Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Enhancement of Laccase Production from Wood-Rotting Fungus by Co-Culture with Trichoderma longibrachiatum

  • Jung, Hyun-Chae (Department of Forest Resources, Sunchon National University)
  • Received : 2018.12.20
  • Accepted : 2019.03.06
  • Published : 2019.03.25
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Abstract

This work aimed to evaluate the influence of culture conditions on laccase production in the co-culture of wood-rotting fungus with Trichoderma sp. The effects of infection extent, infection time, and culture filtrate of Trichoderma sp. on the laccase production by wood-rotting fungus in co-culture were examined. T. rubrum LKY-7 and T. longibrachiatum were selected as fungi which are effective in co-culture for laccase production. A significant increase in laccase activity was observed when T. rubrum LKY-7 was co-cultured with T. longibrachiatum in glucose-peptone liquid medium, yielding an increase of more than 5 times in laccase activity, as compared with control. Laccase production by T. rubrum LKY-7 during co-culturing was significantly influenced by the infection extent and the infection time of T. longibrachiatum. Maximal laccase activity was obtained when T. rubrum LKY-7 culture was infected by T. longibrachiatum after 3 days of cultivation at an inoculum size ratio of 0.5 to 1. The addition of culture filtrate or autoclaved mycelium of T. longibrachiatum to T. rubrum LKY-7 culture did not significantly enhance laccase production by T. rubrum LKY-7 as compared with control (mono cultures of T. rubrum LKY-7).

Keywords

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Fig. 1. Effect of the infection extent of T. longibrachiatum on laccase production by T. rubrum LKY-7.

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Fig. 2. Effect of the infection time of T. longibrachiatum on laccase production by T. rubrum LKY-7. A, Inoculum size ratio of 0.5 to 1; B, Inoculum size ratio of 1 to 1.

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Fig. 3. Effects of the culture filtrate and the autoclaved mycelium of T. longibrachiatum on laccase pro-duction by T. rubrum LKY-7.

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Fig. 4. Detection of laccase isozymes by native PAGE. Lane 1, self-paired culture of LKY-7 in GPA Lane 2, co-culture in GPA Lane 3, mono-culture of LKY-7 in GPB Lane 4, co-culture in GPB.

Table 1. Laccase activities of wood-rotting fungi during co-culturing with Trichoderma strains in GPA

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