The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effects of Chrysanthemi Indici Flos Extract and Flaxseed Oil Mixture on HCl/ethanol-induced Acute Gastric Lesion Mice

급성 위염 동물 모델에서 감국(甘菊) 추출물과 아마인유(亞麻仁油) 혼합물의 위 점막 보호 효과

  • Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Ahn, Jeong-Hyun ;
  • Park, Hae-Jin (Department of Food Science and Nutrition, Daegu Hanny University) ;
  • Lee, Woo Rak ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 안정현 ((주)농부플러스) ;
  • 박해진 (대구한의대학교 한방식품영양조리학부) ;
  • 이우락 ((주)농부플러스) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2018.09.13
  • Accepted : 2018.11.25
  • Published : 2018.11.30
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Abstract

Objectives : The objective of this study was to investigate the protective effect of Flaxseed oil and Chrysanthemi Indici Flos 50% ethanol extract in an HCl/ethanol induced acute gastritis model. Methods : ICR mice were divided into 6 groups; normal mice (Nor), gastritic mice with distilled water (Veh), gastritic mice with 10 mg/kg sucralfate (SC), gastritic mice with 16 g/㎏ Flaxseed oil (FO), gastritic mice with FO + 50 mg/kg Chrysanthemi Indici Flos (FCL), and gastritic mice with FO + 100 mg/kg Chrysanthemi Indici Flos (FCH). Then, mice were orally administered with 150 mM HCl/60% ethanol and caused acute gastritis. After 1 hr, mice were sacrificed, and blood and stomach tissue were collected. Results : Administration of FCL and FCH to mice prior to the induction of gastritis was found to reduce gastric injury. reactive oxygen species (ROS) and peroxy nitrite ($ONOO^-$) levels of stomach tissues were significantly decreased in FO, FCL, and FCH compared to Veh group. As results of stomach protein analyses, FCL and FCH effectively reduce inflammatory-related factors such as inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and interleukin 1 beta ($IL-1{\beta}$) in gastric lesion mice. In addition, nuclear factor kappa B p65 ($NF-{\kappa}B$ p65) and phosphorylation inhibitor of nuclear factor kappa $B{\alpha}(p-I{\kappa}B{\alpha})$ were down-regulated in FCL and FCH administrated gastric lesion mice. Conclusions : These results suggest that FCL and FCH has an inhibitory effect against gastric injury. Therefore, FCL and FCH has the potential to be used as a natural therapeutic drug.

Keywords

DHBCBU_2018_v33n6_19_f0001.tif 이미지

Fig. 1. Scavenging activity of CIL on DPPH and ABTS free radical.

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Fig. 2. Optical change stomach tissues of HCl/ethanol-induced acute gastritic mice.

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Fig. 3. Oxidative stress biomarker in stomach tissues. Oxidative stress biomarker reactive oxygen species (ROS) and produces peroxynitrite (ONOO-) in stomach tissues.

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Fig. 4. Expression of anti-oxidant enzyme of stomach tissues in HCl/ethanol induced acute gastritis mice.

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Fig. 5 Expression of inflammtion-related proteins of stomach tissues in HCl/ethanol induced acute gastritis mice.

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Fig. 6. Expression of inflammtion-related proteins of stomach tissues in HCl/ethanol induced acute gastritis mice.

Table 1. Total Polyphenol and Total Flavonoid Contents of Chrysanthemi Indici Flos

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