The Journal of Advanced Prosthodontics

The Korean Academy of Prosthodonitics (대한치과보철학회)
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Adherence of Candida to complete denture surfaces in vitro: A comparison of conventional and CAD/CAM complete dentures

  • Al-Fouzan, Afnan F. (Prosthetic Dental Science, College of Dentistry, King Saud University) ;
  • Al-mejrad, Lamya A. (Prosthetic Dental Science, College of Dentistry, King Saud University) ;
  • Albarrag, Ahmed M. (Department of Pathology, School of Medicine, King Saud University)
  • Received : 2017.02.07
  • Accepted : 2017.07.04
  • Published : 2017.10.31
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Abstract

PURPOSE. The goal of this study was to compare the adhesion of Candida albicans to the surfaces of CAD/CAM and conventionally fabricated complete denture bases. MATERIALS AND METHODS. Twenty discs of acrylic resin poly (methyl methacrylate) were fabricated with CAD/CAM and conventional procedures (heat-polymerized acrylic resin). The specimens were divided into two groups: 10 discs were fabricated using the CAD/CAM procedure (Wieland Digital Denture Ivoclar Vivadent), and 10 discs were fabricated using a conventional flasking and pressure-pack technique. Candida colonization was performed on all the specimens using four Candida albicans isolates. The difference in Candida albicans adhesion on the discs was evaluated. The number of adherent yeast cells was calculated by the colony-forming units (CFU) and by Fluorescence microscopy. RESULTS. There was a significant difference in the adhesion of Candida albicans to the complete denture bases created with CAD/CAM and the adhesion to those created with the conventional procedure. The CAD/CAM denture bases exhibited less adhesion of Candida albicans than did the denture bases created with the conventional procedure (P<.05). CONCLUSION. The CAD/CAM procedure for fabricating complete dentures showed promising potential for reducing the adherence of Candida to the denture base surface. Clinical Implications. Complete dentures made with the CAD/CAM procedure might decrease the incidence of denture stomatitis compared with conventional dentures.

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References

  1. Cunha-Cruz J, Hujoel PP, Nadanovsky P. Secular trends in socio-economic disparities in edentulism: USA, 1972-2001. J Dent Res 2007;86:131-6. https://doi.org/10.1177/154405910708600205
  2. Emami E, de Souza RF, Kabawat M, Feine JS. The impact of edentulism on oral and general health. Int J Dent 2013;2013: 498305.
  3. Dye BA, Li X, Thorton-Evans G. Oral health disparities as determined by selected healthy people 2020 oral health objectives for the United States, 2009-2010. NCHS Data Brief 2012;(104):1-8.
  4. Douglass CW, Shih A, Ostry L. Will there be a need for complete dentures in the United States in 2020? J Prosthet Dent 2002;87:5-8. https://doi.org/10.1067/mpr.2002.121203
  5. Gotfredsen K, Walls AW. What dentition assures oral function? Clin Oral Implants Res 2007;18:34-45.
  6. Martori E, Ayuso-Montero R, Martinez-Gomis J, Vinas M, Peraire M. Risk factors for denture-related oral mucosal lesions in a geriatric population. J Prosthet Dent 2014;111:273-9. https://doi.org/10.1016/j.prosdent.2013.07.015
  7. de Oliveira CE, Gasparoto TH, Dionisio TJ, Porto VC, Vieira NA, Santos CF, Lara VS. Candida albicans and denture stomatitis: evaluation of its presence in the lesion, prosthesis, and blood. Int J Prosthodont 2010;23:158-9.
  8. Shulman JD, Rivera-Hidalgo F, Beach MM. Risk factors associated with denture stomatitis in the United States. J Oral Pathol Med 2005;34:340-6. https://doi.org/10.1111/j.1600-0714.2005.00287.x
  9. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont 2011;20:251-60. https://doi.org/10.1111/j.1532-849X.2011.00698.x
  10. Sadig W. The denture hygiene, denture stomatitis and role of dental hygienist. Int J Dent Hyg 2010;8:227-31.
  11. Yigit N, Aktas E, Dagistan S, Ayyildiz A. Investigating biofilm production, coagulase and hemolytic activity in Candida species isolated from denture stomatitis patients. Eurasian J Med 2011;43:27-32. https://doi.org/10.5152/eajm.2011.06
  12. Coco BJ, Bagg J, Cross LJ, Jose A, Cross J, Ramage G. Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. Oral Microbiol Immunol 2008;23:377-83. https://doi.org/10.1111/j.1399-302X.2008.00439.x
  13. Ramage G, VandeWalle K, Lopez-Ribot JL, Wickes BL. The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development in Candida albicans. FEMS Microbiol Lett 2002;214:95-100. https://doi.org/10.1111/j.1574-6968.2002.tb11330.x
  14. Mizugai H, Isogai E, Hirose K, Chiba I. Effect of denture wearing on occurrence of Candida species in the oral cavity. J Appl Res 2007;7:250-4.
  15. Samaranayake LP. Oral mycoses in HIV infection. Oral Surg Oral Med Oral Pathol 1992;73:171-80. https://doi.org/10.1016/0030-4220(92)90191-R
  16. Quirynen M, Marechal M, Busscher HJ, Weerkamp AH, Darius PL, van Steenberghe D. The influence of surface free energy and surface roughness on early plaque formation. An in vivo study in man. J Clin Periodontol 1990;17:138-44. https://doi.org/10.1111/j.1600-051X.1990.tb01077.x
  17. Sumi Y, Nagaosa S, Michiwaki Y, Sunakawa M, Miura H. Comparative study of denture and pharyngeal bacterial flora of dependent elderly. Jpn J Gerodont 2001;16:171-8.
  18. Kumamoto CA, Vinces MD. Alternative Candida albicans lifestyles: growth on surfaces. Annu Rev Microbiol 2005;59: 113-33. https://doi.org/10.1146/annurev.micro.59.030804.121034
  19. Ramage G, Tomsett K, Wickes BL, Lopez-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;98:53-9. https://doi.org/10.1016/j.tripleo.2003.04.002
  20. Spratt DA, Pratten J. Biofilms and the oral cavity. Rev Environ Sci Biotechnol 2003;2:109-20. https://doi.org/10.1023/B:RESB.0000040466.82937.df
  21. Ramage G, Martinez JP, Lopez-Ribot JL. Candida biofilms on implanted biomaterials: a clinically significant problem. FEMS Yeast Res 2006;6:979-86. https://doi.org/10.1111/j.1567-1364.2006.00117.x
  22. Chandra J, Mukherjee PK, Leidich SD, Faddoul FF, Hoyer LL, Douglas LJ, Ghannoum MA. Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J Dent Res 2001;80:903-8. https://doi.org/10.1177/00220345010800031101
  23. Kumar CP, Menon T. Biofilm production by clinical isolates of Candida species. Med Mycol 2006;44:99-101. https://doi.org/10.1080/13693780500338084
  24. Ramage G, Vande Walle K, Wickes BL, Lopez-Ribot JL. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 2001;45:2475-9. https://doi.org/10.1128/AAC.45.9.2475-2479.2001
  25. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26:577-83. https://doi.org/10.1016/S0300-5712(97)00034-1
  26. Hazen KC. Participation of yeast cell surface hydrophobicity in adherence of Candida albicans to human epithelial cells. Infect Immun 1989;57:1894-900.
  27. von Fraunhofer JA, Loewy ZG. Factors involved in microbial colonization of oral prostheses. Gen Dent 2009;57:136-43; quiz 144-5.
  28. Bidra AS, Taylor TD, Agar JR. Computer-aided technology for fabricating complete dentures: systematic review of historical background, current status, and future perspectives. J Prosthet Dent 2013;109:361-6. https://doi.org/10.1016/S0022-3913(13)60318-2
  29. Singh S, Palaskar JN, Mittal S. Comparative evaluation of surface porosities in conventional heat polymerized acrylic resin cured by water bath and microwave energy with microwavable acrylic resin cured by microwave energy. Contemp Clin Dent 2013;4:147-51. https://doi.org/10.4103/0976-237X.114844
  30. Maeda Y, Minoura M, Tsutsumi S, Okada M, Nokubi T. A CAD/CAM system for removable denture. Part I: Fabrication of complete dentures. Int J Prosthodont 1994;7:17-21.
  31. Kattadiyil MT, Goodacre CJ, Baba NZ. CAD/CAM complete dentures: a review of two commercial fabrication systems. J Calif Dent Assoc 2013;41:407-16.
  32. Steinmassl PA, Wiedemair V, Huck C, Klaunzer F, Steinmassl O, Grunert I, Dumfahrt H. Do CAD/CAM dentures really release less monomer than conventional dentures? Clin Oral Investig 2017;21:1697-705. https://doi.org/10.1007/s00784-016-1961-6
  33. Bollen CM, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater 1997;13:258-69. https://doi.org/10.1016/S0109-5641(97)80038-3
  34. Silva S, Henriques M, Martins A, Oliveira R, Williams D, Azeredo J. Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. Med Mycol 2009;47:681-9. https://doi.org/10.3109/13693780802549594
  35. Budtz-Jorgensen E. Etiology, pathogenesis, therapy, and prophylaxis of oral yeast infections. Acta Odontol Scand 1990; 48:61-9. https://doi.org/10.3109/00016359009012735
  36. Chandra J, Kuhn DM, Mukherjee PK, Hoyer LL, McCormick T, Ghannoum MA. Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resis-tance. J Bacteriol 2001;183:5385-94. https://doi.org/10.1128/JB.183.18.5385-5394.2001
  37. Chaffin WL, Lopez-Ribot JL, Casanova M, Gozalbo D, Martinez JP. Cell wall and secreted proteins of Candida albicans: identification, function, and expression. Microbiol Mol Biol Rev 1998;62:130-80.
  38. Final report of the workshop on clinical requirements of ideal denture base materials. The Academy of Denture Prosthetics. J Prosthet Dent 1968;20:101-5. https://doi.org/10.1016/0022-3913(68)90132-7
  39. Revised American Dental Association specification no. 12 for denture base polymers. J Am Dent Assoc 1975;90:451-8. https://doi.org/10.14219/jada.archive.1975.0069
  40. Wolfaardt JF, Cleaton-Jones P, Fatti P. The occurrence of porosity in a heat-cured poly (methyl methacrylate) denture base resin. J Prosthet Dent 1986;55:393-400. https://doi.org/10.1016/0022-3913(86)90128-9
  41. Keller JC, Lautenschlager EP. Porosity reduction and its associated effect on the diametral tensile strength of activated acrylic resins. J Prosthet Dent 1985;53:374-9. https://doi.org/10.1016/0022-3913(85)90516-5
  42. al Doori D, Huggett R, Bates JF, Brooks SC. A comparison of denture base acrylic resins polymerised by microwave irradiation and by conventional water bath curing systems. Dent Mater 1988;4:25-32. https://doi.org/10.1016/S0109-5641(88)80084-8
  43. Compagnoni MA, Barbosa DB, de Souza RF, Pero AC. The effect of polymerization cycles on porosity of microwaveprocessed denture base resin. J Prosthet Dent 2004;91:281-5. https://doi.org/10.1016/j.prosdent.2004.01.006
  44. Campbell SD. Evaluation of surface roughness and polishing techniques for new ceramic materials. J Prosthet Dent 1989; 61:563-8. https://doi.org/10.1016/0022-3913(89)90276-X
  45. Jung M. Finishing and polishing of a hybrid composite and a heat-pressed glass ceramic. Oper Dent 2002;27:175-83.
  46. Whitehead SA, Shearer AC, Watts DC, Wilson NH. Comparison of two stylus methods for measuring surface texture. Dent Mater 1999;15:79-86. https://doi.org/10.1016/S0109-5641(99)00017-2
  47. Kukiattrakoon B, Hengtrakool C, Kedjarune-Leggat U. Effect of acidic agents on surface roughness of dental ceramics. Dent Res J (Isfahan) 2011;8:6-15.
  48. Yazici AR, Muftu A, Kugel G. Three-dimensional surface profile analysis of different types of flowable restorative resins following different finishing protocols. J Contemp Dent Pract 2007;8:9-17.
  49. Joniot SB, Gregoire GL, Auther AM, Roques YM. Threedimensional optical profilometry analysis of surface states obtained after finishing sequences for three composite resins. Oper Dent 2000;25:311-5.
  50. Kasina SP, Ajaz T, Attili S, Surapaneni H, Cherukuri M, Srinath HP. To evaluate and compare the porosities in the acrylic mandibular denture bases processed by two different polymerization techniques, using two different brands of commercially available denture base resins - an in vitro study. J Int Oral Health 2014;6:72-7.

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