran sulfate sodium (DSS) in male C57BL/6 mice, and extracts of AM and TH were administered for 2 weeks before DSS administration. Results: In vitro studies demonstrated that AM or TH treatment reduced LPS-induced COX-2 and tumor necrosis $factor-{\alpha}$ mRNA levels but increased heme oxygenase-1 (HO-1). Oral preadministration of AM and TH rescued mice from DSS-induced colitis by inhibiting inflammatory mediators via inactivated extracellular signal regulated kinase and repressed nuclear factor ${\kappa}B$ and signal transducer and activator of transcription 3, but the effect was weaker for sulfasalazine than that for the extracts. Anti-inflammatory activities occurred via the inhibition of macrophage and T lymphocyte infiltrations. Unlike sulfasalazine, which did not induce HO-1, TH extracts afforded significant HO-1 induction. Conclusions: Because the AM or TH extracts were far superior in preventing DSS-induced colitis than sulfasalazine, AM or TH extracts can be considered natural agents that can prevent IBD relapse.

Keywords

Acknowledgement

Supported by : National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders (NCEED)

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