Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Detection of QTLs in an Interspecific Cross between Perilla citriodora × P. hirtella Mapping Population

들깨 종간 교잡(Perilla citriodora × P. hirtella) 집단의 양적 형질 유전자좌 분석

  • 이명희 (농촌진흥청 국립식량과학원) ;
  • 오기원 (농촌진흥청 국립식량과학원) ;
  • 김명식 (팜한농(주)) ;
  • 김성업 (농촌진흥청 국립식량과학원) ;
  • 김정인 (농촌진흥청 국립식량과학원) ;
  • 오은영 (농촌진흥청 국립식량과학원) ;
  • 배석복 (농촌진흥청 국립식량과학원) ;
  • 여운상 (농촌진흥청 국립식량과학원) ;
  • 김태호 (농촌진흥청 국립농업과학원) ;
  • 이정희 (씨더스(주)) ;
  • 정찬식 (농촌진흥청 국립식량과학원) ;
  • 곽도연 (농촌진흥청 국립식량과학원) ;
  • 김용철 (농촌진흥청 국립식량과학원)
  • Received : 2017.09.19
  • Accepted : 2017.11.24
  • Published : 2018.03.01
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Abstract

An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of $F_2$ population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of $F_2$ plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.

레몬들깨(P. citriodora)와 범꼬리소엽(P. hirtella) 등 2종의 2배체 야생 들깨 종간 교잡을 통해 들깨 유전체 사업의 연관 유전지도 작성에 필요한 집단을 육성하였다. 두 종의 염색체수는 n=10으로 같고 종간 교잡 후대는 세대를 거치는 동안 정상적인 생육과 결실을 보였다. $F_2$ 집단의 표현형은 정규분포를 보여 종간 교잡 식물체의 감수분열 과정에서 두 종의 염색체가 친화성이 높은 것으로 보였다. $F_2$ 집단으로부터 수확한 종자에서 Rosmarinic acid, Luteolin, Apigenin등 3종의 성분을 분석한 결과 4배체 재배종 들깨의 함량과 유사한 범위에 분포하였고 Apigenin과 Luteolin 간에 0.762의 높은 정의 상관이 있었다. 주요 농업형질 및 기능성분 분석 결과 21개의 QTL을 찾았다. Apigenin과 Luteolin 간에 높은 정상관이 있었으나 QTL 영역은 다르게 조사되었다. 엽색(LC)의 QTL은 3번 염색체에 하나만 존재하였는데 LOD가 14.3으으로 매우 높고 표현형 변이에 대한 QTL의 설명 비율이 50.4%로 높은 부분 우성 형질이었다. 또한 이 QTL 주변 영역에는 안토시아닌 생합성 조절인자로 알려진 basic helix-loop-helix(bHLH) DNA-binding superfamily protein, myb domain protein 43, Transducin/WD40 repeat-like superfamily protein이 확인되었다.

Keywords

Acknowledgement

Grant : 들깨 유전체 해독소재 개발 및 매핑 집단 작성

Supported by : 농촌진흥청

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