Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidants and Acetyl-cholinesterase Inhibitory Activity of Solvent Fractions Extracts from Dendropanax morbiferus

황칠나무의 용매 분획별 추출물의 항산화 활성 및 Acetyl-cholinesterase 저해 활성비교

  • Yu, Ji Min (Department of Medicinal Biotechnology, Dong-A University) ;
  • Moon, Hyung In (Department of Medicinal Biotechnology, Dong-A University)
  • 유지민 (동아대학교 의약생명공학과) ;
  • 문형인 (동아대학교 의약생명공학과)
  • Received : 2017.09.29
  • Accepted : 2018.01.25
  • Published : 2018.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The leaves and stems of Dendropanax morbiferus were separated from organic solvents with methanol. The organic solvent fractions were fractionated with dichloromethane, ethyl acetate and butanol according to the systematic fractionation method. Oxidation in the body induces aging, and antioxidant activity has attracted the attention of many people as a preventive component to suppress negative reactions in the body. To investigate the antioxidant activity of Dendropanax morbiferus were subjected to DPPH free radical assay. In addition, acetyl cholinesterase inhibitions were performed for Alzheimer's disease as an aging neurological disease. As a result, it was confirmed that the antioxidant effect of DPPH was generally good in the antioxidant test. The ethyl acetate fractions of Dendropanax morbiferus stems and leaves were $IC_{50}=30{\mu}g/m{\ell}$. Acetyl cholinesterase inhibition experiments were carried out at a concentration of $250{\mu}g/m{\ell}$. Dendropanax morbiferus stems fractions showed dichloromethane fraction of 57.68%, which significantly inhibited the activity of acetyl cholinesterase.

황칠나무 잎과 줄기를 분리하여 각각 methanol 추출하여, 계통분획상법에 따라 dichloromethane, ethyl acetate, butanol로 분획하였다. 분획물로 실시한 TLC에서 알칼로이드 성분으로 분리되는 성분을 확인할 수 있었다. 잎과 줄기 모두 ethyl acetate분획물은 $IC_{50}$ $30{\mu}g/m{\ell}$으로 높게 측정 되었으며, ethyl acetate, dichloromethane, butanol분획물 순으로 저해 활성이 높게 나타났다. Acetyl cholinesterase inhibition assay를 실시한 결과 황칠나무 잎과 줄기 모두 dichloromethane, ethyl acetate, butanol 분획물 순으로 저해 활성이 높은 것으로 나타났으며, 가장 높은 활성을 보인 황칠나무 줄기와 잎의 dichloromethane 분획물의 알칼로이드 함량이 상대적으로 높은 것으로 보아 알칼로이드 성분에 의한 것으로 유추 할 수 있으므로, 알칼로이드 성분의 동정 및 성분구조의 규명을 통하여 AChE저해 활성을 이용한 신경관련 질환에 도움을 줄 수 있는 천연물의 개발이 가능할 것으로 사료된다.

Keywords

References

  1. Abbott, N.J., L. Ronnback and E. Hansson. 2006. Astrocyte-endothelial interactions at the blood-brain barrier. J. Nat Rev Neurosci. 7:41. https://doi.org/10.1038/nrn1824
  2. Albertazzi, P., S.A. Steel, E. Clifford and M. Bottazzi. 2002. Attitudes towards and use of dietary supplementation in a sample of postmenopausal women. J. Climacteric. 5:374-382. https://doi.org/10.1080/cmt.5.4.374.382
  3. Branen, A.L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. American Oil Chem. Soc. 52:59. https://doi.org/10.1007/BF02901825
  4. Choe, S.Y and K.H. Yang. 1982. Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J. Food Sci. Technol. 14: 283-288.
  5. Choi, J.H., K.W. Kim, S.E. Park, H.J. Lee, K.M. Kim, K.J. Kim, M.K. Kim, S.J. Kim and S. Kim. 2015. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera leveille. J. Biosci Bioeng. 120:181-186. https://doi.org/10.1016/j.jbiosc.2014.12.012
  6. Ellman, G.L., K.D. Courtney, V. Andres and R.M. Featherstone. 1961. A new and rapid colorimetric determination of acety- lcholinesterase activity. J. Biochem. Pharmacol. 7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  7. Fang, Y.Z., S. Yang and G. Wu. 2002. Free radical antioxidant and nutrition. Nutr. 18:872-879. https://doi.org/10.1016/S0899-9007(02)00916-4
  8. Gadow, A., E. Joubert and C.F. Hansmann. 1997. Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), r-Tocopherol, BHT, and BHA. J. Agric. Food Chem. 45:632-638. https://doi.org/10.1021/jf960281n
  9. Geldmacher, D.S. 2007. Treatment guidelines for alzheimer's disease: redefining perceptions in primery care. primary care companion. J. Clin Psychiatry 9:113.
  10. Kim, H.J and S.H. Kang. 2017. Ethnobotany, phytochemistry, pharmacology of the Korean campanulaceae: a comprehensive review. Korean J. Plant Res. 30:240-264. https://doi.org/10.7732/kjpr.2017.30.2.240
  11. Kim, J.M., S.K. Park, T.J. Guo, J.Y. Kang, J.S Ha, D.S. Lee, O. Kwon, U. Lee and H.J. Heo. 2016. Neuronal cell protective effect of Dendropanax morbifera extract against high glucose- induced oxidative stress. J. Korean Soc. Food Sci. Nutr. 45:938-947 (in Korean). https://doi.org/10.3746/jkfn.2016.45.7.938
  12. Lee, D.J. and J.Y. Lee. 2004. Antioxidant activity by DPPH assay. Korean J. Crop Sci. 49:187-194.
  13. Musial, A., M. Bajda and B. Malawska. 2007. Recent develop- ments in cholinesterases inhibitors for alzheimer's disease treatmenr. Curr. Med. Chem. 14:2654-2679. https://doi.org/10.2174/092986707782023217
  14. Mo, J.H. and S.J. Oh. 2013. Tyrosinase inhibitory activity and melanin production inhibitory activity of the methanol extract and fractions from Dendropanax morbifera lev. Asian J. Beauty and Cosmetol. 11:275-280 (in Korean).
  15. Nakamura, M., J.H. Ra and J.S Kim. 2016. The comparative analysis of antioxidant and biological activity for the Dendropanax morbifera lev. leaves extracted by different ethanol concentrations. J. Pharmaceutical Soc. Japan. 136: 1285-1296. https://doi.org/10.1248/yakushi.16-00018
  16. Spencer, B., E. Rockenstein, L. Crews, R. Marr and E. Masliah. 2007. Novel strategies for alzheimer's disease treatment. Expert opin. Biol. Ther. 7:1853-1867. https://doi.org/10.1517/14712598.7.12.1853
  17. Seow, D. and S. Gauthier. 2007. Pharmacotherapy of alzheimer disease. Can. J. Psychiatry. 52:620-629. https://doi.org/10.1177/070674370705201003
  18. Son, H.J. and G.H. Ryu. 2009. Chemical compositions and antioxidant activity of extract from a extruded white ginseng. J. Korean Soc. Food Sci. Nutr. 38:946-950 (in Korean). https://doi.org/10.3746/jkfn.2009.38.7.946
  19. Wagner, H. and S. Bladt. 1996. Plant Drug Analysis. A Thin Layer Chromatography Atlas- Alkaloid Drugs. Science & Business Media, Munchen, Germany. pp. 3-51.