Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Crystal Structure and Biochemical Characterization of Xylose Isomerase from Piromyces sp. E2

  • Son, Hyeoncheol Francis (School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University) ;
  • Lee, Sun-Mi (Clean Energy Research Center, Korea Institute of Science and Technology (KIST)) ;
  • Kim, Kyung-Jin (School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University)
  • Received : 2017.11.15
  • Accepted : 2018.01.18
  • Published : 2018.04.28
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Abstract

Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a ${\text\tiny{D}}$-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize ${\text\tiny{D}}$-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino-acid sequence comparison with other reported XIs and relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XIs. Moreover kinetic analysis of PsXI was also performed using $Mn^{2+}$, the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance ${\text\tiny{D}}$-xylose utilization for biofuel production.

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