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The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Comparison of Volatile Fatty Acids, Monosaccharide Analysis and Metabolic Profiling in Rumen Fluid According to Feeding Methods

사료 급여 방식에 따른 반추위액 내 휘발성지방산과 단당류 비교 분석과 대사산물 분석

  • Eom, Jun-Sik (Division of Applied Life Science(BK21Plus), Gyeongsang National University) ;
  • Lee, Shin-Ja (Institue of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University) ;
  • Lee, Yoo-Gyung (Animal Nutrition and Physiology Team, National Institute of Animal Science) ;
  • Lee, Sung-Sill (Division of Applied Life Science(BK21Plus), Gyeongsang National University)
  • 엄준식 (경상대학교 응용생명과학부(BK21Plus)) ;
  • 이신자 (경상대학교 농업생명과학연구원(중점연구소)) ;
  • 이유경 (국립축산과학원 영양생리팀) ;
  • 이성실 (경상대학교 응용생명과학부(BK21Plus))
  • Received : 2018.10.29
  • Accepted : 2018.12.07
  • Published : 2018.12.31
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Abstract

This study was conducted to investigate VFA, monosaccharides and metabolites in rumen fluid according to feeding methods. Three castrated Hanwoo steers were used to the $3{\times}3$ Latin square design, 10 day for the diet adaptation period. VFA and monosaccharides which were not detected by HPLC and HPAEC however, those were detected by $^1H-NMR$. Among the metabolites measured by $^1H-NMR$ carbohydrate metabolites, pyruvate was detected only in the rumen fluid before feeding and succinate was detected before and after feeding rumen fluid. In amino acid total 9 metabolites were detected. In lipid metabolites, ethylene glycol was significantly higher (P<0.05) in before feeding Con group. In aliphatic acylic metabolite, trimethylamine N-oxide was no significant difference observed compare to Con group. In this study, many metabolites were observed in the rumen fluid by $^1H-NMR$, and it confirmed that rumen metabolic products were changed by feeding methods.

거세한우 3두를 $3{\times}3$ Latin square design 시험방법에 적용하여 사료 적응 기간 10일 후 사료 급여 방식에 따른 반추위액 내 휘발성지방산과 단당류 비교 분석 및 대사산물 분석에 대한 연구를 수행하였다. 연구 결과 HPLC와 HPAEC에서 측정되지 않은 휘발성지방산과 단당류들은 $^1H-NMR$에서는 측정이 가능 한 것을 알 수 있었다. $^1H-NMR$에서 측정된 대사산물 중, carbohydrate 계열 대사산물 pyruvate는 사료 급여 전 반추위액에서만 측정 되었으며, succinate는 사료 급여 전 후에서 모두 측정 되었다. Amino acid 계열 대사산물은 총 9가지가 측정 되었다. Lipid 계열 대사산물 ethylene glycol는 사료 급여 전 Con구에서 유의적(P<0.05)으로 높게 측정 되었다. Aiphatic acylic compounds 계열 대사산물 trimethylamine N-oxide는 유의적인 차이는 보이지 않았다. 이번 연구를 통해 $^1H-NMR$를 이용하여 반추위액 내 많은 대사산물 측정이 가능한 것을 알 수 있었으며 사료 급여 전 후 급여 방식에 따라 대사산물의 변화가 나타나는 것을 확인 할 수 있었다.

Keywords

SHGSCZ_2018_v19n12_814_f0001.png 이미지

Fig. 1. The Representative Spectrum 1H-NMR Obtained from Rumen Fluid Samples. The Spectra were Constructed Using TSP at 0.00 ppm as Reference

SHGSCZ_2018_v19n12_814_f0002.png 이미지

Fig. 2. Continued

Table 1. The Chemical Composition of Rice Straw, Timothy and Concentrate Used in the Experiment

SHGSCZ_2018_v19n12_814_t0001.png 이미지

Table 2. Concentration of Volatile Fatty Acids in Rumen Fluid by High Performance Liquid Chromatography and 1H-Nuclear Magnetic Resonance spectroscopy

SHGSCZ_2018_v19n12_814_t0002.png 이미지

Table 3. Concentration of Monosaccharide in Rumen Fluid by High Performance Anion-Exchange Chromatography and 1H-Nuclear Magnetic Resonance spectroscopy

SHGSCZ_2018_v19n12_814_t0003.png 이미지

Table 4. List of Metabolites in Rumen Fluid According to Different Feeding Methods and Fermentation Time

SHGSCZ_2018_v19n12_814_t0004.png 이미지

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