Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Hydrogels to Improve the Safety of Yukhoe (Korean Beef Tartare) by Reducing Psychrotrophic Listeria monocytogenes Cell Counts on Raw Beef Surface

  • Oh, Hyemin (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Kim, Sejeong (Risk Analysis Research Center, Sookmyung Women's University) ;
  • Lee, Soomin (Risk Analysis Research Center, Sookmyung Women's University) ;
  • Ha, Jimyeong (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Lee, Jeeyeon (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Choi, Yukyung (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Lee, Yewon (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Kim, Yujin (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Seo, Yeongeun (Department of Food and Nutrition, Sookmyung Women's University) ;
  • Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)
  • Received : 2018.10.02
  • Accepted : 2018.10.23
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study developed an antimicrobial hydrogel to control Listeria monocytogenes in Yukhoe (Korean beef tartare). Four hydrogels (hydrogel 1: 5% alginate+1% chitosan+0.2% $CaCl_2$, hydrogel 2: 1% ${\kappa}$-carrageenan+1% chitosan, hydrogel 3: 2% ${\kappa}$-carrageenan+1% $CaCl_2$, and hydrogel 4: 2% ${\kappa}$-carrageenan+3% $CaCl_2$) were prepared. The hydrogels then absorbed 0.1% grapefruit seed extract (GSE) and 0.1% citrus extract (CE) for 30, 60, 120, and 240 min to be antimicrobial hydrogels. To select the most effective antimicrobial hydrogel, their swelling ratio (SR) and antilisterial activities were determined. The selected hydrogel ($2{\times}2cm$) was then placed on surface of beef (round; $3{\times}3cm$), where L. monocytogenes (ca. $10^6CFU/g$) were inoculated, and the cell counts were enumerated on PALCAM agar. Among the hydrogels, the SR of hydrogel 1 increased with absorbing time, but other hydrogels showed no significant changes. Antimicrobial hydrogel 1 showed higher (p<0.05) antilisterial activity than other antimicrobial hydrogels, especially for the one absorbed the antimicrobial for 120 min. Thus, the antimicrobial hydrogel 1 absorbed antimicrobials for 120 min was applied on raw beef at $4^{\circ}C$, and reduced (p<0.05) more than 90% of L. monocytogenes on raw beef. These results indicate that antimicrobial hydrogel 1 formulated with 0.1% GSE or 0.1% CE is appropriate to improve the safety of Yukhoe by reducing psychrotrophic L. monocytogenes cell counts on raw beef.

Keywords

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : Rural Development Administration

References

  1. Amsden B, Turner N. 1999. Diffusion characteristics of calcium alginate gels. Biotechnol Bioeng 65:605-610. https://doi.org/10.1002/(SICI)1097-0290(19991205)65:5<605::AID-BIT14>3.0.CO;2-C
  2. Andritsos ND, Mataragas M, Paramithiotis S, Drosinos EH. 2013. Quantifying Listeria monocytogenes prevalence and concentration in minced pork meat and estimating performance of three culture media from presence/absence microbiological testing using a deterministic and stochastic approach. Food Microbiol 36:395-405. https://doi.org/10.1016/j.fm.2013.06.020
  3. Bajpai AK, Shukla SK, Bhanu S, Kankane S. 2008. Responsive polymers in controlled drug delivery. Prog Polym Sci 33:1088-1118. https://doi.org/10.1016/j.progpolymsci.2008.07.005
  4. Campo VL, Kawano DF, da Silva DB, Ivone C. 2009. Carrageenans: Biological properties, chemical modifications and structural analysis-A review. Carbohydr Polym 77:167-180. https://doi.org/10.1016/j.carbpol.2009.01.020
  5. Carpentier B, Cerf O. 2011. Review-persistence of Listeria monocytogenes in food industry equipment and premises. Int J Food Microbiol 145:1-8. https://doi.org/10.1016/j.ijfoodmicro.2011.01.005
  6. Centers for Disease Control and Prevention [CDC]. 2013. Vital signs: Listeria illnesses, deaths, and outbreaks-United States, 2009-2011. Morb Mortal Wkly Rep 62:448-452.
  7. Chiarini E, Tyler K, Farber JM, Pagotto F, Destro MT. 2009. Listeria monocytogenes in two different poultry facilities: Manual and automatic evisceration. Poult Sci 88:791-797. https://doi.org/10.3382/ps.2008-00396
  8. Cvetnic Z, Vlanimir-knezevic S. 2004. Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharm 54:243-250.
  9. Ganji F, Vasheghani-Farahani S, Vasheghani-Farahani E. 2010. Theoretical description of hydrogel swelling: A review. Iran Polym J 19:375-398.
  10. Indrawattana N, Nibaddhasobon T, Sookrung N, Chongsa-nguan M, Tungtrongchitr A, Makino S, Tungyong W, Chaicumpa W. 2011. Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods. J Health Popul Nutr 29:26-38.
  11. Kamoun EA, Kenawy ES, Chen X. 2017. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J Adv Res 8:217-233. https://doi.org/10.1016/j.jare.2017.01.005
  12. Khen BK, Lynch OA, Carroll J, McDowell DA, Duffy G. 2015. Occurrence, antibiotic resistance and molecular characterization of Listeria monocytogenes in the beef chain in the Republic of Ireland. Zoonoses Public Health 62:11-17.
  13. Kim H, Oh H, Lee H, Kim S, Ha J, Lee J, Choi Y, Lee Y, Kim Y, Yoon Y, Lee S. 2018. Antimicrobial effect of phytochemicals to Listeria monocytogenes isolated from slaughterhouses. J Food Hyg Saf 33:255-258. https://doi.org/10.13103/JFHS.2018.33.4.255
  14. Kim S, Oh H, Lee H, Lee S, Ha J, Lee J, Choi Y, Yoon Y. 2017. Polymer hydrogels formulated with various cross-linkers for food-surface application to control Listeria monocytogenes. J Food Hyg Saf 32:443-446. https://doi.org/10.13103/JFHS.2017.32.5.443
  15. Kojima M, Ando S, Kataoka K, Hirota T, Aoyagi K, Nakagami H. 1998. Magnetic resonance imaging (MRI) study of swelling and water mobility in micronized low-substituted hydroxypropylcellulose matrix tablets. Chem Pharm Bull 46:324-328. https://doi.org/10.1248/cpb.46.324
  16. Lakicevic B, Nastasijevic I, Raseta M. 2015. Sources of Listeria monocytogenes contamination in retail establishments. Procedia Food Sci 5:160-163. https://doi.org/10.1016/j.profoo.2015.09.046
  17. Lee TW, Kim JC, Hwang SJ. 2003. Hydrogel patches containing triclosan for acne treatment. Eur J Pharm Biopharm 56:407-412. https://doi.org/10.1016/S0939-6411(03)00137-1
  18. Mohamadnia Z, Zohuriaan-Mehr MJ, Kabiri K, Jamshidi A, Mobedi H. 2008. Ionically cross-linked carrageenan-alginate hydrogel beads. J Biomater Sci Polym Ed 19:47-59. https://doi.org/10.1163/156856208783227640
  19. Oh H. 2017. Prevalence and genetic characteristics of Listeria monocytogenes isolates from slaughterhouses, farms, and humans in South Korea. Master's Thesis, Sookmyung Women's Univ., Seoul, Korea.
  20. Pascalau V, Popescu V, Popescu GL, Dudescu MC, Borodi G, Dinescu AM, Moldovan M. 2013. Obtaining and characterizing alginate/k-carrageenan hydrogel cross-linked with adipic dihydrazide. Adv Mater Sci Eng 2013:380716.
  21. Pesavento G, Ducci B, Nieri D, Comodo N, Nostro AL. 2010. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 21:708-713. https://doi.org/10.1016/j.foodcont.2009.10.012
  22. Rhoades JR, Duffy G, Koutsoumanis K. 2009. Prevalence and concentration of verocytotoxigenic Escherichia coli, Salmonella enterica and Listeria monocytogenes in the beef production chain: A review. Food Microbiol 26:357-376. https://doi.org/10.1016/j.fm.2008.10.012
  23. Samelis J, Metaxopoulos J. 1999. Incidence and principal sourced of Listeria spp. and Listeria monocytogenes contamination in processed meats and a meat processing plant. Food Microbiol 16:465-477. https://doi.org/10.1006/fmic.1998.0263
  24. Schafer DF, Steffens J, Barbosa J, Zeni J, Paroul N, Valduga E, Junges A, Backes GT, Cansian RL. 2017. Monitoring of contamination sources of Listeria monocytogenes in a poultry slaughterhouse. LWT-Food Sci Technol 86:393-398. https://doi.org/10.1016/j.lwt.2017.08.024
  25. Theivendran S, Hettiarachchy NS, Johnson MG. 2006. Inhibition of Listeria monocytogenes by nisin combined with grape seed extract or green tea extract in soy protein film coated on Turkey frankfurters. J Food Sci 71:M39-M44. https://doi.org/10.1111/j.1365-2621.2006.tb08905.x
  26. Trad M, Miled W, Benlroufa S, Boughattas A, Benslama R, Fayala F, Bakhrouf A. 2018. Chitosan hydrogel-coated cotton fabric: Antibacterial, pH-responsiveness, and physical properties. J Appl Polym Sci 135:46645. https://doi.org/10.1002/app.46645
  27. Vitas AI, Garcia-Jalon VA. 2004. Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). Int J Food Microbiol 90:349-356. https://doi.org/10.1016/S0168-1605(03)00314-3
  28. Wieczorek K, Dmowska K, Osek J. 2012. Prevalence, characterization, and antimicrobial resistance of Listeria monocytogenes isolates from bovine hides and carcasses. Appl Environ Microbiol 78:2043-2045. https://doi.org/10.1128/AEM.07156-11

Cited by

  1. Dynamic model to describe kinetic behavior of Listeria monocytogenes in smoked salmon vol.41, pp.5, 2018, https://doi.org/10.1111/jfs.12925
  2. Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder vol.11, pp.1, 2018, https://doi.org/10.1038/s41598-021-90461-7