Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Sulgidduk containing pine needle juice on lipid metabolism in high fat-cholesterol diet induced dyslipidemic rats

이상지질혈증 동물 모델을 이용한 솔잎 착즙액 첨가 설기떡의 지질개선 효과

  • Lee, Yunjung (Department of Food and Nutrition, Kyungnam University) ;
  • Park, Jae-Hee (Department of Food and Nutrition, Kyungnam University) ;
  • Park, Eunju (Department of Food and Nutrition, Kyungnam University)
  • 이윤정 (경남대학교 식품영양학과) ;
  • 박재희 (경남대학교 식품영양학과) ;
  • 박은주 (경남대학교 식품영양학과)
  • Received : 2019.01.03
  • Accepted : 2019.01.30
  • Published : 2019.02.28
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Abstract

Purpose: Dyslipidemia is a major risk factor for cardiovascular disease. Pine needles (Pinus densiflora seib et Zucc) are a traditional medicine used to treat dyslipidemia in clinical settings. This study examined the potential effects of sulgidduk, a Korean traditional rice cake containing pine needle juice to protect against dyslipidemia induced by a high-fat/sugidduk diet in a rat model. Methods: Twenty one male Sprague-Dawley rats were divided randomly into three groups: normal control (NC), Sulgidduk diet (SD), Sulgidduk diet containing pine needle juice (PSD). The blood lipid levels, production of lipid peroxide in the plasma and liver, total cholesterol and triglyceride in the liver and feces, antioxidant enzyme activities in plasma and erythrocytes were measured to assess the effects of PSD on dyslipidemia. Results: A high-fat/Sulgidduk diet induced dyslipidemia, which was characterized by significantly altered lipid profiles in the plasma and liver. The food intake was similar in the three groups, but weight gain and food efficiency ratio (FER) were reduced significantly in the PSD group compared to those in the SD group. The level of total cholesterol, LDL-cholesterol and TBARS in the plasma showed tendencies to decrease in the PSD group compared to those in the SD group. The levels of high-fat/Sulgidduk diet-induced sterol regulatory element-binding protein 2 (SREBP2) gene expression were reduced significantly in the PSD group. The supplementation of PSD reduced the hepatic triglyceride and total cholesterol levels significantly, and enhanced the fecal excretion of triglyceride and hepatic antioxidant enzyme activities compared to the SD group. Conclusion: These results suggest that the addition of 0.4% pine needle juice to Sulgidduk may be an alternative snack to control dyslipidemia.

본 연구는 솔잎착즙액이 고지방식이로 이상지질혈증을 유도한 흰쥐의 지질대사에 미치는 영향을 분석하기 위해 실험동물을 정상식이 (ND)군, 고지방식이군으로 나누어 각각의 식이를 4주간 급여한 후 고지방식이군을 설기떡 (SD)군 및 솔잎착즙액설기떡 (PSD)군으로 분류하여 6주간 사육하고, 혈장과 간 및 분변의 지질관련 지표를 분석하였다. PSD군은 설기떡 섭취에 의해 유의하게 높아진 체중증가량을 유의하게 낮추었으며 (p < 0.05), 설기떡 섭취에 의해 유의하게 높아진 혈장 총 콜레스테롤 및 LDL-콜레스테롤을 낮추는 경향을 나타내었다 (p > 0.05). 간 조직과 분변의 지질대사 관련 지표를 분석한 결과 PSD군은 설기떡 섭취에 의해 ND군보다 유의하게 높아진 간 조직의 중성지방 및 총 콜레스테롤과 간 조직의 SREBP2 유전자 발현을 유의하게 낮추는 효과를 나타내었다 (p < 0.05). 또한 PSD군은 SD군보다 분변으로 배출되는 중성지방 함량을 14.8% 높였으며 (p > 0.05), 혈장과 간의 지질과산화를 SD군보다 각각 7.7%, 20.2% 낮추는 효과를 나타내었다 (p > 0.05). 혈장과 적혈구의 항산화활성 관련 지표를 분석한 결과 PSD군은 설기떡 섭취에 의해 낮아진 혈장 ORAC을 높이는 경향을 나타내었으며, 설기떡 섭취에 의해 유의하게 낮아진 간조직의 Mn-SOD 및 CAT 효소활성을 SD군보다 각각 21.5%, 19.6% 유의하게 높이는 효과를 나타내었다. 이상의 결과에서, 고지방식이로 이상지질혈증을 유도한 흰쥐에서 솔잎착즙액설기떡의 섭취는 체중증가억제, 혈장 콜레스테롤 감소, 간 내 콜레스테롤 생합성 억제, 간 조직의 지질수준 감소, 분변으로의 지질배출 증가, 간 조직의 지질과산화 감소, 혈장과 간 조직의 항산화 활성 증가 효능을 나타내는 것으로 확인되었다. 이에 본 연구에서 개발된 솔잎착즙액을 첨가한 설기떡은 지질 감소 효능을 가진 기능성 떡으로써 활용될 수 있을 것으로 기대된다.

Keywords

References

  1. Benjamin EJ, Blaha MJ, Chiuve SE, Cushman M, Das SR, Deo R, et al. American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Heart disease and stroke statistics- 2017 update: a report from the American Heart Association. Circulation 2017; 135(10): e146-e603. https://doi.org/10.1161/CIR.0000000000000485
  2. Ministry of Health and Welfare (KR); Korea Centers for Disease Control and Prevention. Korea health statistics 2015. Korea National Health and Nutrition Examination Survey (KNHANES VI-3). Cheongju: Korea Centers for Disease Control and Prevention; 2016 [cited 2017 Oct 1]. Available from: https://knhanes.cdc.go.kr.
  3. Statistics Korea. Cause of death statistics 2017. Daejeon: Statistics Korea; 2018 [cited 2018 Sep 19]. Available from: https://kostat.go.kr.
  4. Roh E, Ko SH, Kwon HS, Kim NH, Kim JH, Kim CS, et al. Taskforce Team of Diabetes Fact Sheet of the Korean Diabetes Association. Prevalence and management of dyslipidemia in Korea: Korea National Health and Nutrition Examination Survey during 1998 to 2010. Diabetes Metab J 2013; 37(6): 433-449. https://doi.org/10.4093/dmj.2013.37.6.433
  5. Urano S, Hoshi-Hashizume M, Tochigi N, Matsuo M, Shiraki M, Ito H. Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics. Lipids 1991; 26(1): 58-61. https://doi.org/10.1007/BF02544025
  6. Choi HY. Antioxidant activity and quality characteristics of pine needle cookies. J Korean Soc Food Sci Nutr 2009; 38(10): 1414-1421. https://doi.org/10.3746/jkfn.2009.38.10.1414
  7. Lim YS, Bae MJ, Lee SH. Antimicrobial effects of Pinus densiflora Sieb. et Zucc. ethanol extract on Listeria monocytogenes. J Korean Soc Food Sci Nutr 2002; 31(2): 333-337. https://doi.org/10.3746/jkfn.2002.31.2.333
  8. Lee E. Effect of powdered pine needle (Pinus densiflora seib et Zucc.) on serum and liver lipid composition and antioxidative capacity in rats fed high oxidized fat. J Korean Soc Food Sci Nutr 2003; 32(6): 926-930. https://doi.org/10.3746/jkfn.2003.32.6.926
  9. Kim JS. Evaluation of in vitro antioxidant activity of the water extract obtained from dried pine needle (Pinus densiflora). Prev Nutr Food Sci 2018; 23(2): 134-143. https://doi.org/10.3746/pnf.2018.23.2.134
  10. Choi HD, Koh YJ, Choi IW, Kim YS, Park YK. Anticariogenic activity and glucosyltransferase inhibitory effects of extracts from pine needle and twig. Korean J Food Sci Technol 2007; 39(3): 336-341.
  11. Yen GC, Duh PD, Huang DW, Hsu CL, Fu TY. Protective effect of pine (Pinus morrisonicola Hay.) needle on LDL oxidation and its anti-inflammatory action by modulation of iNOS and COX-2 expression in LPS-stimulated RAW 264.7 macrophages. Food Chem Toxicol 2008; 46(1): 175-185. https://doi.org/10.1016/j.fct.2007.07.012
  12. Kim SH, Hwang SY, Park OS, Kim MK, Chung YJ. Effect of Pinus densiflora extract on blood glucose level, OGTT and biochemical parameters in streptozotocin induced diabetic rats. J Korean Soc Food Sci Nutr 2005; 34(7): 973-979. https://doi.org/10.3746/jkfn.2005.34.7.973
  13. Jeon JR, Kim JY, Lee KM, Cho DH. Anti-obese effects of mixture contained pine needle, black tea and green tea extracts. J Korean Soc Appl Biol Chem 2005; 48(4): 375-381.
  14. Ren P, Ren X, Cheng L, Xu L. Frankincense, pine needle and geranium essential oils suppress tumor progression through the regulation of the AMPK/mTOR pathway in breast cancer. Oncol Rep 2018; 39(1): 129-137. https://doi.org/10.3892/or.2017.6067
  15. Park MK, Lee JM, Park CH, In MJ. Quality characteristics of Sulgidduk containing chlorella powder. J Korean Soc Food Sci Nutr 2002; 31(2): 225-229. https://doi.org/10.3746/jkfn.2002.31.2.225
  16. Lee JH, Yoon SJ. Quality characteristics of Sulgidduk prepared with different amounts of green laver powder. Korean J Food Cookery Sci 2008; 24(1): 39-45.
  17. Cho JS, Choi MY, Chang YH. Quality characteristics of Sulgiduk added with Lentinus edodes sing powder. J East Asian Soc Diet Life 2002; 12(1): 55-64.
  18. Yoon SJ. Quality characteristics of Sulgitteok with lotus leaf powder. Korean J Food Cookery Sci 2007; 23(4): 433-442.
  19. Park GY, Li HX, Hwang ID, Cheong HS. The functional effects of fermented pine needle extract. Korean J Biotechnol Bioeng 2006; 21(5): 376-383.
  20. Puntel RL, Roos DH, Grotto D, Garcia SC, Nogueira CW, Rocha JB. Antioxidant properties of Krebs cycle intermediates against malonate pro-oxidant activity in vitro: a comparative study using the colorimetric method and HPLC analysis to determine malondialdehyde in rat brain homogenates. Life Sci 2007; 81(1): 51-62. https://doi.org/10.1016/j.lfs.2007.04.023
  21. Kim GN, Shin JG, Jang HD. Antioxidant and antidiabetic activity of Dangyuja (Citrus grandis Osbeck) extract treated with Aspergillus saitoi. Food Chem 2009; 117(1): 35-41. https://doi.org/10.1016/j.foodchem.2009.03.072
  22. Bogdanska JJ, Korneti P, Todorova B. Erythrocyte superoxide dismutase, glutathione peroxidase and catalase activities in healthy male subjects in Republic of Macedonia. Bratisl Lek Listy 2003; 104(3): 108-114.
  23. Carrillo MC, Kanai S, Nokubo M, Kitani K. (-) deprenyl induces activities of both superoxide dismutase and catalase but not of glutathione peroxidase in the striatum of young male rats. Life Sci 1991; 48(6): 517-521. https://doi.org/10.1016/0024-3205(91)90466-O
  24. Ahn Y, Gebereamanuel MR, Oh EK, Kwon O. Inhibitory effects of persimmon (Diospyros kaki Thumb.) against diet-induced hypertriglyceridemia/hypercholesterolemia in rats. J Nutr Health 2017; 50(3): 225-235. https://doi.org/10.4163/jnh.2017.50.3.225
  25. Grundy SM. Hypertriglyceridemia, atherogenic dyslipidemia, and the metabolic syndrome. Am J Cardiol 1998; 81(4A): 18B-25B. https://doi.org/10.1016/S0002-9149(98)00033-2
  26. Matsubara M, Maruoka S, Katayose S. Decreased plasma adiponectin concentrations in women with dyslipidemia. J Clin Endocrinol Metab 2002; 87(6): 2764-2769. https://doi.org/10.1210/jcem.87.6.8550
  27. Hwang KH, Jung LH, Cho NC, Yoo YK, Park PS, Noh YH, et al. The effect of concentrated onion juice in a body composition, serum electrolytes and lipids levels on hyperlipidemia. Korean J Food Nutr 2003; 16(1): 35-45.
  28. Lee JJ, Park MR, Kim AR, Lee MY. Effects of ramie leaves on improvement of lipid metabolism and antiobesity effect in rats fed a high fat/high cholesterol diet. Korean J Food Sci Technol 2011; 43(1): 83-90. https://doi.org/10.9721/KJFST.2011.43.1.083
  29. Jeon JR, Kim JY. Effects of pine needle extract on differentiation of 3T3-L1 preadipocytes and obesity in high-fat diet fed rats. Biol Pharm Bull 2006; 29(10): 2111-2115. https://doi.org/10.1248/bpb.29.2111
  30. Choi JH, Kim DI, Choi MG, Cho WK, Kim CK. Effects of pine needle butanol fraction on membrane fluidity and oxidative stress in liver membranes of rats. J Korean Soc Food Sci Nutr 2003; 32(7): 1082-1087. https://doi.org/10.3746/jkfn.2003.32.7.1082
  31. Kang YH, Park YK, Ha TY, Moon KD. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr 1996; 25(3): 367-373.
  32. Lee E, Choi MY. Effect of pine needle on lipid composition and TBARS in rat fed high cholesterol. Korean J Food Sci Technol 2000; 32(5): 1186-1190.
  33. Jang JY, Kim MJ, Lee MK, Kim DJ. Effect of pine needle water extract on cadmium-induced oxidative stress in rats. J Korean Soc Food Sci Nutr 2007; 36(4): 411-418. https://doi.org/10.3746/jkfn.2007.36.4.411
  34. Kang JH, Kang JH. Effect of the consumption values of rice cake type rice processed food on attitude and purchase intention. Int J Tourism Hosp Res 2017; 31(9): 217-232. https://doi.org/10.21298/IJTHR.2017.09.31.9.217
  35. Yoon SJ, Oh IS. Usage status of traditional rice cake as a meal substitute and analysis on the selection attributes affecting purchase. Korean J Culinary Res 2014; 20(2): 38-53.
  36. Kim CH, Lee JH. The study on the consumer' perception and purchasing behavior of rice cake as a meal. Korean J Culinary Res 2007; 13(2): 59-68.
  37. Sin MK, Han GJ. Effects of water extracts green tea scented with lotus Nelumbo nucifera gaertner flower on the serum lipid concentrations in rats fed high fat. J East Asian Soc Diet Life 2005; 15(1): 57-64.
  38. Harris WS, Connor WE, McMurry MP. The comparative reductions of the plasma lipids and lipoproteins by dietary polyunsaturated fats: salmon oil versus vegetable oils. Metabolism 1983; 32(2): 179-184. https://doi.org/10.1016/0026-0495(83)90226-3
  39. Sanders TA, Hochland MC. A comparison of the influence on plasma lipids and platelet function of supplements of omega 3 and omega 6 polyunsaturated fatty acids. Br J Nutr 1983; 50(3): 521-529. https://doi.org/10.1079/BJN19830123
  40. Droubay PE, Puppione DL. Dietary fat-induced postprandial lipemia: effect on arterial oxygen saturation and plasma lactate, triglyceride, and cholesterol levels in subjects with angina pectoris. Am J Clin Nutr 1980; 33(6): 1199-1207. https://doi.org/10.1093/ajcn/33.6.1199
  41. Cho SY, Kim MJ. The effect of plantaginis semen on serum and hepatic lipid metabolism in fed high and low fat diets. J Korean Soc Food Nutr 1995; 24(4): 517-522.
  42. Sun JH, Liu X, Cong LX, Li H, Zhang CY, Chen JG, et al. Metabolomics study of the therapeutic mechanism of Schisandra Chinensis lignans in diet-induced hyperlipidemia mice. Lipids Health Dis 2017; 16(1): 145. https://doi.org/10.1186/s12944-017-0533-3
  43. Vijayakumar R, Nachiappan V. Cassia auriculata flower extract attenuates hyperlipidemia in male Wistar rats by regulating the hepatic cholesterol metabolism. Biomed Pharmacother 2017; 95: 394-401. https://doi.org/10.1016/j.biopha.2017.08.075
  44. Kritchevsky D, Tepper SA. Experimental atherosclerosis in rabbits fed cholesterol-free diets: influence of chow components. J Atheroscler Res 1968; 8(2): 357-369. https://doi.org/10.1016/S0368-1319(68)80070-5
  45. Chung SY, Kim SA, Kim SH, Kim HS, Kim GJ, Kim HS, et al. Effects of Ganoderma lucidum on lipid metabolism in dietary hypercholesterolemic rats. J Korean Soc Food Nutr 1990; 19(2): 180-186.
  46. Huang J, Wang Y, Ying C, Liu L, Lou Z. Effects of mulberry leaf on experimental hyperlipidemia rats induced by high-fat diet. Exp Ther Med 2018; 16(2): 547-556.
  47. Wang H, Chen G, Ren D, Yang ST. Hypolipidemic activity of okra is mediated through inhibition of lipogenesis and upregulation of cholesterol degradation. Phytother Res 2014; 28(2): 268-273. https://doi.org/10.1002/ptr.4998
  48. Kang YH, Park YK, Oh SR, Moon KD. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 1995; 27(6): 978-984.

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