한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제22권2호
  • /
  • Pages.193-198
  • /
  • 2018
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis

  • Yoon, Jong-Man (Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 투고 : 2018.04.10
  • 심사 : 2018.05.14
  • 발행 : 2018.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus, which was further associated with those of the other clam population, by engaging with the precisely designed oligonucleotide primer sets. Seven oligonucleotides primers were used producing a total of 639 counted bands in population A and 595 in population B, respectively, ranging in size of DNA fragments from larger than approximately 50 bp to less than 1,100 bp. Their primers generated 39 specific fragments (6.10%) in population A and 47 (7.90%) in population B, respectively Comparatively, individuals of one razor clam population were fairly related to that of the other clam population, as shown in the hierarchical dendrogram of Euclidean genetic distances. The analysis of genetic variation between razor clam populations could offer important statistics for fisheries and mariculture. Generally the results showed specific and/or conserved genetic loci between razor clam populations. Specific markers established by the author will be valuable for the genetic analysis, species protection and increase of razor clam individuals in coastal region of the Korean Peninsula.

키워드

참고문헌

  1. Callejas C, Ochando MD (1998) Identification of Spanish barbel species using the RAPD technique. J Fish Biol 53:208-215. https://doi.org/10.1111/j.1095-8649.1998.tb00121.x
  2. Chenyambuga SW, Hanotte O, Hirbo J, Watts PC, Kemp SJ, Kifaro GC, Gwakisa PS, Petersen PH, Rege JEO (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Aust J Anim Sci 17:445-452. https://doi.org/10.5713/ajas.2004.445
  3. Chung EY, Kim HJ, Kim JB, Lee CH (2006) Changes in biocgemical components of several tissues in Solen grandis, in relation to gonad developmental phases. Korean J Malacol 22:27-38.
  4. Chung EY, Lee TR, Kim HB (1986) Annual reproductive cycle of the Jackknife clams, Solen strictus and Solen grandis. J Korean Fish Soc 19:563-574.
  5. Han JS, Kim JB, Lee CH, Chung EY (2005) Changes in biochemical components of several tissues in Sinonovacula constricta, in relation to gonad developmental phases. Korean J Malacol 21:121-132.
  6. Huang BX, Peakall R, Hanna PJ (2000) Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Mar Biol 136:207-216. https://doi.org/10.1007/s002270050678
  7. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15.
  8. Kang SK, Yoon JM (2013) Geographic variations of three Fulvia mutica populations. Korean J Malacol 29:163-169. https://doi.org/10.9710/kjm.2013.29.3.163
  9. Kim SK, Jung YH, Han SH, Oh YS, Ko MH, Oh MY (2000) Phylogenetic relationship among Haliotis spp. distributed in Korea by the RAPD analysis. Korean J Genet 22:43-49.
  10. Klinbunga S, Boonyapakdee A, Pratoomchat B (2000). Genetic diversity and species-diagnostic markers of mud crabs (Genus Scylla) in Eastern Thailand determined by RAPD analysis. Mar Biotechnol 2:180-187.
  11. McCormack GP, Powell R, Keegan BF (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106.
  12. Muchmore ME, Moy GW, Swanson WJ, Vacquier VD (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar Biol Biotech 7:1-6.
  13. Oh H, Yoon JM (2014) Genetic distances of three mollusk species investigated by PCR analysis. Dev Reprod 18:43-49. https://doi.org/10.12717/DR.2014.18.1.043
  14. Park SY, Park JS, Yoon JM (2005) Genetic differences and variations in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) determined by RAPD analysis. Korean J Genet 25:307-317.
  15. Partis L, Wells RJ (1996) Identification of fish species using random amplified polymorphic DNA (RAPD). Mol Cell Probes 10:435-441. https://doi.org/10.1006/mcpr.1996.0060
  16. Song YJ, Yoon JM (2013) Genetic differences of three Pollicipes mitella population identified by PCR analysis. Dev Reprod 17:199-205. https://doi.org/10.12717/DR.2013.17.3.199
  17. Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotechnol 6:249-254.
  18. Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001
  19. Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Australas J Anim Sci 17:1053-1061. https://doi.org/10.5713/ajas.2004.1053
  20. Yoon JM, Park HY (2002) Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Australas J Anim Sci 15:470-476. https://doi.org/10.5713/ajas.2002.470
  21. Zhou L, Wang Y, Gui JF (2000) Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Block, based on detection of RAPD molecular markers. Cytogenet Cell Genet 88:133-139. https://doi.org/10.1159/000015506