Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantification, Anti-inflammatory and Antioxidant Effects of Mahwang-tang Decoction According to the Storage Temperature and Period

보관 온도 및 기간에 따른 마황탕 전탕액의 지표 성분 함량, 항염증 및 항산화 활성 비교

  • Lee, Nari (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hyekyung (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 이나리 (한국한의학연구원 한약연구부) ;
  • 하혜경 (한국한의학연구원 한약연구부) ;
  • 신현규 (한국한의학연구원 한약연구부) ;
  • 서창섭 (한국한의학연구원 한약연구부)
  • Received : 2018.06.11
  • Accepted : 2018.06.26
  • Published : 2018.06.30
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Abstract

This study was designed to investigate the stability for chemical contents and biological activities of Mahwang-tang (MT) depending on the preservation temperature and periods. MT decoction pouches were preserved for 3 months at room temperature (RT, $23{\pm}1^{\circ}C$) and refrigeration ($4^{\circ}C$). To evaluate the stability of MT decoction, pH and sugar content were estimated. High-performance liquid chromatography analysis were performed to quantify the contents of marker compounds in MT. Anti-inflammatory effects of MT were evaluated to suppress the generation of nitric oxide, prostaglandin $E_2$ and cytokines (tumor necrosis $factor-{\alpha}$ and interleukin-6) in the RAW 264.7 cells stimulated with lipopolysaccharide. Total antioxidant capacity of MT was determined by ABTS radical scavenging activity. The pH of storage method and period showed a tendency to decrease gradually with time. There were no changes in sugar content depending on the preservation temperature and periods of MT decoction. Among the major components of MT, cinnamaldehyde was reduced time-dependently for 3 months of storage at RT. The inflammatory effect and antioxidant capacity of MT were reduced time-dependently at both RT and $4^{\circ}C$. Our results suggest that the preservation period of MT decoction is recommended in refrigeration within 3 months or less rather than at RT.

Keywords

References

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