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Analysis of Genetic Diversity and Relationships of Korean Native Black Goat using Microsatellite Markers

초위성체 마커를 이용한 한국 재래 흑염소의 유전적 다양성 및 유연관계 분석

  • Received : 2019.08.30
  • Accepted : 2019.09.23
  • Published : 2019.09.30

Abstract

The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

Keywords

References

  1. Belkhir K, Borsa P, Chikhi L, Raufaste N and Bonhomme F. 1996-2004. GENETIX 4.05, logiciel sous windows TM pour la genetique des populations, Laboratoire Genome, Populations, Interactions, CNRS UMR 5171, Universite de Montpellier II, Montpellier. France.
  2. Botstein D, White RL, Skolnick M and Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331.
  3. Chung ER. 2002. Identification of Korean Native Goat meat using amplified fragment length polymorphism (AFLP) DNA markers. Korean J. Food Sci. Ani. Res. 22:301-309.
  4. FAO. 2011. Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. Rome. 9:74-75.
  5. Hassen H, Lababidi S, Rischkowsky B, Baum M and Tibbo M. 2012. Molecular characterization of Ethiopian indigenous goat populations. Trop. Anim. Health Prod. 44:1239-1246. https://doi.org/10.1007/s11250-011-0064-2
  6. Hoda A, Haka G, Dunner S, Obexer-Ruff G and Consortium E. 2011. Genetic diversity of albanian goat breeds based on microsatellite markers. Arch. Zootec. 60:607-615. https://doi.org/10.4321/S0004-05922011000300049
  7. Kim BK, Lee JH, Jung DJ, Cho KH, Hwang EG and Kim MS. 2010. Effects of feeding herb resources powder on meat quality and sensory properties in Korean Native Black Goat. Korean J. Food Sci. Ani. Res. 30:811-818. https://doi.org/10.5851/kosfa.2010.30.5.811
  8. Kim JH, Byun MJ, Ko YG, Kim SW, Do YJ, Kim MJ, Yoon SH and Choi SB. 2012. Phylogenetic analysis of Korean Native Goats based on the mitochondrial cytochrome b Gene. Journal of Animal Science and Technology. 54(4):241-246. https://doi.org/10.5187/JAST.2012.54.4.241
  9. Kim SW, Lee J, Kim K-W, Kim C-L, Jeon IS and Lee S-S. 2017. Effects of triladyl-egg yolk diluents on the viability of frozen Korean Black goat spermatozoa from cauda epididymis and electro-ejaculated semen. J. Emb. Trans. 32(3):235-241. https://doi.org/10.12750/JET.2017.32.3.235
  10. MacHugh DE and Bradley DG. 2001. Livestock genetic origins: goats buck the trend. Proc. Natl. Acad. Sci. USA 98:5382-5384. https://doi.org/10.1073/pnas.111163198
  11. Park SDE. 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. Thesis. University of Dublin.
  12. Peakall R and Smouse PE. 2006. GENALEX 6: Genetic analysis in excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6:288-295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
  13. Porter V. 1996. Goats of the world. Farming Press, Ipswich, UK.
  14. Rout PK, Joshi MB, Mandal A, Laloe D, Singh L and Thangaraj K. 2008. Microsatellite-based phylogeny of Indian domestic goats. BMC Genet. 9:11. https://doi.org/10.1186/1471-2156-9-11
  15. Seo JH, Lee YS, Jeon GJ and Kong HS. 2017. Molecular genetic evaluation of gorals (naemorhedus caudatus raddeanus) genetic resources using microsatellite markers, Journal of the Korean Data & Information Science Society. 28(5):1043-1053.
  16. Suh SW. 2014. Molecular genetic evaluation of Korean domestic animal genetic resources using microsatellite markers. Gyeongsang National University. VIII. p. 131.
  17. Suh SW, Byun MJ, Kim YS, Kim MJ, Choi SB, Ko YG, Kim DH, Lim HT and Kim JH. 2012. Analysis of genetic diversity and relationships of Korean Native Goat populations by microsatellite markers. J. Life Sci. 22(11):1493-1499. https://doi.org/10.5352/JLS.2012.22.11.1493
  18. Suh SW, Cho CY, Byun MJ, Choi SB, Kim YS, Kim MJ, Ko YG, Kim DH, Lim HT and Kim JH. 2014. Establishment of a microsatellite marker set for individual identification in goat. J. Agr. Life Sci. 48(3):157-164. https://doi.org/10.14397/jals.2014.48.3.157
  19. Suh SW, Cho CY, Kim YS, Byun MJ, Choi SB, Cho YM, Bae KH, Kim JH. 2015. Molecular Genetic Considerations of Jeju Black Cattle using Micrisatellite Markers. J. Agr. Life Sci. 49: 57-65.
  20. Yang DY. 2018. Study on the genetic polymorphism of Hanwoo using MS marker information. Hankyoung National University. I804:41039-200000018363.
  21. Zeder MA and Hesse B. 2000. The initial domestication of goats (Capra hircus) in the Zagros Mountain 10,000 years ago. Science 287:2254-2257. https://doi.org/10.1126/science.287.5461.2254