Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Inhibition Effect of Enzymatic Hydrolysate from Japanese Mud Shrimp Upogebia major on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells (HUVECs)

혈관내피세포에서 TNF-α로 유도되는 혈관염증에 대한 쏙(Upogebia major) 효소가수분해물의 억제 효과

  • Kim, So-Yeon (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Yang, Ji-Eun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Song, Jae-Hee (Tidal Flat Research Center, National Institute of Fisheries Science) ;
  • Maeng, Sang-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Lee, Ji-Hyun (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Yoon, Na-Young (Food Safety and Processing Research Division, National Institute of Fisheries Science)
  • 김소연 (국립수산과학원 식품위생가공과) ;
  • 양지은 (국립수산과학원 식품위생가공과) ;
  • 송재희 (서해수산연구소 갯벌연구센터) ;
  • 맹상현 (국립수산과학원 식품위생가공과) ;
  • 이지현 (국립수산과학원 식품위생가공과) ;
  • 윤나영 (국립수산과학원 식품위생가공과)
  • Received : 2018.02.06
  • Accepted : 2018.03.02
  • Published : 2018.04.30
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Abstract

Arteriosclerosis is the major cause of coronary artery and cerebrovascular disease, which are leading causes of death. Pro-inflammatory cytokines induce injury to vascular endothelial cells by increasing cell adhesion molecules, leading to vascular inflammation, a major risk factor for the development of arteriosclerosis. In the current study, we investigated the inhibitory effect of enzymatic hydrolysate from Japanese mud shrimp Upogebia major on the inflammation of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$)-stimulated human umbilical vein endothelial cells (HUVECs). We first evaluated the antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of eight U. major enzymatic hydrolysates: alcalase, papain, ${\alpha}$-chymotrypsin (${\alpha}-Chy$), trypsin, pepsin, neutrase, protamex and flavourzyme. Of these, ${\alpha}-Chy$ exhibited potent antioxidant and ACE inhibitory activities. The ${\alpha}-Chy$ hydrolysate was fractionated by two ultrafiltration membranes of 3 and 10 kDa. The ${\alpha}-Chy$ hydrolysate of U. major and its molecular weight cut-off fractions resulted in a significant reduction in NO production and a decrease in cell adhesion molecules [vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and endothelial-selectin (E-selectin)] and pro-inflammatory cytokines [interleukin-6 (IL-6), interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1)] in $TNF-{\alpha}$-stimulated HUVECs. These results suggest that enzymatic hydrolysate from U. major can be used in the control and prevention of vascular inflammation and arteriosclerosis.

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References

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