Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Gene Expression of Chironomus riparius Heat Shock Protein 70 and Developmental Retardation Exposure to Salinity

염분 노출에 따른 Chironomus riparius의 성장지연과 Heat shock protein 70 유전자 발현

  • Kim, Won-Seok (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Im, Byeong-Hyeon (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Hong, Cheol (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Choi, Seung-Won (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Park, Kiyun (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Kwak, Ihn-Sil (Department of Fisheries and Ocean Science, Chonnam National University)
  • 김원석 (전남대학교 해양기술학부) ;
  • 임병현 (전남대학교 해양기술학부) ;
  • 홍철 (전남대학교 해양기술학부) ;
  • 최승원 (전남대학교 해양기술학부) ;
  • 박기연 (전남대학교 해양기술학부) ;
  • 곽인실 (전남대학교 해양기술학부)
  • Received : 2017.05.30
  • Accepted : 2017.09.10
  • Published : 2017.09.30
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Abstract

We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on.

기후변화로 잦아진 강우에 의해 염분 환경에 유입되는 담수생물의 영향을 파악해 보고자 하였다. 담수의 오염지표생물인 깔따구과의 실내 실험종인 C. riparius를 대상으로 염분 노출에 따른 스트레스 반응으로 생존율과 탈피율, 성장기간, 하순기절 기형을 분석하였고, 분자생물학적으로 스트레스 분자마커로 연구가 이루어진 HSP70 유전자 발현을 측정하였다. C. riparius의 생존율은 노출시간에 따라 감소하였으며 15 psu에서 4일이 경과하면 생존개체가 관찰되지 않았다. C. riparius의 pupation과 emergence는 15 psu 이상에서는 보이지 않았고 탈피의 성공은 10 psu에서 20% 이하였다. 또한 C. riparius의 성장지연은 pupation과 emergence 기간에서 잘 반영되어, 대조군과 5 psu에 비해 10 psu에서 4일이 늦어졌다. 염분 노출 96시간 후 관찰한 C. riparius 하순기절 형태 이상은 10 psu와 15 psu에서 소수 관찰되었다. C. riparius HSP70 유전자 발현은 대조군에 비해 5, 10 psu에서 약 2.5~4배 이상 발현량이 크게 증가하였다. 따라서 염분은 담수생물에게 매우 큰 스트레스를 제공하는 원인물질로 유해물질과 유사한 생태독성학적인 반응과 분자지표 반응을 보여주었다.

Keywords

References

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