Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Evaluation of the Discoloration of Pyropia yezoensis Ueda Using a Colorimeter

색차계를 이용한 방사무늬김(Pyropia yezoensis Ueda)의 황백화 평가

  • Lee, Sang Yong (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Kim, Young Hee (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Lee, Jee Eun (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Hwang, Eun Kyoung (Seaweed Research Center, National Institute of Fisheries Science)
  • 이상용 (국립수산과학원 해조류연구센터) ;
  • 김영희 (국립수산과학원 해조류연구센터) ;
  • 이지은 (국립수산과학원 해조류연구센터) ;
  • 황은경 (국립수산과학원 해조류연구센터)
  • Received : 2018.11.26
  • Accepted : 2018.12.14
  • Published : 2018.12.31
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Abstract

Pyropia yezoensis is one of the most extensively cultivated seaweeds used as food in Korea. However, the quality and yield of Pyropia farms are frequently affected by outbreaks of thallus discoloration. The objective of this study was to develop a simple and rapid method to evaluate P. yezoensis discoloration caused by nutrient deficiencies. A colorimeter was used to quantify the color of P. yezoensis in nutrient deficient media over 10 days. Quantitative values of lightness (L), redness (a), and yellowness (b) measured with a colorimeter were used as indicators of P. yezoensis discoloration. The vacuoles of P. yezoensis were observed at 4 days and swelled gradually from 6 days onward. The three colorimeter parameters L, a, and b values showed significantly different values with culture period. The lightness (L) value was darker initially, and continued to lighten over culture period. The yellowness (b) value increased, but the redness (a) value decreased throughout this study. The a/b ratio and redness (a) values from the colorimeter were important P. yezoensis color change indicators. The discoloration group of P. yezoensis had quantitative values for parameters of L above 70, a below 2.6, and b above 15.0. The results of this study showed that quantitative colorimetric measurements can be used as tools for simple and rapid detection of P. yezoensis discoloration.

김은 한국에서 가장 경제성이 높은 양식 해조류이다. 그러나 최근 김 양식장에서 발생하는 황백화 현상으로 인해 엽체의 변색과 탈락이 자주 유발되어 수산업에 큰 피해를 유발하고 있다. 이 연구는 영양염 부족에 의한 김 황백화를 간단하고 빠르게 평가할 수 있는 방법을 개발하기 위한 목적으로 수행되었다. 10일 동안 빈영양 해수에서 배양한 방사무늬김 엽체의 색도를 정량화하기 위해 색차계를 사용하였다. 색차계로 측정한 명도(L), 적색강도(a)와 노란색강도(b) 값들로 방사무늬김의 황백화를 평가하였다. 방사무늬김 엽체의 액포는 4일차부터 관측되었으며, 6일차부터 액포가 증대하였다. 색차계의 L, a와 b값은 배양시간에 따라 유의한 차이를 보였다. 명도(L)는 시간의 경과에 따라 어두움에서 점차 밝아졌다. 노란색강도 (b) 값은 시간에 따라 증가하였으나, 적색강도(a) 값은 감소하였다. 색차계의 a/b 비와 적색강도(a) 값은 방사무늬김의 색도 차이를 구분하는 주요한 요인이었다. 황백화된 방사무늬김은 명도 (L) 70 이상, 적색강도(a) 2.6 이하와 노란색강도(b) 15.0 이상의 정량적인 값으로 구분되었다. 이 연구결과 색차계는 김 황백화의 정도를 손쉽고 빠르게 진단할 수 있는 유용한 도구로 이용할 수 있음을 밝혔다.

Keywords

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