Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Development of Multiplex Polymerase Chain Reaction Assay for Identification of Angelica Species

Multiplex Polymerase Chain Reaction을 이용한 당귀 종 판별

  • 김용상 (콜마비앤에이치 식품과학연구소) ;
  • 박혁주 (콜마비앤에이치 식품과학연구소) ;
  • 이동희 (경북바이오산업연구원 식품시험검사사업단) ;
  • 김현규 (콜마비앤에이치 식품과학연구소)
  • Received : 2017.10.30
  • Accepted : 2018.01.10
  • Published : 2018.02.28
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Abstract

Background: Angelica gigas, A. sinensis, and A. acutiloba are commercially important in the herbal medicine market, and among them, A. gigas has the highest economic value and price. However, their similar morphological traits are often used for fraud. Despite their importance in herbal medicine, recognition of the differences between Angelica species is currently inadequate. Methods and Results: A multiplex polymerase chain reaction (PCR) method was developed for direct detection and identification of A. gigas, A. sinensis, and A. acutiloba. The gene for the distinction of species was targeted at ITS in the nucleus and trnC-petN gene in chloroplasts. The optimized multiplex PCR in the present study utilized each Angelica species-specific primer pairs. Each primer pair yielded products of 229 base pairs (bp) for A. gigas, 53 bp for A. sinensis, 170 bp for A. acutiloba. Additionally non-specific PCR products were not detected in similar species by species-specific primers. Conclusions: In the present study, a multiplex-PCR assay, successfully assessed the authenticity of Angelica species (A. gigas, A. sinensis, and A. acutiloba). and whole genome amplification (WGA) was performed after DNA extraction to identify, the species in the product. The detection method of raw materials developed in the present study could be applied to herbal medicine and health functional food management.

Keywords

References

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