The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Three Unreported Endophytic Fungi Isolated from Conifer Leaves of Pinus densiflora in Korea

소나무 침엽에서 분리된 3종의 국내 미기록 내생균

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2019.02.26
  • Accepted : 2019.03.15
  • Published : 2019.03.01
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Abstract

We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

본 연구에서는 전북 부안군에 서식하는 소나무의 침엽에서 내생균을 분리하였다. 분리된 균주는 internal transcribed spacer 영역, rDNA의 large subunit 영역, beta-tubulin 영역의 DNA 염기서열을 분석하여 동정하였다. 연구 과정에서 3종의 국내 미기록 내생균 균주를 확인하였으며, 확인된 종은 Paracamarosporiumhawaiiense, Tubakia dryina, Zasmidiumfructigenum이다. 확인된 미기록 균주의 형태적 특성 및 계통적 분석의 결과에 대해 서술하였다.

Keywords

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Fig. 1. Colonies of Paracamarosporium hawaiiense 16B647 grown for 7 days on potato dextrose agar (A) and malt extract agar (E), conidia (D, H). Colonies of Tubakia dryina (16B554) grown for 7 days on PDA (B) and MEA (F), conidiophore and conidia (I, J). Colonies of Zasmidium fructigenum (16B210) grown for 7 days on PDA (C) and MEA (G), conidiophore and conidia (K, L). (scale bars =10 μm).

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Fig. 2. Neighbor-joining phylogenetic tree based on a concatenated alignment of internal transcribed spacer and large subunit sequences. Phaeosphaeria pontiformis was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.

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Fig. 3. Neighbor-joining phylogenetic tree based on a concatenated alignment of internal transcribed spacer, large subunit and beta-tubulin sequences. Xylaria hypoxylon was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.

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Fig. 4. Neighbor-joining phylogenetic tree based on a concatenated alignment of internal transcribed spacer and large subunit sequences. Xylaria hypoxylon was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.

Table 1. Morphological characteristics of fungal strains isolated from Pinus densifora.

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Table 2. List of fungal species used in phylogenetic analysis with the GenBank accession numbers

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