Journal of the Korean Society of Environmental Restoration Technology (한국환경복원기술학회지)

The Korea Society of Environmental Restoration Technology (한국환경복원기술학회)
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Possibility in identifying species composition of fish communities using the environmental DNA metabarcoding technique - with the preliminary results at urban ecological streams

환경DNA 메타바코딩 기술을 활용한 수생태계 어류종 군집조사의 가능성 - 도시 생태하천 초기분석 자료를 중심으로

  • Song, Young-Keun (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Kim, Jong-Hee (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Won, Su-Yeon (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Park, Chan (Dept. of Landscape Architecture, University of Seoul)
  • 송영근 (서울대학교 환경대학원 환경조경학과) ;
  • 김종희 (서울대학교 환경대학원 환경조경학과) ;
  • 원수연 (서울대학교 환경대학원 환경조경학과) ;
  • 박찬 (서울시립대학교 조경학과)
  • Received : 2019.11.21
  • Accepted : 2019.12.21
  • Published : 2019.12.31
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Abstract

This study aims to highlight the possibility in identifying species composition of fish communities using the environmental DNA (eDNA) metabarcoding technique, from both of the technical introduction and the pilot test at urban ecological streams. This new emerging survey technique using eDNA is getting popular in the world as a compensating way for the conventional field survey. However, the application to the domestic cases has yet to be studied. We attempted to use this technique for identifying fish species observed at four survey points in Hwangguji-chon, Suwon City. As a result, the detected number of species by eDNA sampled once in May was significantly matched with the total number of observed species in annual field surveys. Additionally eDNA results indicated the presence possibility of the unobserved species in field last year, even though the validation may be required. This survey technique seems to be more efficient and applicable to diverse situations of the fields and species, thereby needs to be studied further. We discussed the pros and cons of the application and summarized the research directions in future.

Keywords

References

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