Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Biological Control using Bacillus toyonensis Strain CAB12243-2 against Soft Rot on Chinese Cabbage

Bacillus toyonensis CAB12243-2 균주를 이용한 배추 무름병의 생물적 방제

  • 김병련 (충청남도농업기술원 친환경농업과) ;
  • 박명수 (서울대학교 생명과학부) ;
  • 한광섭 (충청남도농업기술원 친환경농업과) ;
  • 함수상 (충청남도농업기술원 친환경농업과) ;
  • 박인희 (충청남도농업기술원 친환경농업과) ;
  • 송재경 (국립농업과학원 농업미생물과)
  • Received : 2017.10.26
  • Accepted : 2018.01.15
  • Published : 2018.02.28
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Abstract

Pectobacterium carotovorum subsp. carotovorum was found to be highly virulent to various vegetables, including Chinese cabbage. The antibacterial isolate CAB12243-2 was tested in a field bioassay for suppressing soft rot disease. The nucleotide sequencing of the 16S rRNA gene identified, the CAB12243-2 strain used in this study as Bacillus toyonensis. B. toyonensis CAB12243-2 inhibited the pectate lyase process by soft rot pathogens, and used trehalose and glucose as carbon sources. In field tests, the antibacterial isolate B. toyonensis CAB12243-2 suppressed soft rot disease with 73.0% control efficacy on the spring cultivar "Norangbom" and with 68.9% efficacy on the fall cultivar "Bulam 3". These results suggest that B. toyonensis CAB12243-2 can be used as a biological control agent for the control of soft rot diseases on vegetables.

Pectobacterium carotovorum subsp. carotovorum은 배추를 포함한 대부분의 채소작물에 강한 병원성을 나타낸다. 포장 방제 효과 검정을 통하여 배추 무름병 억제인자로 선발된 CAB12243-2 균주는 16S rRNA gene의 염기서열 상동성 분석으로 Bacillus toyonensis로 확인되었다. B. toyonensis CAB12243-2 균주는 무름병균의 펙틴분해 작용을 억제하였고 탄소원으로 glucose와 trehalose를 이용하였다. 또한 이 균주는 '노랑봄' 품종으로 봄배추 재배 시 73%, '불암3호' 품종으로 가을배추 재배 시 68.9%의 포장 방제 효과를 보였다. 이러한 결과로 B. toyonensis CAB12243-2 균주는 채소류의 무름병에 대한 효과적인 생물적 방제제로 활용이 가능한 것을 확인할 수 있었다.

Keywords

References

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