Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Histological Evaluation of Bioresorbable Threads in Rats

랫드에서의 생분해성 매선요법에 대한 조직학적 분석

  • Lee, Chang Gun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Jung, Jaeyun (Mielle Dermatology & Plastic Surgery Network Clinic) ;
  • Hwang, Samnoh (Department of Pathology, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Park, Chan Oh (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Hwang, Soonjae (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Jo, Minjeong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Sin, Min Hi (Department of Pathology, Wonju Severance Christian Hospital) ;
  • Kim, Hyun Ho (NEO Dr. Inc.) ;
  • Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • 이창근 (연세대학교 보건과학대학 임상병리학과) ;
  • 정재윤 (미엘르의원) ;
  • 황삼노 (가톨릭대학교 서울성모병원 병리과) ;
  • 박찬오 (연세대학교 보건과학대학 임상병리학과) ;
  • 황순재 (연세대학교 보건과학대학 임상병리학과) ;
  • 조민정 (연세대학교 보건과학대학 임상병리학과) ;
  • 신민희 (원주세브란스기독병원 병리과) ;
  • 김현호 ((주)네오닥터) ;
  • 이기종 (연세대학교 보건과학대학 임상병리학과)
  • Received : 2018.08.14
  • Accepted : 2018.09.03
  • Published : 2018.09.30
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Abstract

Thread lifting has become popular as a minimally invasive technique for facial rejuvenation. Commercially available threads are composed of poly-L-lactic acid (PLLA), polycaprolactone (PCL), or polydioxanone (PDO). However, the histological changes that occur in response to implanted threads are unclear. The aim of this study was to evaluate histological changes that occur in response to implantation with three types of bioresorbable threads (PLLA, PCL, PDO) in rat skin. PLLA, PCL and PDO threads were implanted in the dorsal skin of Sprague Dawley rats and tissue samples were harvested 2, 4, 8 and 12 weeks post-implantation. To evaluate histologic changes induced by bioresorbable face-lifting threads, tissue samples were stained with hematoxylin & eosin, Masson's trichrome stain and Herovici's collagen stain. All three threads induced neocollagenesis of type 3 collagen in the rat skin. The amount of collagen induced by the threads was dependent on the thread surface area. The PDO cavern-type thread was most effective in inducing neocollagenesis due to its extensive surface area. Our results suggest that type 3 collagen induced by bioresorbable threads depends on the thread surface area to uphold the dermis and contributes to facial rejuvenation.

매선요법은 다른 시술에 비해 비침습적이고, 인체 내에서 생분해가 일어나는 물질로 제작되기 때문에 성형수술에서 각광받고 있다. 상업적으로 이용가능한 매선의 종류로는 poly-L-lactic acid (PLLA), polycarprolactone (PCL), polydioxanone (PDO) 등이 있으나, 매선으로 사용되는 생분해성 물질들의 종류에 따른 차이점이나, 콜라겐을 형성하는데 있어서 매선의 어떤 부분이 결정적으로 작용하는지에 대한 연구는 잘 알려져 있지 않다. 따라서 이 연구에서는 현재 판매되고 있는 3가지 생분해성 물질(PLLA, PCL, PDO)로 만들어진 매선을 동물모델에서 그 효과를 비교하고, 매선의 콜라겐생성능에 미치는 영향 인자가 무엇인지를 분석하였다. 매선을 랫드의 등에 삽입하여 2주, 4주, 8주, 12주동안 경과를 관찰하고 특수염색을 통해 콜라겐의 생성 정도를 비교하였다. 매선에 의한 조직학적 변화를 관찰하기 위해서, 조직검체는 H&E, Masson's trichrome, Herovici's collagen stain이 진행되었다. 그 결과 3가지 모두 다 동일한 시기에 비슷한 양의 신생콜라겐을 형성하는 것을 관찰할 수 있었고, 3가지 매선 모두 type 3의 콜라겐을 형성하는 것을 확인할 수 있었다. 또한 생성되는 콜라겐의 양은 매선이 피부조직에 닿는 면적이 크면 클수록 증가하는 것을 관찰할 수 있어 한 번의 시술만으로도 많은 양의 매선이 들어갈 수 있는 PDO cavern타입의 매선이 가장 많은 콜라겐을 형성하는 것을 확인할 수 있었다.

Keywords

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