Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract

  • Kang, Chul Ho (Department of Physical Therapy, Daejeon Woori Hospital) ;
  • Rhie, Sung Ja (Department of Beauty Care Design, Halla University) ;
  • Kim, Young Chul (Department of Public Health, Graduate School, Keimyung University)
  • Received : 2017.08.21
  • Accepted : 2017.10.16
  • Published : 2018.01.15
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Abstract

The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME ($500{\mu}g/mL$) increased 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging, and superoxide dismutase (SOD)-like antioxidant activities by 36.5, 54.7, and 14.8%, respectively, compared with the control. At $1,000{\mu}g/mL$, these activities increased by 63.7, 70.6, and 20.6%, respectively. MGME ($100{\mu}g/mL$) significantly increased the synthesis of type 1 procollagen by 83.7% compared with control treatment. It also significantly decreased Matrix Metalloproteinase-2 (MMP-2) activity and MMP-1 mRNA expression by 36.5% and 69.5%, respectively; however, it significantly increased laminin-5 mRNA expression by 181.2%. These findings suggest that MGME could protect human skin against photo-aging by attenuating oxidative damage, suppressing MMP expression and/or activity as well as by stimulating collagen synthesis.

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References

  1. Brennan, M., Bhatti, H., Nerusu, K.C., Bhagavathula, N., Kang, S., Fisher, G.J., Varani, J. and Voorhees, J.J. (2003) Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem. Photobiol., 78, 43-48. https://doi.org/10.1562/0031-8655(2003)078<0043:MMITMC>2.0.CO;2
  2. Inomata, S., Matsunaga, Y., Amano, S., Takada, K., Kobayashi, K., Tsunenaga, M., Nishiyama, T., Kohno, Y. and Fukuda, M. (2003) Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse. J. Invest. Dermatol., 120, 128-134. https://doi.org/10.1046/j.1523-1747.2003.12021.x
  3. Buechner, N., Schroeder, P., Jakob, S., Kunze, K., Maresch, T., Calles, C., Krutmann, J. and Haendeler, J. (2008) Changes of MMP-1 and collagen type I alpha1 by UVA, UVB and IRA are differentially regulated by Trx-1. Exp. Gerontol., 43, 633-637. https://doi.org/10.1016/j.exger.2008.04.009
  4. Dong, K.K., Damaghi, N., Picart, S.D., Markova, N.G., Obayashi, K., Okano, Y., Masaki, H., Grether-Beck, S., Krutmann, J., Smiles, K.A. and Yarosh, D.B. (2008) UV-induced DNA damage initiates release of MMP-1 in human skin. Exp. Dermatol., 17, 1037-1044. https://doi.org/10.1111/j.1600-0625.2008.00747.x
  5. Amano, S., Ogura, Y., Akutsu, N., Matsunaga, Y., Kadoya, K., Adachi, E. and Nishiyama, T. (2005) Protective effect of matrix metalloproteinase inhibitors against epidermal basement membrane damage: skin equivalents partially mimic photoaging process. Br. J. Dermatol., 153, 37-46.
  6. Aumailley, M. and Rousselle, P. (1999) Laminins of the dermo-epidermal junction. Matrix Biol., 18, 19-28. https://doi.org/10.1016/S0945-053X(98)00004-3
  7. Miyazaki, K., Kikkawa, Y., Nakamura, A., Yasumitsu, H. and Umeda, M. (1993) A large cell-adhesive scatter factor secreted by human gastric carcinoma cells. Proc. Natl. Acad. Sci. U.S.A., 90, 11767-11771. https://doi.org/10.1073/pnas.90.24.11767
  8. Varani, J., Warner, R.L., Gharaee-Kermani, M., Phan, S.H., Kang, S., Chung, J.H., Wang, Z.Q., Datta, S.C., Fisher, G.J. and Voorhees, J.J. (2000) Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin. J. Invest. Dermatol., 114, 480-486. https://doi.org/10.1046/j.1523-1747.2000.00902.x
  9. Brisaert, M. and Plaizier-Vercammen, J. (2000) Investigation on the photostability of a tretinoin lotion and stabilization with additives. Int. J. Pharm., 199, 49-57. https://doi.org/10.1016/S0378-5173(00)00366-5
  10. Kim, B.H., Lee, Y.S. and Kang, K.S. (2003) The mechanism of retinol-induced irritation and its application to anti-irritant development. Toxicol. Lett., 146, 65-73. https://doi.org/10.1016/j.toxlet.2003.09.001
  11. Hwang, J.Y., Lee, C.M. and Kim, Y.C. (2015) Anti-wrinkle efficacy of Stachys riederi var. japonica ethanol extract in human dermal fibroblasts. J. Invest. Cosmetol., 11, 293-301. https://doi.org/10.15810/jic.2015.11.4.002
  12. Kim, S.E., Lee, C.M. and Kim, Y.C. (2016) Anti-wrinkle efficacy of Oenothera laciniata methanol extract in human dermal fibroblasts. J. Invest. Cosmetol., 12, 197-203. https://doi.org/10.15810/jic.2016.12.3.001
  13. Hamburger, M., Adler, S., Baumann, D., Forg, A. and Weinreich, B. (2003) Preparative purification of the major anti-inflammatory triterpenoid esters from Marigold (Calendula officinalis). Fitoterapia, 74, 328-338. https://doi.org/10.1016/S0367-326X(03)00051-0
  14. Bashir, S. and Gilani, A.H. (2008) Studies on the antioxidant and analgesic activities of Aztec marigold (Tagetes erecta) flowers. Phytother. Res., 22, 1692-1694. https://doi.org/10.1002/ptr.2550
  15. Khan, M.T. and Evans, F.J. (1996) Clinical evaluation of Tagetes erecta in the treatment of parakeratosis. Phytother. Res., 10, 186-188.
  16. Gutierrez, R.M.P., Luna, H.H. and Garrido, S.H. (2006) Antioxidant activity of Tagetes erecta essential oil. J. Chil. Chem. Soc., 51, 883-886.
  17. Folin, O. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem., 12, 239-249.
  18. Davis, R., Massey, R.C. and Mcweeny, D.J. (1980) The catalysis of the N-nitrosation of secondary amines by nitrosophenols. J. Food Chem., 6, 115-122. https://doi.org/10.1016/0308-8146(80)90027-8
  19. Perez, G.R.M., Vargas, S.R., Martinez, M.F.J. and Cordova, R.I. (2004) Antioxidant and free radical scavenging activities of 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone from Brickellia veronicaefolia. Phytother. Res.,18, 428-430. https://doi.org/10.1002/ptr.1445
  20. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Marklund, S. and Marklund, G. (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  22. Son, E.D., Choi, G.H., Kim, H., Lee, B., Chang, I.S. and Hwang, J.S. (2007) Alpha-ketoglutarate stimulates procollagen production in cultured human dermal fibroblasts, and decreases UVB-induced wrinkle formation following topical application on the dorsal skin of hairless mice. Biol. Pharm. Bull., 30, 1395-1399. https://doi.org/10.1248/bpb.30.1395
  23. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
  24. Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M. and Merillo, J.M. (2009) Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J. Agric. Food Chem., 57, 1768-1774. https://doi.org/10.1021/jf803011r
  25. Lee, C.J., Chen, L.G., Chang, T.L., Ke, W.M., Lo, Y.F. and Wang, C.C. (2011) The correlation between skin-care effects and phytochemical contents in Lamiaceae plants. Food Chem., 124, 833-841. https://doi.org/10.1016/j.foodchem.2010.07.003
  26. Pokorny, J., Yanishlieva, N. and Gordon, M. (2001) Antioxidants in food: practical applications. Woodhead Publishing Limited, Cambridge, pp. 22-70.
  27. Xu, L.W., Juan, C., Qi, H.Y. and Shi, Y.P. (2012) Phytochemicals and their biological activities of plants in Tagetes L. Chinese Herbal Medicines, 4, 103-117.
  28. Kaisoon, O., Siriamornpun, S., Weerapreeyakul, N. and Meeso, N. (2011) Phenolic compounds and antioxidant activities of edible flowers from Thailand. J. Funct. Foods, 3, 88-99. https://doi.org/10.1016/j.jff.2011.03.002
  29. Li, W., Gao, Y., Zhao, J. and Wang, Q. (2007) Phenolic, flavonoid and lutein ester content and antioxidant activity of 11 cultivars of Chinese marigold. J. Agric. Food Chem., 55, 8478-8484. https://doi.org/10.1021/jf071696j
  30. Mishra, K., Ojha, H. and Chaudhury, N.K. (2012) Estimation of antiradical properties of antioxidants using DPPH assay: a critical review and results. Food Chem., 130, 1036-1043. https://doi.org/10.1016/j.foodchem.2011.07.127
  31. Cano, A., Acosta, M. and Arnaro, M.B. (2000) A method to measure antioxidant activity in organic media: application to lipophilic vitamins. Redox Rep., 5, 365-370.
  32. Gutierrez, R.M.P., Luna, H.H. and Garrido, S.H. (2006) Antioxidant activity of Tagetes erecta essential oil. J. Chil. Chem. Soc., 51, 883-886.
  33. Chen, X., Zhang, E., Fang, L., Zhang, J., Zhu, J., He, W. and Luo, X. (2013) Repair effects of exogenous SOD on Bacillus subtilis against gamma radiation exposure. J. Environ. Radioact., 126, 259-263. https://doi.org/10.1016/j.jenvrad.2013.09.005
  34. Fisher, G.J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S. and Voorhees, J.J. (2002) Mechanisms of photoaging and chronological skin aging. Arch. Dermatol., 138, 1462-1470.
  35. Chaqour, B., Seite, S., Coutant, K., Fourtanier, A., Borel, J.P. and Bellon, G. (1995) Chronic UVB- and all-trans retinoicacid-induced qualitative and quantitative changes in hairless mouse skin. J. Photochem. Photobiol. B, 28, 125-135. https://doi.org/10.1016/1011-1344(94)07080-8
  36. Chung, J.H. (2003) Photoaging in Asians. Photodermatol. Photoimmunol. Photomed., 19, 109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x
  37. Moon, H.J., Lee, S.R., Shim, S.N., Jeong, S.H., Stonik, V.A., Rasskazov, V.A., Zvyagintseva, T. and Lee, Y.H. (2008) Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts. Biol. Pharm. Bull., 31, 284-289.
  38. Maity, N., Nema, N.K., Abedy, M.K., Sarkar, B.K. and Mukherjee, P.K. (2011) Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J. Ethnopharmacol., 137, 1300-1305. https://doi.org/10.1016/j.jep.2011.07.064
  39. Schneider, H., Muhle, C. and Pacho, F. (2007) Biological function of laminin-5 and pathogenic impact of its deficiency. Eur. J. Cell Biol., 86, 701-717. https://doi.org/10.1016/j.ejcb.2006.07.004
  40. Seo, M.Y., Chung, S.Y., Choi, W.K., Seo, Y.K., Jung, S.H., Park, J.M., Seo, M.J., Park, J.K., Kim, J.W. and Park, C.S. (2009) Anti-aging effect of rice wine in cultured human fibroblasts and keratinocytes. J. Biosci. Bioeng., 107, 266-271. https://doi.org/10.1016/j.jbiosc.2008.11.016

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