Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of anti-inflammatory on Perilla frutescens var. crispa Induced by mutants with γ-Ray

감마선을 이용한 육종 차조기의 항염증 효과

  • 심부용 (중부대학교 산학협력단) ;
  • 박정현 ((주)에스에프씨바이오) ;
  • 김성규 ((주)에스에프씨바이오) ;
  • 지중구 (중부대학교 한방건강관리학과)
  • Received : 2019.06.07
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

The purpose of this study was to confirmed anti-inflammatory effect the apple Induced by mutants with ${\gamma}-Ray$ extract. Cell viability was assessed by MTT assay using RAW 264.7 cells. The extracts measured through changes in the levels of reactive oxygen species (ROS), nitric oxide (NO), inflammatory cytokines, NF-kB, and COX-2 on LPS-induced RAW 264.7 cells. All test results were analyzed by ELISA reader, Luminex and RT-PCR. In result, the extracts was not toxic below in 25 ug/ml, and extracts was inhibited the productions nitric oxide, ROS, cytokines (IL-1b, IL-6, TNF-a), NF-kB and COX-2 in LPS-induced RAW 264.7 cells. Also, the expression levels were decreased on mRNA of $NF-{\kappa}B$ and COX-2. In other words, Perilla frutescens var. crispa Induced by mutants with ${\gamma}-Ray$ extracts showed significant anti-inflammatory effect. These results may be developed as a raw material for new health food and therapeutics to ease the related to the above mediators.

본 연구는 감마선을 이용한 육종 차조기 추출물을 통해 항염증 효능을 평가하고자 하였다. RAW 264.7 세포에서 MTT를 통해 세포 생존율을 평가하였으며, LPS로 유도한 RAW 264.7 세포에서 ROS, NO, 염증성 사이토카인, $NF-{\kappa}B$, COX-2 등을 ELISA, Luminex 및 PCR로 측정하였다. 그 결과, 육종 차조기 추출물은 $25{\mu}g/m{\ell}$ 이하에서 세포독성이 없었으며, LPS로 유도된 RAW 264.7 세포에서 ROS, NO, 사이토카인($IL-1{\beta}$, IL-6, $TNF-{\alpha}$)의 생성을 억제하였다. 또한, $NF-{\kappa}B$, COX-2의 발현을 감소시켜 육종 차조기 추출물은 뛰어난 항염증 효과를 보였다. 이와 같은 결과는 염증 매개체로 인해 발생하는 질환을 개선하기 위한 새로운 건강식품 및 치료제의 원료로 개발될 수 있다.

Keywords

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Fig. 1. Effect of Perilla frutescens var. crispa Induced by mutants with γ-Ray extracts on cell viability in RAW 264.7 cells.

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Fig. 2. Effect of Perilla frutescens var. crispa Induced by mutants with γ-Ray extracts on ROS levels in RAW 264.7 cells.

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Fig. 3. Effect of Perilla frutescens var. crispa Induced by mutants with γ-Ray extracts on NO levels in RAW 264.7 cells.

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Fig. 4. Effect of Perilla frutescens var. crispa Induced by mutants with γ-Ray extracts on cytokine IL-1β(A), IL-6(B), and TNF-α(C) levels in RAW 264.7 cells.

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Fig. 5. Effect of Perilla frutescens var. crispa Induced by mutants with γ-Ray extracts on mRNA NF-κB (A), and COX-2 (B) expression in RAW 264.7 cells.

Table 1. The Sequences of Primers in This Study

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