Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Molecular and Morphological Evidence of Hepatotoxicity after Silver Nanoparticle Exposure: A Systematic Review, In Silico, and Ultrastructure Investigation

  • Sooklert, Kanidta (Nanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University) ;
  • Wongjarupong, Asarn (Department of Orthopedics, Queen SavangVadhana Memorial Hospital) ;
  • Cherdchom, Sarocha (Nanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University) ;
  • Wongjarupong, Nicha (Department of Physiology, Faculty of Medicine, Chulalongkorn University) ;
  • Jindatip, Depicha (Department of Anatomy, Faculty of Medicine, Chulalongkorn University) ;
  • Phungnoi, Yupa (Department of Biology, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University) ;
  • Rojanathanes, Rojrit (Department of Chemistry, Faculty of Science, Chulalongkorn University) ;
  • Sereemaspun, Amornpun (Nanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University)
  • Received : 2018.10.29
  • Accepted : 2019.01.29
  • Published : 2019.07.15
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Abstract

Silver nanoparticles (AgNPs) have been widely used in a variety of applications in innovative development; consequently, people are more exposed to this particle. Growing concern about toxicity from AgNP exposure has attracted greater attention, while questions about nanosilver-responsive genes and consequences for human health remain unanswered. By considering early detection and prevention of nanotoxicology at the genetic level, this study aimed to identify 1) changes in gene expression levels that could be potential indicators for AgNP toxicity and 2) morphological phenotypes correlating to toxicity of HepG2 cells. To detect possible nanosilver-responsive genes in xenogenic targeted organs, a comprehensive systematic literature review of changes in gene expression in HepG2 cells after AgNP exposure and in silico method, connection up- and down-regulation expression analysis of microarrays (CU-DREAM), were performed. In addition, cells were extracted and processed for transmission electron microscopy to examine ultrastructural alterations. From the Gene Expression Omnibus (GEO) Series database, we selected genes that were up- and down-regulated in AgNPs, but not up- and down-regulated in silver ion exposed cells, as nanosilver-responsive genes. HepG2 cells in the AgNP-treated group showed distinct ultrastructural alterations. Our results suggested potential representative gene data after AgNPs exposure provide insight into assessment and prediction of toxicity from nanosilver exposure.

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References

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