Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Construction of Improved PCR Primer Set for the Detection of Human Enteric Adenovirus 41

  • Cho, Kyu-Bong (Department of Biomedical Laboratory Science, Shinhan University)
  • Received : 2018.08.17
  • Accepted : 2018.09.11
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Human enteric Adenovirus-41 (HuEAdV-41) causes gastroenteritis, which detected by the polymerase chain reaction (PCR) base diagnostic system for clinical, food, environmental, fish and shellfish samples. We developed improved PCR and nested PCR primer set which had high specificity, sensitivity and reduced times. In this study, we compared seventeen conditions reported in the previous study that was using the PCR based HuEAdV-41 detection system, and non-enteric Adenovirus were detected in nine conditions. The most sensitive detection condition was up to 25 copies however it took 184 minutes of PCR reaction time. In this study, the PCR primer set developed had same level of sensitivity, it reduced the time of detection for clinical, food and seafood samples to 112 minutes. Developed nested PCR primer set needed 112 minutes but detected up to approximately 1 copy. In addition, developed PCR and nested PCR primer set was validated with twenty samples of underground water at random, of which ten samples showed specific band without non-specific reaction. We expect this study will be used to diagnose HuEAdV-41 from various samples.

Keywords

References

  1. Akalu A, Seidel W, Liebermann H, Bauer U, Dohner L. Rapid identification of subgenera of human Adenovirus by serological and PCR assays. J Virol Methods. 1998. 71: 187-196. https://doi.org/10.1016/S0166-0934(97)00213-9
  2. Allard A, Albinsson B, Wadell G. Rapid typing of human Adenoviruses by a general PCR combined with restriction endonuclease analysis. J Clin Microbiol. 2001. 39: 498-505. https://doi.org/10.1128/JCM.39.2.498-505.2001
  3. Allard A, Albinsson B, Wadell G. Detection of Adenoviruses in stools from healthy persons and patients with diarrhea by twostep polymerase chain reaction. J Med Virol. 1992. 37: 149-157. https://doi.org/10.1002/jmv.1890370214
  4. Arcangeletti MC, Germini D, Martorana D, Rodighiero I, Conto FD, Medici MC, Chezzi C, Calderaro A. High frequency of cultivable human subgroup F Adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis. J Med Virol. 2014. 63: 812-818.
  5. Castignolles N, Petit F, Mendel I, Simon L, Cattolico L, Buffet-Janvresse C. Detection of Adenovirus in the waters of the seine river estuary by nested-PCR. Mol Cell Probes. 1998. 12: 175-180. https://doi.org/10.1006/mcpr.1998.0166
  6. Echavarria M, Forman M, Ticehurst J, Dumler JS, Charache P. PCR method for detection of Adenovirus in urine of healthy and human immunodeficiency virus-infected individuals. J Clin Microbiol. 1998. 36: 3323-3326.
  7. Flomenberg P, Piaskowski V, Truitt RL, Casper JT. Characterization of human proliferative T cell responses to Adenovirus. J Infect Dis. 1995. 171: 1090-1096. https://doi.org/10.1093/infdis/171.5.1090
  8. Grimwood K, Carzino R, Barnes GL, Bishop RF. Patients with enteric Adenovirus gastroenteritis admitted to an australian pediatric teaching hospital from 1981 to 1992. J Clin Microbiol. 1995. 33: 131-136.
  9. Girones G, Puig M, Allard A, Lucena F, Wadell G, Jofre J. Detection of Adenovirus and enterovirus by PCR amplification in polluted waters. Wal Sci Tech. 1995. 31: 351-357.
  10. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser. 1999. 41: 95-98.
  11. Hansman GS, Oka T, Li TC, Nishio O, Noda M, Takeda N. Detection of human enteric viruses in Japanese clams. J Food Prot. 2008. 71: 1689-1695. https://doi.org/10.4315/0362-028X-71.8.1689
  12. Jang J, Lee YJ, Kim JS, Chung JY, Chang S, Lee K, Choe BH, Hong SJ, Song JS, Park KY. Epidemiological correlation between fecal Adenovirus subgroups and pediatric intussusception in Korea. J Korean Med Sci. 2017. 32: 1647-1656. https://doi.org/10.3346/jkms.2017.32.10.1647
  13. Johansson ME, Uhnoo IMD, Svensson L, Pettersson CA, Wadell G. Enzyme-linked immunosorbent assay for detection of enteric Adenovirus 41. J Med Virol. 1985. 17: 19-27. https://doi.org/10.1002/jmv.1890170104
  14. Jo YK, Lee CY. Detection of Salmonella typhi by loop-mediated isothermal amplification assay. J Exp Biomed Sci. 2008. 14: 115-118.
  15. Kidd AH, Jonsson M, Garwicz D, Kajon AE, Wermenbol AG, Verweij MW, Jong JC. Rapid subgenus identification of human Adenovirus isolates by a general PCR. J Clin Microbiol. 1996. 34: 622-627.
  16. Kibbe WA. OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Res. 2007. 35: 43-46.
  17. Kim YS, Kim JB, Eom YB. Detection of pathogenic Yersinia enterocolitica strains by a rapid and specific multiplex PCR assay. J Exp Biomed Sci. 2004. 10: 333-339.
  18. Korea Ministry of Food and Drug Safety. The statistics for reported foodborne outbreaks in Korea. 2013. Available at: http://www.KMFDS.go.kr/e-stat/index.do, accessed Jun 30, 2018.
  19. Kuo DH, Simmons F, Xagoraraki I. A new set of PCR assays for the identification of multiple human Adenovirus species in environmental samples. J Appl Microbiol. 2009. 107: 1219-1229. https://doi.org/10.1111/j.1365-2672.2009.04300.x
  20. Munkhdelger J, Mia-Jan K, Lee D, Park S, Kim S, Choi Y, Wang HY, Jeon BY, Lee H, Park KH. Performance of quantitative real-time PCR for detection of tuberculosis in Granulomatous Lymphadenitis using formalin-fixed paraffin-embedded tissue. J Exp Biomed Sci. 2013. 19: 153-157.
  21. Oh DY, Gaedicke G, Schreier E. Viral agents of acute gastroenteritis in German children: prevalence and molecular diversity. J Med Virol. 2003. 71: 82-93. https://doi.org/10.1002/jmv.10449
  22. Rowe WP, Huebner RJ, Gilmore LK, Parrott RH, Ward TG. Isolation of a cytopathogenic agent from human Adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med. 1953. 84: 570-573. https://doi.org/10.3181/00379727-84-20714
  23. Tai YF. The study of molecular biology method for the gastroenteritis syndrome in stool. National Digital Library of Theses and Dissertations in Taiwan. 2008. Masters dissertation.
  24. Uhnoo I, Wadell G, Svensson L, Johansson ME. Importance of enteric Adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol. 1984. 20: 365-372.
  25. Wang HY, Whang JH, Kim JP, Cho JE, Bang H, Lee H, Cho SN. Improved detection of Mycobacterium leprae by one-tube nested polymerase chain reaction. J Exp Biomed Sci. 2017. 16: 319-324.
  26. Xu W, Mcdonough MC, Erdman DD. Species-specific identification of human Adenoviruses by a multiplex PCR assay. J Clin Microbiol. 2000. 38: 4114-4120.