Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Protective efficacy of formalin-inactivated Salmonella Gallinarum whole cells vaccine using mastoparan V1 as adjuvant against fowl typhoid

가금티푸스 예방을 위한 adjuvant로서 mastoparan V1을 사용한 포르말린-불활화 Salmonella Gallinarum 사균체 백신의 효능 평가

  • Moon, Ja-Young (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Kwak, Kil Han (Animal Health Institute of Jeollabukdo) ;
  • Ochirkhuyag, Enkhsaikhan (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Kim, Seon-Min (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Lee, Jun-Woo (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Jo, Young-Gyu (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Kim, Won-Kyong (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University) ;
  • Bang, Woo Young (National Institute of Biological Resources, Ministry of Environment) ;
  • Bae, Chang Hwan (National Institute of Biological Resources, Ministry of Environment) ;
  • Hur, Jin (Department of Veterinary Public Health, College of Veterinary Medicinee, Chonbuk National University)
  • 문자영 (전북대학교 수의과대학 수의공중보건학실) ;
  • 곽길환 (전라북도가축위생시험소) ;
  • ;
  • 김선민 (전북대학교 수의과대학 수의공중보건학실) ;
  • 이준우 (전북대학교 수의과대학 수의공중보건학실) ;
  • 조영규 (전북대학교 수의과대학 수의공중보건학실) ;
  • 김원경 (전북대학교 수의과대학 수의공중보건학실) ;
  • 방우영 (환경부 국립생물자원관) ;
  • 배창환 (환경부 국립생물자원관) ;
  • 허진 (전북대학교 수의과대학 수의공중보건학실)
  • Received : 2019.12.04
  • Accepted : 2019.12.24
  • Published : 2019.12.30
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Abstract

Mastoparan V1 was used as adjuvant of formalin-inactivated Salmonella Gallinarum whole cells vaccine against fowl typhoid in a chicken model. The 75 brown nick chickens were equally divided into 5 groups, and all chickens of each group were immunized at 6 weeks of age (0 WPPI; weeks prime post immunization), and at 9 weeks of age (3 WPPI) (except group B). Group A chickens were intramuscularly (IM) inoculated with 500 uL of sterile phosphate-buffered saline (PBS), and group B chickens were subcutaneously immunized with 0.2 ml containing 5×107 viable vaccine strain/bird. The chickens in groups C~E were IM inoculated with approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells, approximately 3×109 cells/0.5 mL of formalin-inactivated the S. Gallinarum whole cells with mastoparan V1 as adjuvant, and 0.5 mL of PBS, respectively. S. Gallinarum outer membrane proteins-specific serum IgG titers were considerably higher in groups B~D than in groups A and E. However, the levels of IFN-γ in groups B and D only than in groups A and E were significantly higher. Following oral challenge with virulent wild-type S. Gallinarum, no chicken in groups A (no challenge group) and B was dead, and only 30% of chickens in group D was dead. However, 70% of chickens in group C and all chickens in group E were dead after oral challenge. The results of this study demonstrated that IM immunization with approximately 3×109 of the formalin-inactivated S. Gallinarum whole cells containing mastoparan V1 induced robust antibody and cell-mediated immune responses in chickens. The whole cells also conferred protection against infection with wild-type S. Gallinarum.

Keywords

References

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