Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antiaging and antioxidant activity of Hypsizygus marmoreus extracts

흰만가닥버섯(Hypsizygus marmoreus)추출물의 항노화 및 항산화 활성

  • Kwon, Hye-Jin (Department of Chemical Engineering, Soongsil University)
  • Received : 2018.12.09
  • Accepted : 2018.12.17
  • Published : 2018.12.31
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Abstract

This study was carried out to investigate the effects of the extracts of 80% EtOH and EtOAc, BuOH, and D.W in Korean native Hypsizygus marmoreus. Antioxidation and antioxidant analysis to provide the possibility of a multifunctional cosmetic raw material. As a result of analyzing the cytotoxicity of the sample using the animal cell line HaCaT cell, it was confirmed that the toxicity of the sample hardly affected the cells. The inhibition rate of elastase was 30.41% at 10% of sample concentration and inhibition activity of collagenase was 11.65% at 1% of sample concentration. Hypsizygus marmoreus showed better antioxidant activity than similar mushroom. The total phenol contents of the fractions were 778.4 mg and 2.59 mg per 1 g of white mushroom, confirming its potential as a natural antioxidant. As a result, the extract of Hypsizygus marmoreus is considered to be highly valuable as a natural cosmetic material having antioxidant and antioxidant functions.

본 연구는 국내 자생 흰만가닥버섯(Hypsizygus marmoreus)을 시료로 선정하여 80% EtOH 추출 및 EtOAc, BuOH, D.W. 분획믈로 항노화 및 항산화 분석을 통해 복합 기능성 화장품 원료로서의 가능성을 제시하고자 하였다. 동물세포주인 HaCaT cell을 이용하여 시료의 세포 독성을 분석한 결과 시료의 독성은 세포에 거의 영향을 주지 않는 것으로 확인되었다. 시료농도 10%에서 30.41%의 elastase 저해율을 보였고 collagenase 저해활성은 시료농도 1%에서 11.65%의 저해율을 보여 유사 버섯류보다 우수한 항노화 활성을 보였다. 분획물의 총 페놀 함량은 778.4 mg, 흰만가닥버섯 1 g당 2.59 mg으로 천연 항산화제로서의 가능성을 확인 할 수 있었다. 이상의 결과로 흰만가닥버섯 추출물은 항노화 및 항산화 기능의 천연 화장품 소재로 활용 가치가 높을 것으로 판단된다.

Keywords

HGOHBI_2018_v35n4_1081_f0001.png 이미지

Fig. 1. Elastase inhibitory activity & Collagenase inhibitory activity of Samples were incubated at 0.1, 1, 10 and 100%.

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Fig. 2. chromatogram (a)EtOAc fr. (b)BuOH fr.

Table 1. Cell viability assay_ HaCaT cell

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Table 2. Total phenol and flavonoid contents of extracts from Hypsizygus marmoreus

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