Journal of The Korean Society of Clinical Toxicology (대한임상독성학회지)

Korean society of Clincal Toxicology (대한임상독성학회)
권호 표지
DOI QR코드

DOI QR Code

Application of Thallium Autometallography for Observation of Changes in Excitability of Rodent Brain following Acute Carbon Monoxide Intoxication

흰쥐에서 급성 일산화탄소 중독 후 뇌 흥분성 변화를 규명하기 위한 탈륨 Autometallography의 적용

  • Lee, Min Soo (Department of Emergency Medicine, Wonkwang University School of Medicine) ;
  • Yang, Seung Bum (Department of Medical Non-commissioned Officer, Wonkwang Health Science University) ;
  • Heo, Jun Ho (Department of Emergency Medicine, Wonkwang University School of Medicine)
  • 이민수 (원광대학교 의과대학 응급의학교실) ;
  • 양승범 (원광보건대학교 의무부사관과) ;
  • 허준호 (원광대학교 의과대학 응급의학교실)
  • Received : 2019.09.11
  • Accepted : 2019.09.17
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Thallium (TI+) autometallography is often used for the imaging of neuronal metabolic activity in the rodent brain under various pathophysiologic conditions. The purpose of this study was to apply a thallium autometallographic technique to observe changes in neuronal activity in the forebrain of rats following acute carbon monoxide (CO) intoxication. Methods: In order to induce acute CO intoxication, adult Sprague-Dawley rats were exposed to 1100 ppm of CO for 40 minutes, followed by 3000 ppm of CO for 20 minutes. Animals were sacrificed at 30 minutes and 5 days after induction of acute CO intoxication for thallium autometallography. Immunohistochemical staining and toluidine blue staining were performed to observe cellular damage in the forebrain following intoxication. Results: Acute CO intoxication resulted in significant reduction of TI+ uptake in major forebrain structures, including the cortex, hippocampus, thalamus, and striatum. In the cortex and hippocampal CA1 area, marked reduction of TI+ uptake was observed in the cell bodies and dendrites of pyramidal neurons at 30 minutes following acute CO intoxication. There was also strong uptake of TI+ in astrocytes in the hippocampal CA3 area following acute CO intoxication. However, there were no significant histological findings of cell death and no reduction of NeuN (+) neuronal populations in the cortex and hippocampus at 5 days after acute CO intoxication. Conclusion: The results of this study suggest that thallium autometallography can be a new and useful technique for imaging functional changes in neural activity of the forebrain structure following mild to moderate CO intoxication.

Keywords

References

  1. Rose JJ, Wang L. Carbon Monoxide Poisoning: Pathogenesis, Management, and Future Directions of Therapy. Am J Respir Crit Care Med 2017; 195:596-606. https://doi.org/10.1164/rccm.201606-1275CI
  2. Gorman D, Drewry A, Huang YL, et al. The clinical toxicology of carbon monoxide. Toxicology 2003; 187:25-38. https://doi.org/10.1016/S0300-483X(03)00005-2
  3. Raub JA, Mathieu-Nolf M, Hampson NB, et al. Carbon monoxide poisoning--a public health perspective. Toxicology 2000;145:1-14. https://doi.org/10.1016/S0300-483X(99)00217-6
  4. Lee JS, Song JH, Lim BS. Characteristics of suicide by carbon monoxide poisoning in Korea. J Korean Official Stat 2016; 21:57-83.
  5. Choi YR, Cha ES, Chang SS, et al. Suicide from carbon monoxide poisoning in South Korea: 2006-2012. J Affect Disord 2014; 167:322-5. https://doi.org/10.1016/j.jad.2014.06.026
  6. Cho Y, Effects of acute carbon monoxide poisoning on venous thromboembolism: a population-based study, in Department of Emergency Medicine. 2019, Hanyang University, Seoul. p. 1-2.
  7. Oh S, Choi SC. Acute carbon monoxide poisoning and delayed neurological sequelae: a potential neuroprotection bundle therapy. Neural Regen Res 2015; 10:36-8. https://doi.org/10.4103/1673-5374.150644
  8. Kim DM, Lee IH, Park JY, et al. Acute carbon monoxide poisoning: MR imaging findings with clinical correlation. Diagn Interv Imaging 2017; 98:299-306. https://doi.org/10.1016/j.diii.2016.10.004
  9. Prockop LD. Carbon monoxide brain toxicity: clinical, magnetic resonance imaging, magnetic resonance spectroscopy, and neuropsychological effects in 9 people. J Neuroimaging 2005; 15:144-9. https://doi.org/10.1111/j.1552-6569.2005.tb00299.x
  10. Penney DG. Acute carbon monoxide poisoning: animal models: a review. Toxicology 1990; 62:123-60. https://doi.org/10.1016/0300-483X(90)90106-Q
  11. Prockop LD, Chichkova RI. Carbon monoxide intoxication: an updated review. J Neurol Sci 2007; 262:122-30. https://doi.org/10.1016/j.jns.2007.06.037
  12. Bi M, Li Q, Guo D, et al. Sulphoraphane Improves Neuronal Mitochondrial Function in Brain Tissue in Acute Carbon Monoxide Poisoning Rats. Basic Clin Pharmacol Toxicol 2017; 120:541-49. https://doi.org/10.1111/bcpt.12728
  13. Goldschmidt J, Zuschratter W, Scheich H. High-resolution mapping of neuronal activity by thallium autometallography. Neuroimage 2004; 23:638-47. https://doi.org/10.1016/j.neuroimage.2004.05.023
  14. Stober F, Baldauf K, Ziabreva I, et al. Single-cell resolution mapping of neuronal damage in acute focal cerebral ischemia using thallium autometallography. J Cereb Blood Flow Metab 2014; 34:144-52. https://doi.org/10.1038/jcbfm.2013.177
  15. Patel RA, Beller GA. Prognostic role of single-photon emission computed tomography (SPECT) imaging in myocardial viability. Curr Opin Cardiol 2006; 21:457-63. https://doi.org/10.1097/01.hco.0000240582.83967.d8
  16. Evans PH. Free radicals in brain metabolism and pathology. Br Med Bull 1993; 49:577-87. https://doi.org/10.1093/oxfordjournals.bmb.a072632
  17. Ischiropoulos H, Beers MF, Ohnishi ST, et al. Nitric oxide production and perivascular nitration in brain after carbon monoxide poisoning in the rat. J Clin Invest 1996; 97:2260-7. https://doi.org/10.1172/jci118667
  18. Meilin S, Rogatsky GG, Thom SR, et al. Effects of carbon monoxide on the brain may be mediated by nitric oxide. J Appl Physiol (1985) 1996; 81:1078-83. https://doi.org/10.1152/jappl.1996.81.3.1078
  19. Novaes LS, Dos Santos NB, Dragunas G, et al. Repeated Restraint Stress Decreases Na,K-ATPase Activity via Oxidative and Nitrosative Damage in the Frontal Cortex of Rats. Neuroscience 2018; 393:273-83. https://doi.org/10.1016/j.neuroscience.2018.09.037
  20. Jaiswal SK, Siddiqi NJ, Sharma B. Carbofuran induced oxidative stress mediated alterations in Na(+)-K(+)-ATPase activity in rat brain: amelioration by vitamin E. J Biochem Mol Toxicol 2014; 28:320-7. https://doi.org/10.1002/jbt.21568
  21. Ostadal P, Elmoselhi AB, Zdobnicka I, et al. Role of oxidative stress in ischemia-reperfusion-induced changes in Na+,K(+)-ATPase isoform expression in rat heart. Antioxid Redox Signal 2004; 6:914-23. https://doi.org/10.1089/1523086041798024
  22. Ostadal P, Elmoselhi AB, Zdobnicka I, et al. Ischemiareperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart. Biochem Biophys Res Commun 2003;306:457-62. https://doi.org/10.1016/S0006-291X(03)00986-0
  23. Watanabe S, Matsuo H, Kobayashi Y, et al. Transient degradation of myelin basic protein in the rat hippocampus following acute carbon monoxide poisoning. Neurosci Res 2010; 68:232-40. https://doi.org/10.1016/j.neures.2010.07.2029
  24. Zhao N, Liang P, Zhuo X, et al. After Treatment with Methylene Blue is Effective against Delayed Encephalopathy after Acute Carbon Monoxide Poisoning. Basic Clin Pharmacol Toxicol 2018; 122:470-80. https://doi.org/10.1111/bcpt.12940
  25. Koroleva VI, Korolev OS, Mares V, et al. Hippocampal damage induced by carbon monoxide poisoning and spreading depression is alleviated by chronic treatment with brain derived polypeptides. Brain Res 1999; 816:618-27. https://doi.org/10.1016/S0006-8993(98)01246-3
  26. Watson ES, Jones AB, Ashfaq MK, et al. Spectrophotometric evaluation of carboxyhemoglobin in blood of mice after exposure to marijuana or tobacco smoke in a modified Walton horizontal smoke exposure machine. J Anal Toxicol 1987;11:19-23. https://doi.org/10.1093/jat/11.1.19
  27. Laas R, Igloffstein J, Meyerhoff S. Cerebral infarction due to carotid occlusion and carbon monoxide exposure. I. Pathophysiological and neuropathological investigations. J Neurol Neurosurg Psychiatry 1983; 46:756-67. https://doi.org/10.1136/jnnp.46.8.756
  28. Tabrizian K, Shahraki J, Bazzi M, et al. Neuro-Protective Effects of Resveratrol on Carbon Monoxide-Induced Toxicity in Male Rats. Phytother Res 2017; 31:1310-15. https://doi.org/10.1002/ptr.5855
  29. Brismar T, Collins VP, Kesselberg M. Thallium-201 uptake relates to membrane potential and potassium permeability in human glioma cells. Brain Res 1989; 500:30-6. https://doi.org/10.1016/0006-8993(89)90296-5
  30. Igase K, Oka Y, Ohta S, et al. Usefulness of thallium-201 single photon emission computed tomography to quantify the malignancy grade of brain tumors. Neurol Med Chir (Tokyo) 1996; 36:434-9. https://doi.org/10.2176/nmc.36.434