The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Modulatory Effects of Herbal Medicines Extracts on Cytokine Release in Immune Response of RAW 264.7 and TK-1

한약재 9종의 추출물이 RAW 264.7과 TK-1 세포의 cytokine 분비에 미치는 영향

  • Bae, Su-kyoung (Dept. of Vaccine Biotechnology, Andong National University) ;
  • Cho, Se-hee (Dept. of Biological Science, Andong National University) ;
  • Ahn, Tae-kyu (Dept. of Vaccine Biotechnology, Andong National University) ;
  • Kim, Jee-in (Institute of Vaccine Biotechnology, Andong National University) ;
  • Kim, Bong-hyun (Bubu Oriental Medicine Clinic) ;
  • Lim, Jae-hwan (Dept. of Vaccine Biotechnology, Andong National University)
  • Received : 2018.12.10
  • Accepted : 2019.01.01
  • Published : 2018.12.30
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Abstract

Objectives: The purpose of this study is to determine the stimulatory effects of herbal medicines extracts on cytokines release of immune response in immune cells, RAW 264.7 and TK-1 cell. Methods: In a total of 18 extracts, 9 water extracts and 9 ethanol extracts, of herbal medicines, the quantities of polyphenolic compounds were measured and anti-oxidation activities were determined by colorimetric assay. The herbal medicine extracts were treated on RAW 264.7 and TK-1, respectively, and then the releasing changes of tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$), interleukin-6, and interleukin-10 from both immune cells were determined by the enzyme-linked immunosorbent assay. Results: The polyphenol contents were measured to be 1.56~0.64 mg/g of solids in the two types of extracts with 9 kinds of herbal medicines, while antioxidant activities were found to be 95.62~31.46% as compared with ascorbic acid control. In RAW 264.7 cells treated with herbal medicines extracts, the secretion of $TNF-{\alpha}$ increased to 1.31~1.18 fold, and the amounts of IL-6 were 68.4~97.9% compared with the control group treated with LPS alone. In particular, the secretion amount of anti-inflammatory cytokine IL-10 was suppressed by treatment using herbal medicine extracts. In the case of TK-1 cells, $TNF-{\alpha}$ secretion was suppressed according to the concentrations of herbal extract. The released amounts of IL-10 were shown at 10~40 pg/ml, and increased in a dose-dependent manner. Conclusions: Herbal medicines extracts act on macrophages inducing the secretion of inflammatory cytokine, thereby enhancing the activity of innate immunity. When acting on T cells involved in adaptive immunity, the secretion of anti-inflammatory cytokine is increased to induce the inhibition of the innate immune response.

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References

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