Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Protective effect on neuronal cells of Orostachys japonicus A. Berger extract against reactive oxygen species-induced neuronal cytotoxicity and active compounds

활성 산소종으로 야기된 산화스트레스에 대한 와송 추출물의 신경세포 보호효과 및 주요 생리활성물질

  • Park, Su Bin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Du Sang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jeong Eun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kwon, Bong Seok (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Chang Jun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 박수빈 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 이두상 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 강진용 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 강정은 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 권봉석 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 박상현 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 이창준 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원) ;
  • 이욱 (국립산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus), 농업생명과학연구원)
  • Received : 2017.05.01
  • Accepted : 2017.06.13
  • Published : 2017.10.31
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Abstract

The study aimed to investigate the antioxidant activity and neuroprotective effect of the ethyl acetate fraction from Orostachys japonicus A. Berger extract (EFOJ) and its main constituent compounds. Among all fractions, the highest content of total phenolics was found in EFOJ. The antioxidant activity of EFOJ was confirmed through the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1-1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP) assays and the inhibitory effect of malondialdehyde (MDA). In addition, we ascertained that EFOJ not only decreased the intracellular ROS level, but also protected the neuronal cells against $H_2O_2$-induced oxidative stress. In liquid chromatography-mass spectrometry analysis, the following were found to be the main compounds of EFOJ: quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rhamnoside. Consequently, these results suggested that the protective effect on neuronal cells was based on the antioxidant activities of the physiologically active compounds of Orostachys japonicus A. Berger extract, which could therefore help to mitigate neurodegenerative diseases.

본 연구에서는 와송(Orostachys japonicus A. Berger)의 산화방지 효과와 산화스트레스로부터의 신경세포 손상에 대한 보호효과 및 주요 생리활성물질을 분석하였다. 와송 아세트산에틸 분획물(ethylacetate fraction from Orostachys japonicus A. Berger extract: EFOJ)은 다른 분획물보다 높은 총 페놀화합물 함량을 나타냈으며, 와송 아세트산에틸 분획물(EFOJ)로 ABTS, DPPH 라디칼 소거 활성과 FRAP 검정 및 지방질과산화물 억제력 분석을 실시한 결과, 우수한 라디칼 소거 활성, 총산화방지력과 과산화 지방질 생성물의 억제력을 확인하였다. 또한 와송 아세트산에틸 분획물(EFOJ)은 과산화수소로 인한 신경세포 사멸에 대한 세포 생존율을 향상시켰으며, 신경 세포막 손상을 보호하는 능력이 우수한 것을 확인하였다. 이러한 생리 활성을 가진 와송 아세트산에틸 분획물(EFOJ)의 주요물질을 분석하기 위하여 $LC-MS^e$를 실시하였으며, 주요 생리활성 물질은 퀘세틴 배당체와 캠페롤 배당체로 추정되었다. 본 연구 결과들을 바탕으로, 와송 추출물은 우수한 산화방지력을 가지고 있을 뿐만 아니라 산화적 스트레스로부터의 신경세포 보호효과를 통해 퇴행성 신경질환과 같은 질병을 예방 할 수 있는 건강기능성식품의 천연산화방지제 소재로써 고부가 가치가 있다고 판단된다.

Keywords

References

  1. Fiers W, Beyaert R, Declercq W, Vandenabeele P. More than one way to die: Apoptosis and necrosis and reactive oxygen damage. Oncogene 18: 7719-7730 (1999) https://doi.org/10.1038/sj.onc.1203249
  2. Benzi G, Moretti A. Age-and peroxidative stress-related modifications of the cerebral enzymatic activities linked to mitochondria and the glutathione system. Free Radic. Biol. Med. 19: 77-101 (1995) https://doi.org/10.1016/0891-5849(94)00244-E
  3. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39: 44-84 (2007) https://doi.org/10.1016/j.biocel.2006.07.001
  4. Limon-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 674: 137-147 (2009) https://doi.org/10.1016/j.mrgentox.2008.09.015
  5. Roy MK, Juneja LR, Isobe S, Tsushida T. Steam processed broccoli (Brassica oleracea) has higher antioxidant activity in chemical and cellular assay systems. Food Chem. 114: 263-269 (2009) https://doi.org/10.1016/j.foodchem.2008.09.050
  6. Jin DH, Kim HS, Seong JH, Chung HS. Comparison of total phenol, flavonoid contents, and antioxidant activities of Orostachys japonicus A. Berger extracts. J. Environ. Sci. Int. 25: 695-703 (2016) https://doi.org/10.5322/JESI.2016.25.5.695
  7. Choi SY, Kim JG, Sung NJ. Studies on the physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger. J. Korean Soc. Food Sci. Nutr. 21: 148-156 (2008)
  8. Lee HS, Ryu DS, Lee GS, Lee DS. Anti-inflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: Suppression of NF-B activation and MAPK signaling. J. Ethnopharmacol. 140: 271-276 (2012) https://doi.org/10.1016/j.jep.2012.01.016
  9. Lee SJ, Shin JH, Ju JC, Kang SK, Sung NJ. Hypoglycemic and hypolipidemic effects of Orostachys japonicus with medicinal herbs in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 42: 587-594 (2013) https://doi.org/10.3746/jkfn.2013.42.4.587
  10. Kim JM, Park SK, Guo TJ, Kang JY, Ha JS, Lee DS, Kwon OJ, Lee U, Heo HJ. Neuronal cell protective effect of Dendropanax morbifera extract against high glucose-induced oxidative stress. J. Korean Soc. Food Sci. Nutr. 45: 938-947 (2016) https://doi.org/10.3746/jkfn.2016.45.7.938
  11. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agr. Food Chem. 49: 3420-3424 (2001) https://doi.org/10.1021/jf0100907
  12. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  13. Szabo MR, Idioiu C, Chambre D, Lupea AX. Improved DPPH determination for antioxidant activity spectrophotometric assay. Chem. Pap. 61: 214-216 (2007)
  14. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem. 239: 70-76 (1996) https://doi.org/10.1006/abio.1996.0292
  15. Lovell MA, Xie C, Markesbery WR. Acrolein is increased in Alzheimer' disease brain and is toxic to primary hippocampal cultures. Neurobiol. Aging 22: 187-194 (2001) https://doi.org/10.1016/S0197-4580(00)00235-9
  16. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr. Biol. 24: 453-462 (2014) https://doi.org/10.1016/j.cub.2014.03.034
  17. Heo HJ, Cho HY, Hong BS, Kim HK, Kim EK, Kim BG, Shin DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against A-induced oxidative stress in PC12 cells. Amyloid 8: 194-201 (2001) https://doi.org/10.3109/13506120109007362
  18. Zhao B. Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638 (2009) https://doi.org/10.1007/s11064-008-9900-9
  19. Berridge MV, Tan AS. Characterization of the Cellular Reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 303: 474-482 (1993) https://doi.org/10.1006/abbi.1993.1311
  20. Korzeniewski C, Callewaert DM. An enzyme-release assay for natural cytotoxicity. J. Immunol. Methods 64: 313-320 (1983) https://doi.org/10.1016/0022-1759(83)90438-6
  21. Felipe DF, Brambilla LZS, Porto C, Pilau EJ, Cortez DAG. Phytochemical analysis of Pfaffia glomerata inflorescences by LCESI-MS/MS. Molecules 19: 15720-15734 (2014) https://doi.org/10.3390/molecules191015720
  22. Zhang L, Zhu L, Wanga Y, Jiang Z, Chai X, Zhu Y, Gao X, Qi A. Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD-MS/MS. J. Pharm. Biomed. Anal. 62: 203-209 (2012) https://doi.org/10.1016/j.jpba.2011.12.026
  23. Farag MA, Sakna ST, El-fiky NM, Shabana MM, Wessjohann LA. Antioxidant and antidiabetic evaluation of eight Bauhinia L. species from Egypt using UHPLC-PDA--qTOF-MS and chemometrics. Phytochem. 119: 41-50 (2015) https://doi.org/10.1016/j.phytochem.2015.09.004
  24. Liao SG, Zhang LJ, Sun F, Wang Z, He X, Wang AM, Li YZ, Huang Y, Lan YY, Zhang BL, Wang YL. Identification and characterisation of phenolics in Polygonum capitatum by ultrahighperformance liquid chromatography with photodiode array detection and tandem mass spectrometry. Phytochem. Anal. 24: 556-568 (2013) https://doi.org/10.1002/pca.2432
  25. Lee JH, Lee SJ, Park SM, Kim HK, Jeong WY, Choi JY, Sung NJ, Lee WS, Lim CS, Kim GS, Shin SC. Characterisation of flavonoids in Orostachys japonicus A. Berger using HPLC-MS/MS: Contribution to the overall antioxidant effect. Food Chem. 124: 1627-1633 (2011) https://doi.org/10.1016/j.foodchem.2010.08.031