Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Variation of Isoflavone Contents and Classification Using Multivariate Analysis in Korean Soybean Varieties Released from 1913 to 2013

1913년부터 2013년까지 한국에서 육성된 콩 품종의 Isoflavone 함량의 변이와 다변량 분석에 의한 품종군 분류

  • Kim, Hyun Myung (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Jang, Eun Kyu (Gyeonggi-do Agricultural Research and Extension Services) ;
  • Gwak, Byeong Sam (Gyeongsangbuk-do Silkworm & Insect Management Center) ;
  • Hwang, Tae Young (National Institute of Animal Science) ;
  • Yun, Geon Sig (Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Hwang, Se Gu (Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Jeong, Heon Sang (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kim, Hong Sig (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 김현명 (충북대학교 농업생명환경대학) ;
  • 장은규 (경기도농업기술원) ;
  • 곽병삼 (경상북도 잠사곤충사업장) ;
  • 황태영 (국립축산과학원 축산자원개발부) ;
  • 윤건식 (충청북도농업기술원) ;
  • 황세구 (충청북도농업기술원) ;
  • 정헌상 (충북대학교 농업생명환경대학) ;
  • 김홍식 (충북대학교 농업생명환경대학)
  • Received : 2018.01.01
  • Accepted : 2018.02.08
  • Published : 2018.03.01
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Abstract

This study was carried out to investigate the variation of 12 isoflavone components among 172 Korean soybean varieties released from 1913 to 2013. Cluster analysis was performed to classify the varieties based on the variation of isoflavone contents. Total isoflavone contents ranged from $206.3{\mu}g/g$ to $2666.2{\mu}g/g$ with an average of $837.2{\mu}g/g$. By each component, malonylgenistin content ranged from $99.2{\mu}g/g$ to $1149.1{\mu}g/g$ with the highest average of $390.0{\mu}g/g$, and glycitein content ranged from $0.1{\mu}g/g$ to $2.4{\mu}g/g$ with the lowest average of $0.8{\mu}g/g$. Among composition groups, the aglycone group contents ranged from $2.2{\mu}g/g$ to $33.1{\mu}g/g$ with the lowest average of $10.0{\mu}g/g$, and glucoside group contents ranged from $36.9{\mu}g/g$ to $801.8{\mu}g/g$ with an average of $202.4{\mu}g/g$. The malonylglucoside group contents ranged from $143.6{\mu}g/g$ to $1796.4{\mu}g/g$ with the highest average of $599.1{\mu}g/g$ and acetylglucoside group contents ranged from $3.7{\mu}g/g$ to $103.9{\mu}g/g$ with an average of $27.5{\mu}g/g$. Varieties with high total isoflavone content were Aga8($2666.2{\mu}g/g$), Aga4($2569.3{\mu}g/g$), and Aga9($2345.0{\mu}g/g$) in bean sprouts group. The low total isoflavone content were observed from Deawang($328.8{\mu}g/g$) in soy sauce and paste group, Hanol($599.3{\mu}g/g$) in vegetable and early maturity group and Gemjeong1($664.1{\mu}g/g$) in cooking with rice group. The first three principal component axises accounted for 89.14% of the total variation observed. Dendrogram constructed using average linkage cluster method revealed that 172 Korean soybean varieties were divided into seven groups with the average distance of 0.8 between groups. The group I includes 57.6% of the total varieties, which was the largest group among seven groups, followed by the group II that includes 35.5% of the total varieties. However, the other groups were small groups containing less than five varieties. In comparison among the total isoflavone contents according to groups, the highest isoflavone content was observed in group VI with an average of $2526.9{\mu}g/g$, followed by group IV($1546.6{\mu}g/g$) and group V($1503.5{\mu}g/g$). The others were found to contain low isoflavone content.

1913년부터 2013년까지 국내에서 육성된 장류용, 나물용, 풋콩 및 올콩용, 밥밑용 품종을 포함한 172 품종에 대한 종실의 isoflavone 12개 성분의 함량에 대한 변이를 구명하고, 다변량분석에 의한 품종군을 분류하였다. 종실의 총 isoflavone의 평균함량은 $837.2{\mu}g/g$이었고, $206.3{\sim}2666.2{\mu}g/g$의 범위이었다. 성분별로는 malonylgenistin의 평균함량이 $390.0{\mu}g/g$로서 가장 높았고, $99.2{\sim}1149.1{\mu}g/g$의 범위이었고, glycitein의 평균함량이 $0.8{\mu}g/g$로서 가장 낮았으며 $0.1{\sim}2.4{\mu}g/g$의 범위이었다. 성분그룹별로 보면 malonylglucoside group의 평균함량은 $599.1{\mu}g/g$로서 가장 높았고 $143.6{\sim}1796.4{\mu}g/g$의 범위이었으며, glucoside group의 평균함량은 $202.4{\mu}g/g$이었고 $36.9{\sim}801.8{\mu}g/g$의 범위이었다. acetylglucoside group의 평균함량은 $27.5{\mu}g/g$이었고 $3.7{\sim}103.9{\mu}g/g$의 범위이었으며, aglycone group의 평균함량은 $10.0{\mu}g/g$로서 가장 낮았고 $2.1{\sim}33.1{\mu}g/g$의 범위이었다. 총 isoflavone함량이 높은 품종은 나물용 품종인 아가8호($2666.2{\mu}g/g$), 아가4호($2569.3{\mu}g/g$) 및 아가9호($2345.0{\mu}g/g$)이었고, 총 isoflavone 함량이 낮은 품종은 장류용 품종인 대왕($328.8{\mu}g/g$), 풋콩 및 올콩 품종인 한올($599.3{\mu}g/g$) 그리고 밥밑용 품종인 검정콩1호($664.1{\mu}g/g$)이었다. Isoflavone성분에 대한 주성분분석을 실시한 결과, 제 1~3주성분까지의 누적기여도가 89.14%이었고, 제 3주성분만으로도 한국 콩 품종의 군집분류가 가능하였다. 군집분류에서 평균거리를 0.8로 하였을 때 7개의 군집으로 분류되었고, I군집은 전체 품종의 57.6%가 속하는 가장 큰 군집이었고, 다음으로 III군집이 35.5%가 속하는 큰 군집이었으며, 나머지 5개 군집은 5개 이하의 품종이 속하는 소군집이었다. 군집에 따른 총 isoflavone의 평균함량은 VI군집이 $2526.9{\mu}g/g$으로 가장 높았으며, 다음으로 IV군집이 $1546.6{\mu}g/g$, V군집이 $1503.5{\mu}g/g$으로 높았으며, 그 외 군집은 낮았다.

Keywords

Acknowledgement

Supported by : 충북대학교

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