The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Inhibition of gene associated with Dyslipidemia and Antioxidative Effect of Artemisia iwayomogi, Curcumae Radix and Raphani Semen(ACR) on HepG2 cell model

HepG2 cell을 이용한 한인진, 울금, 나복자 복합물(ACR)의 이상지질혈증 관련 유전자 발현 억제 및 항산화 효능 평가

  • Cha, Jiyun (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Yoo, Ho-ryong (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Kim, Yoon-sik (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Seol, In-chan (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Jo, Hyun-kyung (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
  • 차지윤 (대전대학교 한의과대학 심계내과학교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실) ;
  • 조현경 (대전대학교 한의과대학 심계내과학교실)
  • Received : 2017.08.07
  • Accepted : 2017.09.12
  • Published : 2017.09.30
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Abstract

Objectives: We performed this study to evaluate the antioxidative and hypolipidemic effect of Artemisia iwayomogi (韓茵蔯), Curcuma longa L. (鬱金) and Raphanus sativus L. (蘿?子) (ACR). Method: We enriched Artemisiae Capillaris, Curcumae Longae and Raphani Semen compound with alcohol. ACR extract is treated to HepG2 cell. Cell groups are devided into 3 groups: normal, control and ACR treated group. We measured polyphenol, flavonoids, DPPH and ABTS radical scavenging activity, ROS, glutathione, GSH peroxidase, GSH reductase, SOD, catalase, free fatty acid, lipid peroxidation and suppression of ACAT1 and HMG-CoA reductase expression on mRNA level. Results: 1. ACR contained polyphenol and flavonoids and increased GSH significantly in HepG2 cell. 2. ACR increased GPx, GR, and catalase activity significantly in HepG2 cell. 3. ACR increased DPPH and ABTS radical scavenging activity significantly in HepG2 cell and decreased ROS. 4. ACR decreased free fatty acid and MDA significantly in HepG2 cell. 5. ACR suppressed ACAT1 and HMG-CoA reductase expression on mRNA level in HepG2 cell. Conclusion: This study suggests that ACR has antioxidative and hypolipidemic effect and might be effective in prevention and treatment of dyslipidemia.

Keywords

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