Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Comparison of the Effects of Deer Antler, Old Antler, and Antler Glue on Osteoporosis in Ovariectomized Rats

  • Park, Hae In (Department of Acupuncture & Moxibustion medicine, College of Oriental Medicine, Sangji University) ;
  • Lee, Kwang Ho (Department of Acupuncture & Moxibustion medicine, College of Oriental Medicine, Sangji University)
  • Received : 2018.01.15
  • Accepted : 2018.02.09
  • Published : 2018.02.28
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Abstract

Background: Examination of the effects of deer antler, old antler, and antler glue on postmenopausal osteoporosis in an ovariectomized Sprague-Dawley rat model. Methods: The study involved 7 experimental groups; SHAM (sham-operated rats), OVX (ovariectomized rats), E2 (ovariectomized rats with estradiol $10{\mu}g/kg$ daily, orally), DA (ovariectomized rats with deer antler extract 5.83 mg/kg), OA (ovariectomized rats with old antler extract 3.8 mg/kg), low-AG (ovariectomized rats with low dose of antler glue powder 12.5 mg/kg), high-AG (ovariectomized rats with high dose of antler glue powder 37.5 mg/kg). After 6 weeks of treatment, body weight, blood calcium, phosphorus, estradiol, liver [alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT)] and kidney [blood urea nitrogen (BUN)/creatinine ratio] function, and femoral bone mineral density (BMD) were measured. Results: The body weights of DA, OA, low-AG, and high-AG groups did not significantly differ from OVX group. Blood estradiol levels were significantly increased in the DA, low-AG, and high-AG groups compared to the OVX group. Blood calcium, phosphorus, ALP, AST, and ALT levels and BUN/creatinine ratio did not show significant changes in the DA, OA, low-AG, and high-AG groups. BMDs of the femur, and femoral head and neck were significantly increased in the low-AG group. In the OA group, the BMD of the femoral head and neck was significantly increased. Conclusion: Treatment with deer antler, or antler glue for 6 weeks was effective for increasing estradiol and femoral BMD in ovariectomized rats, suggesting that this may be of therapeutic benefit for osteoporosis.

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References

  1. Siris ES, Adler R, Bilezikian J, Bolognese M, Dawson-Hughes B, Favus MJ, et al. The clinical diagnosis of osteoporosis: a position statement from the National Bone Health Alliance Working Group. Osteoporos Int 2014;25:1439-1443. https://doi.org/10.1007/s00198-014-2655-z
  2. NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy. Osteoporosis prevention, diagnosis, and therapy. JAMA 2001;285:85-95.
  3. Chung HY. Osteoporosis Diagnosis and Treatment 2007. J Korean Endocr Soc 2008;23:76-108. [In Korean] https://doi.org/10.3803/jkes.2008.23.2.76
  4. Kim HS. Comparative Analysis of Changes in Bone Mineral Density of Postmenopausal Women Treated with Anti-osteoporotic Agents. Seoul (Korea): CHUNG-ANG University; 2009. [In Korean]
  5. Meng HY, Qu XB, Li N, Yuan S, Lin Z. Effects of pilose antler and antler glue on osteoporosis of ovariectomized rats. Zhong Yao Cai 2009;32:179-182. [In Chinese]
  6. Hwang JS, Seo BI, Park JH, Roh SS, Kim MR, Kim SM, et al. Effect of Cervi Cornu on treatment of Osteoporosis in Ovariectomized Rats. Kor J Herbology 2010;25:1-10. [In Korean]
  7. Kim JH, Kim HS, Jun JY, Jeong DW, Cho CY, Sohn YJ, et al. Effects of Cuscutae Semen Extract on Prevention of Osteoporosis in Ovariectomized Rats. J Orient Obstetrics Gynecol 2012;25:1-11. [In Korean]
  8. Kim JH, Kim JS. Effects of Lycii Fructus and Lycii Folium Extracts on Osteoporosis in Ovariectomized Rats. J Korean Soc Food Sci Nutr 2014;43:24-29. [In Korean] https://doi.org/10.3746/jkfn.2014.43.1.024
  9. Lim DW, Kim YT. Dried root of Rehmannia glutinosa prevents bone loss in ovariectomized rats. Molecules. 2013;18:5804-5813. https://doi.org/10.3390/molecules18055804
  10. Joo SJ, Park JH, Seo BI. Effects of Korean Corni Fructus On treatment of Osteoporosis In Ovariectomized Rats. Kor J Herbology 2007;22:83-95. [In Korean]
  11. Jun JY. Effects of Zizyphi Spinosae Semen on the Ovariectomized Rat Model of Osteoporosis. Seoul (Korea): Kyung Hee University; 2014. Korean. [In Korean]
  12. Han MK, Won JK, Lee CW, Kim CH, Kwon YD, Song YS, et al. Effects of Yukmijiwhang-tang (Liuweidihuang-tang) with Puerariae Radix on Ovariectomized Osteoporotic Rats. J Kor Med Rehabilitation 2007;17:1-21. [In Korean]
  13. Rho JH. Effect of Daeyoungjeonganokyong On Osteoporosis in Ovariectomized Rats. Daegu (Korea): Haany University; 2002. [In Korean]
  14. KIM JH, Jung SK, Shin BC, Kwon YD, Yeom SR, Song YS, et al. Effects of Samul-tang (Siwu-tang) with Puerariae Radix on Ovariectomized Osteoporotic Rat Models. J Kor Med Rehabilitation 2007;17:89-105. [In Korean]
  15. Oh CS, Lee H. A Study on the Effect of Herbal-acupuncture with Drynariae Rhizoma Infusion Solution at Umgok ($KI_{10}$) on Osteoporotic Rats Induced by Ovariectomy. J Korean Acupunct Moxib Soc 2008;25:71-86. [In Korean]
  16. Shin HW, Lee H. Effects of Phamacopuncture with RUBI FRUCTUS Infusion Solution at $KI_{10}$ on Osteoporotic Rats Induced by Ovariectomy. J Korean Acupunct Moxib Soc 2010;27:99-114. [In Korean]
  17. Kang MW, Hong KE, Kim YI. Effects of the Herbal-acupuncture with Epimedii Herba Extract on Osteoporosis in Ovariectomized ddY Mice. J Korean Acupunct Moxib Soc 2009;26:1-14. [In Korean]
  18. Han SW, Lee YH, Kim CH. A Study on Effects of the Cervi Pantotricuhum Cornu Herb-acupuncture on the Osteoporosis Induced by Ovariectomy in Rats. J Pharmacopunct. 2000;3:177-191. https://doi.org/10.3831/KPI.2000.3.1.177
  19. Jung JY, Kim JH, Kim YI. Effects of Herbal-acupuncture with Artemisiae Vulgaris Folium Extract at $KI_{10}$(Eumgok) on Osteoporosis in Ovariectomized Mice. J Korean Acupunct Moxib Soc 2011;28:117-137. [In Korean]
  20. Kwak HB, Oh JM, Kim JH, Kim DJ, Kwon YM, Kim YK. Effect of Water Extract of Deer Antler in Osteoclast Differentiation. Korean J Oriental Physiol Pathol 2008;22:891-895. [In Korean]
  21. Zhang LZ, Xin JL, Zhang XP, Fu Q, Zhang Y, Zhou QL. The antiosteoporotic effect of velvet antler polypeptides from Cervus elaphus Linnaeus in ovariectomized rats. J Ethnopharmacol 2013;150:181-186. https://doi.org/10.1016/j.jep.2013.08.029
  22. Tseng SH, Sung HC, Chen LG, Lai YJ, Wang KT, Sung CH, et al. Effects of velvet antler with blood on bone in ovariectomized rats. Molecules 2012;17:10574-10585. https://doi.org/10.3390/molecules170910574
  23. Bruce M. Koeppen, Bruce A. Stanton. Berne & Levy Physiology. Seoul (Korea): EPUBLIC; 2009. p. 692-702, 783-797.
  24. Simpson AH, Murray IR. Main differences in osteoporotic fracture models which should I use? Injury 2016;47:S15-20.
  25. Sato S, Inoue T. Pathophysiology Based on Human Structure and Function 5. Seoul (Korea): Koonja; 2014. p. 49-59.
  26. Pavel OR, Popescu M, Novac L, Mogoanta L, Pavel LP, Vicas RM, et al. Postmenopausal osteoporosis - clinical, biological and histopathological aspects. Rom J Morphol Embryol 2016;57:121-130.
  27. Korean Medicine University Editorial Committee. Herbology. Seoul (Korea): Yeongrimsa; 2006. p. 591-595. [In Korean]
  28. Fang J, Yang L, Zhang R, Zhu X, Wang P. Are there differences between Sprague-Dawley and Wistar rats in long-term effects of ovariectomy as a model for postmenopausal osteoporosis? Int J Clin Exp Pathol 2015;8:1491-1502.
  29. Wing RR, Matthews KA, Kuller LH, Meilahn EN, Plantinga PL. Weight gain at the time of menopause. Arch Intern Med 1991;151:97-102. https://doi.org/10.1001/archinte.1991.00400010111016
  30. Lim DW, Kim YT. Anti-osteoporotic effects of Angelica sinensis (Oliv.) Diels extract on ovariectomized rats and its oral toxicity in rats. Nutrients 2014;6:4362-4372. https://doi.org/10.3390/nu6104362
  31. NEO Handbook Editorial Committee. NEO Intern Handbook. Seoul(Korea): Koonja; 2012. p. 136-139, 158-159. [In Korean]
  32. Park JE, Ham JS, Kim HK, Lee CH, Kim DW, Seol KH, et al. Effect of Pig Skin Gelatin Hydrolysates on the Bone Mineral Density of Ovariectomized Rats. Korean J Food Sci Ani Resource 2012;32:234-240. https://doi.org/10.5851/kosfa.2012.32.2.234
  33. Futoshi Shintani. Step to Internal Medicine 6. Seoul(Korea): Jeongdam; 2008. p. 186-189.
  34. YB Lee, HJ Lee, KS Kim, JY Lee, SY Nam, SH Cheon, et al. Evaluation of the Preventive Effect of Isoflavone Extract on Bone Loss in Ovariectomized Rats. Biosci Biotechnol Biochem 2004:68: 1040-1045.
  35. Futoshi Shintani. Step to Internal Medicine 5. Seoul(Korea): Jeongdam; 2008. p. 50-53.
  36. Li T, Wu SM, Xu ZY, Ou-Yang S. Rabbiteye blueberry prevents osteoporosis in ovariectomized rats. J Orthop Surg Res 2014;9:1-7. https://doi.org/10.1186/1749-799X-9-1
  37. Kang SJ, Choi BR, Kim SH, Yi HY, Park HR, Kim DC, et al. Dried Pomegranate Potentiates Anti-Osteoporotic and Anti-Obesity Activities of Red Clover Dry Extracts in Ovariectomized Rats. Nutrients 2015;7:2622-2647. https://doi.org/10.3390/nu7042622
  38. MY Lee, HY Kim, Digar Singh, SH Yeo, SY Baek, YK Park, et al. Metabolite Profiling Reveals the Effect of Dietary Rubus coreanus Vinegar on Ovariectomy-Induced Osteoporosis in a Rat Model. Molecules 2016;21:149.

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