Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Functional and Emulsifying Properties of Balloonflower Seeds Oil Extracted by Supercritical Carbon Dioxide

초임계 이산화탄소 추출로 얻은 도라지 종자유의 기능성 및 에멀젼 특성

  • Kim, Yangji (Department of Food and Nutrition, Dongduk Women's University) ;
  • Imm, Jee-Young (Department of Food and Nutrition, Kookmin University) ;
  • Kim, In-Ho (Research Division of Industrial Support, Korea Food Research Institute) ;
  • Kim, Seok Joong (Department of Food and Nutrition, Dongduk Women's University)
  • 김양지 (동덕여자대학교 식품영양학과) ;
  • 임지영 (국민대학교 식품영양학과) ;
  • 김인호 (한국식품연구원 산업지원연구본부) ;
  • 김석중 (동덕여자대학교 식품영양학과)
  • Received : 2019.03.06
  • Accepted : 2019.03.28
  • Published : 2019.03.31
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Abstract

In this study, functional and emulsifying properties of oil extracted by supercritical carbon dioxide from balloonflower(Platycodon grandiflorum A. DC) seeds were investigated. The oil was lower in total polyphenol content(8 mg/100 g), but higher in ${\alpha}$-tocopherol(14.15 mg/100 g), ${\beta}$-sitosterol(116 mg/100 g) and stigmasterol(8 mg/100 g) contents than seeds. Based on DPPH radical scavenging activity, the oil($IC_{50}=1235.5{\mu}g/mL$) showed similar antioxidant activity to the seeds($IC_{50}=1138.2{\mu}g/mL$). At 1%(w/w) lecithin, O/W emulsion with balloonflower seeds oil had turbidity, microscopic image, mean particle size and emulsion stability similar to emulsion with soybean oil, but had lower turbidity and emulsion stability and larger mean particle size than emulsion with perilla seeds oil. Its surface tension was similar to perilla oil emulsion.

본 연구에서는 초임계 이산화탄소를 사용해 도라지(balloonflower, Platycodon grandiflorum A. DC) 종자로부터 추출한 유지의 기능성과 에멀전 특성에 대해 조사하였다. 종자에 비해 도라지 종자유는 총 polyphenol(8 mg/100 g)의 함량이 낮았으나 ${\alpha}$-tocopherol(14.15 mg/ 100 g), ${\beta}$-sitosterol(116 mg/100 g) 및 stigmasterol(8 mg/100 g)의 함량은 높았다. DPPH 라디칼 소거능으로 분석한 종자유($IC_{50}=1235.5{\mu}g/mL$)의 항산화능은 종자($IC_{50}=1138.2{\mu}g/mL$)와 유사하였다. 1%(w/w) lecithin 농도에서 도라지 종자유를 사용해 제조한 에멀젼은 탁도, 지방구 입자 크기 및 형상, 유화안정성에서 콩기름을 사용한 에멀젼과 유사하였으나 들기름 사용 에멀젼보다는 탁도 및 유화안정성이 낮았고 지방구 입자는 크게 나타났다. 도라지 종자유 에멀젼의 표면장력은 들기름 에멀젼과 유사하였다.

Keywords

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Fig. 1. Yield of balloonflower seeds oils prepared by supercritical CO2, hexane, and press extractions.

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Fig. 2. DPPH radical scavenging activity ofballoonflower seeds and seeds oil.

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Fig. 3. Turbidity changes of emulsions with 1% lecithin depending on oil content.

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Fig. 4. Optical microscopic images(×400) of O/W emulsions with 1% lecithin at different oil contents. Scale bar on right bottom of image indicates 100 μm size.

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Fig. 5. Particle size analysis of emulsion containing 30% oil and 1% lecithin.

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Fig. 6. Separation of cream layer from emulsion containing 30% oil and 1% lecithin depending on centrifugal force.

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Fig. 7. Surface tension of emulsions containing 30% oil and 1% lecithin.

Table 1. HPLC analysis condition of vitamins

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Table 2. Content of functional ingredients of balloonflower seeds and seeds oil

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