Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Chemical identification and antioxidant activity of phenolic compounds extracted from the fruiting body of 'Hankyong Sanghwang', Phellinus linteus KACC 93057P

Phellinus linteus KACC 93057P, '한경상황버섯'인공재배 자실체 페놀추출물의 항산화 활성 및 화학적 동정

  • Min, Gyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Jeong, Ee-un (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Yun, Bong-Sik (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • 민경진 (한경대학교 미래융합기술대학원) ;
  • 정이은 (한경대학교 미래융합기술대학원) ;
  • 윤봉식 (전북대학교 생명공학부) ;
  • 강희완 (한경대학교 미래융합기술대학원)
  • Received : 2018.11.21
  • Accepted : 2018.12.11
  • Published : 2018.12.31
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Abstract

The aim of this study was to identify chemical composition and antioxidant activity of phenolic extract of fruiting bodies of an artificially cultivated Hankyong Sanghwang mushroom, Phellinus linteus KACC 93057P (PLHS). The total phenolic content of 60% ethanolic extract of fruiting bodies of two-year-old PLHS grown on Oak wood logs was $19.05{\pm}0.32mg$ gallic acid equivalent (GAE)/g, which was 4-10 times high compared to the other species of mushrooms. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) value of PLHS were 2-10 and 5 times higher, respectively, than those of the other species of mushrooms. High performance liquid chromatography (HPLC) analysis of ethyl acetate fraction of 70% methanolic extract of the fruiting bodies of PLHS revealed the presence of styrylpyrone-class compounds, davallialactone, hispidin, hypholomine B, and caffeic acid, a compound of the phenylpropanoid pathway.

참나무 단목원목으로 재배한 P. linteus KACC93057P(PLHS)2년산 자실체 60%에탄올 추출물의 총 페놀함량은 $19.05{\pm}0.32mg\;GAE/g$로 다른 비교구 버섯 종에 비하여 4배에서 10배이상의 높게 나타났다. $125{\mu}g/mL$농도에서 DPPH와 ABTS 라디칼 소거능은 62%와 100%를 나타냈으며 FRAP 환원력은 $0.7{\mu}MFeSO_4/g$으로 비교 구 버섯종 보다 2에서 10배이상의 유효활성이 검출 되었다. PLHS 자실체 ethyl acetate분배 추출물의 HPLC분석에서 styrylpyrone 계열의 화합물 davallialactone, hispidin, hypholomine B와 phenylpropanoid 계열의 화합물인 caffeic acid가 동정 되었다.

Keywords

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Fig. 1. Morphological characteristics of fruiting bodies of Phellinus linteus KACC93057P(PLHS) and Phellinus baumii (PBJS) formed on oak logs after two years of cultivation.

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Fig. 2. HPLC chromatogram of ethyl acetate layer extracted from fruiting body of Phellinus linteus KACC93057P. 1. Davallialactone, 2. Hispidin, 3. Hypholomine B, 4. Caffeic acid

Table 1. Total polyphenol content of different mushroom species and Phellinus linteus KACC93057P

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Table 2. DPPH radical scavenging activity of different mushroom species and Phellinus linteus KACC93057P

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Table 3. ABTS radical scavenging activity of different mushroom species and Phellinus linteus KACC93057P

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Table 4. FRAP value of different mushroom species and Phellinus linteus KACC93057P

BSHGBD_2018_v16n4_311_t0004.png 이미지

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