Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Thirteen Weeks Repeated-dose Toxicity Study on Aconitum ciliare Decaisne Pharmacopuncture Solution in Mice

초오 약침액의 13주 반복 시술 독성에 관한 연구

  • Lim, Sung Chul (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Bong Hyo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Hyun Jong (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Hyun (Departmnet of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University) ;
  • Lee, Yun Kyu (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University)
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현 (대전대학교 한의과대학 침구의학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실)
  • Received : 2018.08.27
  • Accepted : 2018.09.19
  • Published : 2018.09.27
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Abstract

Objectives : This study was performed to examine the toxicity on the long term procedure of Aconitum ciliare Decaisne pharmacopuncture(ADP) solution. Methods : To evaluate the long term toxicity of 3 different repeated doses, 60, 150, and 300 mg/kg/day for 13 weeks were injected into BALB/c mice, respectively. The ADP solution was injected into near ST36 of the right leg and normal saline of the same volume was used for the vehicle control group. To evaluate the toxicity of 60, 150, and 300 mg/kg of repeated doses for 13 weeks, toxic symptoms, weight measurement, hematological test, blood biochemical test, visual examination and weight measurement of major organs, and histopathological test were conducted. Results : No significant changes in toxic symptoms, weight measurement, hematological test, blood biochemical test, visual examination and weight measurement of major organs, and histopathological test were observed in different doses of ADP solution treated groups compared to vehicle control group. Conclusions : As a result, repeated dose at a concentration of 300 mg/kg or less is considered to be not harmful for clinical treatment.

Keywords

References

  1. Lee DH. The study on Experimental Protocols for Safety Evaluation of Pediatric Drugs. Kyung Hee University Graduate School. 2009.
  2. Jang IS, Yang CH, Lee SD. A Review of Herbal Medicinal Products Associated with Toxic Events in Korea. J. of Korean Med. 2007 ; 28(1) : 1-10.
  3. Yang KR, Song SH. Effect of Snake Venom Toxin from Vipera Lebetina turanica on Breast Cancer Cells. J. of Acupuncture Res. 2009 ; 26(3) : 27-38.
  4. Oh MJ, Song HS. Inhibitory Effect of Snake Venom on Colon Cancer Cell Growth Through Induction of Death Receptor Dependent Apoptosis. J. of Acupuncture Res. 2012 ; 29(1) : 25-35.
  5. Lee BC, Song HS. Effect of Snake Venom on Cancer Growth through Induction of Apoptosis via Down Regulation of NF-$\kappa$B and STAT3 in the PA-1, Ovarian Cancer Cells. J. of Acupuncture Res. 2012 ; 29(1) : 37-45.
  6. Kim DH, Kim HW, Lee GH, Lee GM. Effect of Scolopendrid Pharmacupuncture Therapy on Frozen Shoulder Patients. J. of Acupuncture Res. 2009 ; 26(1) : 1-14.
  7. Kwak KI, Kang JH, Yoon KS, Cho E, Lee H. The Effect of Scolopendrid Pharmacopuncture on Postauricular Pain as an Early Symptom of Bells's Palsy. J. of Acupuncture Res. 2012 ; 29(5) : 51-9.
  8. Ku JY, Lee KH, Cho SW, Lee SC, Youn HM, Jang KJ, et al. Comparison of the Effects between Sweet Bee Venom Pharmacopuncture and Scolopendrid Pharmacopuncture on Carpal Tunnel Syndrome (Randomized, Controlled Clinical Trial. J. of Pharmacopuncture. 2010 ; 13(4) : 75-89. https://doi.org/10.3831/KPI.2010.13.4.075
  9. Lee YH, Kim CW, Lee KH. A Case Report of Patients with Calcific Tendinitis Located at Supraspinatus Treated by Oriental Therapy Including Sweet Bee Venom. J. of Acupuncture Res. 2012 ; 29(2) : 89-97.
  10. Han KW, Kim ES, Woo JH, Lee SJ, Lee JS, Nam JH, et al. Clinical Observation on 119 Patients with Lumbar Spinal Stenosis Treated with Bee Venom Pharmacopuncture Therapy. J. of Acupuncture Res. 2011 ; 28(3) : 21-31.
  11. An BJ, Kim KT, Kang MS, Song HS. Effect of Bee Venom-acupuncture on Patients with Osteoarthritis of Knee Joint. J. of Acupuncture Res. 2009 ; 23(4) : 15-20.
  12. Shin MK. Clinical traditional herbalogy. Seoul. youngrimsa. 1997 : 302-4.
  13. Kim SC, Kim YS, Ha YR, Kim HJ. An Outbreak of Caowu-intoxication after Drinking Home-brewed Alcohol . J. of the Kor Soc. of Emergency Med. 2008 ; 19(3) : 339-45.
  14. Hyun SC, Kim SR, Park SM, Lee H, Kim KD, Song WS. Case of Vetricular Tachycardia after Caowu-Ingestion. J. of the Kor Soc. of Emergency Med. 1997 ; 8(3) : 434-40.
  15. Kim SC, Ahn SH, Kim SH, Lee SK, Song BK. The Study on toxicity and biological activities of Aconiti ciliare tuber Pharmacopuncture in Rats. J. of Pharmacopuncture. 2011 ; 14(1) : 25-34. https://doi.org/10.3831/KPI.2011.14.1.025
  16. Jeong HR,Son BW, Kim GC, Shin SS, Hwang WD. Single Oral Dose Toxicity Study and Aconitine Content Analysis of Raw Aconiti Tuber and Sambu-tang. Kor J. Orient. Int. Med. 2016 ; 37(3) : 427-44.
  17. Kim YK, Lee JH, Song KY, Park SK, Kim CS Studies on the Liver Toxicities with different Dosage of Wild Aconiti Tuber Decoction . Herbal Formula Science. 2005 ; 13(1) : 123-43.
  18. Bang SP, Ryu MS, Kim JH, Wei TS, Yun DH, Yun YC et al. Effects of Rad III Aconiti Herbal Acupuncture Injected at Hwando(GB30) on Neuropathic Pain in Rats. J. of Acupuncture Res. 2009 ; 26(6) : 67-76.
  19. Lee HS, Lee YK, Lee BH, Kim JS, Lim SC, Lee HJ. An Experimental Study on Single-dose and 4 Weeks Repeated-dose Toxicity of Aconitum ciliare Decaisne Pharmacopuncture. Kor J. of Acupuncture. 2017 ; 34(4) : 241-50. https://doi.org/10.14406/acu.2017.027
  20. Korean Acupuncture and Moxibustion Medicine Society Textbook Compilation Committee. Acupuncture(sang). Paju : Jibmoondang. 2008 : 63-5.
  21. Youn SM, Lim JK. Acute and Subacute Toxicity Study of Artemisia asistica Nakai Aqua-acupuncture Solution in Mice. J. of Acupuncture Res. 2000 ; 17(1) : 143-51.
  22. Jung SY, Kim GY, Kim BH, Jo YB, Toxicology. Seoul. Donghwa Technology. 2000 : 15-21. 118-94.
  23. KO YS, Yun SH, Kim MK, Sonh YS, Shin BC. Literatural Study on Toxicity of Herbs used in . Kor J. of Oriental Physiology & Pathology. 2005 ; 19(5) : 1120-8.
  24. Lee JM, Jang WS, Seo BI. A philological study on poisoning of Aconiti radix. The J. of Applied Oriental Medicine. 2009 ; 9(1) : 23-41.

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