Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Influences of Insect-Resistant Genetically Modified Rice (Bt-T) on the Diversity of Non-Target Insects in an LMO Quarantine Field

LMO 격리 포장에서 해충저항성벼(Bt-T)가 비표적 곤충다양성에 미치는 영향

  • Oh, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA) ;
  • Park, Soo-Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA) ;
  • Chang, Ancheol (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA) ;
  • Lim, Myung-ho (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA) ;
  • Park, Soon Ki (School of Applied Biosciences, Kyungpook National University) ;
  • Suh, Sang Jae (School of Applied Biosciences, Kyungpook National University)
  • 오성덕 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 박수윤 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 장안철 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 임명호 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 박순기 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 서상재 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2018.09.13
  • Accepted : 2018.11.02
  • Published : 2018.12.01
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Abstract

This study was conducted to develop environmental risk assessments and biosafety guides for insect-resistant genetically modified rice in an LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-T), rice resistant to Cnaphalocrocis medinalis, and non-GM rice (Dongjin-byeo and Ilmi-byeo) were investigated. The Bt-T plants were, therefore, evaluated under field conditions to detect possible impacts on above ground insects and spiders. In 2016 and 2017, the study compared transgenic rice and two non-GM reference rice, namely Dongjin-byeo and Ilmi-byeo, at Gunwi. A total of 9,552 individuals from 51 families and 11 orders were collected from the LMO isolation field. From the three types of rice fields, a total of 3,042; 3,212; and 3,297 individuals from the Bt-T, Dongjin-byeo, and Ilmi-byeo were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies, and other insects on the Bt-T compared to non-GM rice. The data on insect species population densities were subjected to principal component analysis (PCA) without distinguishing between the three varieties, namely GM, non-GM, and reference cultivar, in all cultivation years. However, the PCA clearly separated the samples based on the cultivation years. These results suggest that insect species diversities and population densities during plant cultivation are determined by environmental factors (growing condition and seasons) rather than by genetic factors.

본 연구는 토양 세균인 B. thuringiensis에서 유래한 해충저항성 유전자(cry1Ac)를 일미벼에 도입시킴으로써 혹명나방(Cnaphalocrocis medinalis)에 대하여 살충성을 나타내는 해충저항성 Bt벼(Bt-T)의 환경위해성 평가에 대한 프로토콜 및 가이드라인을 개발하고자 수행하였다. 경북대학교 군위 LMO 격리포장에서 2년간 해충저항성 Bt벼(Bt-T)와 모품종인 동진벼 및 재배 품종인 일미벼를 재배하고, 혹명나방 등 표적곤충을 제외한 곤충류와 거미류가 포함한 다양성을 조사를 2016년과 2017년에 수행하였다. 조사기간 동안 채집된 개체들은 벼에 대한 기능별로 해충군, 천적군, 기타 곤충군으로 크게 구분하여 동정 계수하였으며, 2년간 총 11목 51과 9,552개체가 채집되었다. 해충저항성 Bt벼(Bt-T), 동진벼 및 일미벼에서 각각 3,042, 3,213, 3,297개체가 채집되었다. 조사된 개체군의 해충군, 천적군, 기타 곤충의 발생량은 해충군에서 노린목을 제외하고 해충저항성 Bt벼(Bt-T)과 모품종인 동진벼와 재배품종인 일미벼 품종 간의 각 분류군별 발생에서 통계적인 유의차가 없었으며, 시기별 곤충 발생 양상에서도 통계적인 유의적 차이를 보이지 않았다. 2년간의 곤충상 데이터를 주성분 분석법으로 분석한 결과, 2016년과 2017년 각각에서 동일하게 해충저항성 Bt벼(Bt-T)와 동진벼 및 일미벼 품종간의 곤충상 차이를 보이지 않았으나, 재배 시기에 따라 확연한 차이가 있음을 확인하였다. 유전자 변형에 의한 품종간의 차이 보다는 작물의 재배시기에 따라 곤충상 변화에 큰 영향을 끼치는 요인임을 새롭게 확인 할 수 있었다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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