Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Anti-inflammatory and Immune Regulatory Effects of Aucklandia lappa Decne 70% Ethanol Extract

운목향 70% 에탄올 추출물의 항염증 및 면역조절에 대한 효과

  • Kim, Min Sun (Department of Food Biotechnology, Woosuk University) ;
  • Kim, Nam Seok (Department of Food Biotechnology, Woosuk University) ;
  • Kwon, Jin (Department of Prosthetics and Orthotics, Korea National University of Welfare) ;
  • Kim, Ha Rim (Department of Food Biotechnology, Woosuk University) ;
  • Lee, Da Young (Department of Food Biotechnology, Woosuk University) ;
  • Oh, Mi Jin (Korea Food Research Institute) ;
  • Kim, Hong Jun (Department of Korean Medicine, Woosuk University) ;
  • Lee, Chang Hyun (Department of Korean Medicine, Woosuk University) ;
  • Oh, Chan Ho (Department of Food Biotechnology, Woosuk University)
  • 김민선 (우석대학교 식품생명공학과) ;
  • 김남석 (우석대학교 식품생명공학과) ;
  • 권진 (한국복지대학교 의료보장구과) ;
  • 김하림 (우석대학교 식품생명공학과) ;
  • 이다영 (우석대학교 식품생명공학과) ;
  • 오미진 (한국식품연구원) ;
  • 김홍준 (우석대학교 한의예과) ;
  • 이창현 (우석대학교 한의예과) ;
  • 오찬호 (우석대학교 식품생명공학과)
  • Received : 2017.10.30
  • Accepted : 2018.01.22
  • Published : 2018.02.28
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Abstract

Background: This present study was conducted to evaluate the anti-inflammatory and immune regulatory effects of Aucklandia lappa Decne (AL). Methods and Results: We measured cytotoxicity, nitric oxide (NO) content, mRNA expression (iNOS, IL-1${\alpha}$, IL-$1{\beta}$, and TNF-${\alpha}$), protein expression (iNOS, COX-2, and $I{\kappa}B$) and phagocytic activity in RAW264.7 cells. Male BALB/c mice were fed 100 mg/kg AL (Aucklandia lappa Decneon 70% ethanol extract) and 250 mg/kg AL for 4 weeks; thereafter, we observed B/T or $CD4^+/CD8^+$ lymphocyte subpopulation change, and expression patterns of $CD4^+$ and $CD8^+$ lymphocytes by immunohistochemical staining in mouse splenocytes and/or thymocytes. To determine the experimental concentration of AL, cell viability was measured by MTT assay and tested at $12.5{\mu}g/m{\ell}$ or less. AL inhibited the levels of NO, lymphokine production (IL-$1{\beta}$, and TNF-${\alpha}$), and mRNA (iNOS, IL-1${\alpha}$, IL-$1{\beta}$, and TNF-${\alpha}$) and protein (iNOS, and COX-2) expression. Additionally, the levels of $I{\kappa}B$, phagocytic activity, and splenic and thymic T lymphocytes, especially $T_H$ and $T_C$ cells were significantly increased in AL administered mice. The immuno-reactive density of $CD4^+$ and $CD8^+$ lymphocytes was stronger in AL groups than in the normal group. AL stimulated NO, iNOS, and COX-2, and regulated IL-1${\alpha}$, IL-$1{\beta}$, TNF-${\alpha}$, and $I{\kappa}B$ in macrophages treated with LPS (lipopolysaccharide). In addition, AL increased the phagocytic activity of macrophages and the immunity of mouse T ($T_H$, and $T_C$) cells. Conclusions: These results suggested that AL might show anti-inflammatory activity via the suppression of various inflammatory markers and immuno-regulatory activity.

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References

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