Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Fermentation-Mediated Enhancement of Ginseng's Anti-Allergic Activity against IgE-Mediated Passive Cutaneous Anaphylaxis In Vivo and In Vitro

  • Hwang, Seon-Weon (Graduate Program in Biomaterials Science and Engineering, Yonsei University) ;
  • Sun, Xiao (Department of Applied Life Science, Graduate School, Konkuk University) ;
  • Han, Jun-Hyuk (Department of Applied Life Science, Graduate School, Konkuk University) ;
  • Kim, Tae-Yeon (Department of Applied Life Science, Graduate School, Konkuk University) ;
  • Koppula, Sushruta (Department of Integrated Bioscience, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University) ;
  • Kang, Tae-Bong (Department of Integrated Bioscience, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University) ;
  • Hwang, Jae-Kwan (Graduate Program in Biomaterials Science and Engineering, Yonsei University) ;
  • Lee, Kwang-Ho (Department of Applied Life Science, Graduate School, Konkuk University)
  • Received : 2018.07.26
  • Accepted : 2018.08.18
  • Published : 2018.10.28
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Abstract

Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found to attenuate allergic responses in in vitro and in vivo experimental models. Ginseng has been reported to also possess various biological functions including anti-inflammatory activity. The present study was aimed at comparing the anti-allergic effect of ginseng and fermented ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line and in vivo in mice. Fermented ginseng extract (FPG) showed higher inhibitory effect against in vitro and in vivo allergic responses when compared with ginseng extract (PG). The secretion of ${\beta}$-hexosaminidase and interleukin (IL)-4 from the IgE-DNP-stimulated RBH-2H3 mast cells were significantly (p < 0.05) inhibited by FPG treatment, and this effect was concentration-dependent. Further, MKK4 activation and subsequent JNK phosphorylation were attenuated by FPG treatment. The inhibitory effect of FPG on the in vitro allergic response was verified in vivo against IgE-DNP-induced passive cutaneous anaphylaxis in a mouse model. These data indicated that the fermentation of ginseng with L. plantarum enhanced its anti-allergic effects both in vitro and in vivo. We predict that compositional changes in the ginsenosides caused by the fermentation may contribute to the change in the anti-allergic effects of ginseng. The results of our study highlight the potential of the use of FPG as a potential anti-allergic agent.

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References

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