Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Growth of Preantral Follicle and Maturation of Intrafollicular Oocyte from Aged Mice

  • Yoon, Jung-Ah (Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University) ;
  • Choi, Jung-Kyu (Department of Physiology, School of Medicine, CHA University)
  • Received : 2018.12.18
  • Accepted : 2019.03.15
  • Published : 2019.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to recover the ovarian function through in vitro culture of preantral follicles from aged mice. First, we isolated the preantral follicles from ovaries of sixty-seven-week old B6D2F1 mice with decreased fecundity to know how many follicles were present in them, which was 6 preantral follicles including 2 primary, 2 early secondary and late secondary follicles from 8 aged mice. It was confirmed that a few follicles (~2) were present in aged mice through histological analysis compared to adult mice as control. The 9 days of in vitro culture of preantal follicles showed in vitro growth and induced maturation after treatment with hCG (2.5 IU/mL) and EGF (5 ng/mL). Cumulus cells in the cumulus-oocyte complexes (COCs) were removed using hyaluronidase and oocytes at the germinal vesicle (GV) and GV breakdown (GVBD) were obtained from preantral follicle culture of aged mice in vitro. In conclusion, these observations demonstrated that there still were a few preantral follicles in the ovaries of 67 week-old mice, which we were able to culture in vitro and oocytes were obtained from them. This study proposed an in vitro culture system using preantral follicle as a therapeutic strategy for fertility preservation in humans for assisted reproductive medicine.

Keywords

References

  1. Barnett KR, Schilling C, Greenfeld CR, Tomic D and Flaws JA. 2006. Ovarian follicle development and transgenic mouse models. Hum. Reprod. Update. 12:537-55. https://doi.org/10.1093/humupd/dml022
  2. Broekmans FJ, Soules MR and Fauser BC. 2009. Ovarian aging: mechanisms and clinical consequences. Endocr. Rev. 30:465-93. https://doi.org/10.1210/er.2009-0006
  3. Choi JK, Agarwal P and He X. 2013. In vitro culture of early secondary preantral follicles in hanging drop of ovarian cell-conditioned medium to obtain MII oocytes from outbred deer mice. Tissue Eng Part A. 19(23-24):2626-37. https://doi.org/10.1089/ten.tea.2013.0055
  4. Choi JK, Agarwal P, Huang H, Zhao S and He X. 2014. The crucial role of mechanical heterogeneity in regulating follicle development and ovulation with engineered ovarian microtissue. Biomaterials. 35(19): 5122-28. https://doi.org/10.1016/j.biomaterials.2014.03.028
  5. Choi JK, Lee JH, Lee ST, Choi MH, Gong SP, Lee EJ and Lim JM. 2007. Developmental competence of intrafollicular oocytes derived from preantral follicle culture with different protocols after parthenogenetic activation. Asian-Aust. J. Anim. Sci. 20(8):1190-95. https://doi.org/10.5713/ajas.2007.1190
  6. Donnez J and Dolmans MM. 2010. Cryopreservation and transplantation of ovarian tissue. Clin. Obstet. Gynecol. 53:787-96. https://doi.org/10.1097/GRF.0b013e3181f97a55
  7. Filatov MA, Khramova YV and Semenova ML. 2015. In vitro mouse ovarian follicle growth and maturation in alginate hydrogel: current state of the art. Acta Naturae. 7: 48-56.
  8. Hassold T and Hunt P. 2001. To err (meiotically) is human: the genesis of human aneuploidy. Nat. Rev. Genet. 2:280-91. https://doi.org/10.1038/35066065
  9. Higuchi CM, Maeda Y, Horiuchi T, Yamazaki Y. 2015. A simplified method for three-dimensional (3D) ovarian tissue culture yielding oocytes competent to produce full-term offspring in mice. Plos one. 16;10(11):e0143114. https://doi.org/10.1371/journal.pone.0143114
  10. Jeruss JS and Woodruff TK. 2009. Preservation of fertility in patients with cancer. N. Engl. J Med. 360:902-11. https://doi.org/10.1056/NEJMra0801454
  11. Laven JS. 2016. Primary ovarian insufficiency. Semin. Reprod. Med. 34(4):230-4. https://doi.org/10.1055/s-0036-1585402
  12. Wang Y, Anazodo A and Logan S. 2018. Systematic review of fertility preservation patient decision aids for cancer patients. Psychooncology.
  13. Woodruff TK. 2008. Making eggs: is it now or later? Nat. Med. 14:1190-91. https://doi.org/10.1038/nm1108-1190
  14. Woodruff TK and Shea LD. 2011. A new hypothesis regarding ovarian follicle development: ovarian rigidity as a regulator of selection and health. Journal of Assisted Reproduction and Genetics. 28(1):3-6. https://doi.org/10.1007/s10815-010-9478-4
  15. Wu J and Tian Q. 2007. Role of follicle stimulating hormone and epidermal growth factor in the development of porcine preantral follicle in vitro. Zygote. 15(3):233-40. https://doi.org/10.1017/S0967199407004194
  16. Wu J, Xu B and Wang W. 2007. Effects of luteinizing hormone and follicle stimulating hormone on the developmental competence of porcine preantral follicle oocytes grown in vitro. J Assist. Reprod. Genet. 24(9):419-24. https://doi.org/10.1007/s10815-007-9154-5