Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Effect of Photoprotective activities of Poncirustrifoliata immature Fruit extract and Naringin compound

지실 추출물과 Naringin의 광방어 효과

  • Park, Sang-Hee (Division of Skin health, Daejeon institute Science and Technology) ;
  • Kwak, In-Sil (Dept. of Beauty Art, Howon University)
  • 박상희 (대전과학기술대학교 피부보건과) ;
  • 곽인실 (호원대학교 미용예술학과)
  • Received : 2019.04.23
  • Accepted : 2019.07.20
  • Published : 2019.07.28
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Abstract

The study studied dermal protection and crease improvement from the sunlight of the lipid extract and narinjin. Sunlight was investigated in HR-1 (motherless mice) to identify changes in epithelial thickness and changes in collagen fibers, which account for around 90% of dermis, as the inhibitory efficacy of collagenase dissolving collagen also plays an important role in wrinkles. The experiment was validated using narinjin and jisil extract. First: The components of jisil and narinjin were analyzed. Second, antioxidant capabilities were confirmed with DPPH. Third: The inhibitory activity of collagen was measured. Studies have shown that the skin's upper skin thickness suppression of dermal extracts and narinzine has increased and that collagen thicknesses and wrinkles have decreased significantly compared to controls.

본 연구는 지실추출물과 나린진의 햇빛으로부터 피부 보호 및 주름개선에 대한 연구를 하였다. HR-1(무모쥐)에 햇빛을 조사하여 상피 두께의 변화와 진피의 90% 정도를 차지하는 콜라겐 섬유의 변화를 확인하였다, 콜라게나아제의 억제 효능도 주름에 중요하게 작용하므로 같이 확인하였다. 실험은 나린진과 지실추출물을 사용하여 효능을 검증하였다. 첫째: 지실과 나린진의 성분을 분석하였다. 둘째: DPPH로 항산화 능력을 확인하였다. 셋째: 콜라겐의 변화를 측정하였다. 연구 결과로 지실추출물 및 나린진이 피부 상피두께 억제와 피부의 콜라겐 두께가 상승하였고 콜라겐 분해 효소 억제 및 주름이 대조군에 비하여 감소되었으며 유의한 차이가 나타났다.

Keywords

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Fig. 1. Inhibition of skin thickening by PT_iF and naringin on epidermis induced by UVB irradiation.

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Fig. 2. Inhibition of skin thickening by vehicle control on epidermis induced by UVB irradiation.

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Fig. 3. HR-1 hairless male mice dorsal skin

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Fig. 4. Inhibition of skin thickening by 2.0% PT_iF on epidermis induced by UVB irradiation.

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Fig. 5. Inhibition of skin thickening by 0.25% naringin on epidermis induced by UVB irradiation.

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Fig. 6. Inhibition of skin thickening by 1.0% naringin on epidermis induced by UVB irradiation.

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Fig. 7. HR-1 hairless male mice dorsal skin

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Fig. 8. Collagen deposition by vehicle control on epidermis induced by UVB irradiation.

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Fig. 9. Collagen deposition by 0.5% PT_iF extract cream-ointment on epidermis induced by UVB irradiation.

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Fig. 10. Collagen deposition by 2% PT_iF extract cream-ointment on epidermis induced by UVB irradiation.

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Fig. 11. Collagen deposition by 0.25% naringin cream-ointment on epidermis induced by UVB irradiation.

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Fig. 12. Collagen deposition by 1.0% naringin cream-ointment on epidermis induced by UVB irradiation.

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Fig. 13. HR-1 hairless male mice dorsal skin on wrinkle formation

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Fig. 14. Effects of vihicle control on UVB-induced wrinkle formation in hairless mice

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Fig. 15. Effects of 2% PT_iF extract cream-ointment on UVB-induced wrinkle formation in hairless mice

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Fig. 16. Effects of 0.5% PT_iF extract cream-ointment on UVB-induced wrinkle formation in hairless mice

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Fig. 17. Effects of 1.0% naringin cream-ointment on UVB-induced wrinkle formation in hairless mice

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Fig. 18. Effects of 0.25% naringin cream-ointment on UVB-induced wrinkle formation in hairless mice

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Fig. 19. HR-1 hairless male mice dorsal skin on wrinkle photograph.

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Fig. 20. Effects of vihicle control on UVB-induced wrinkle photograph in hairless mice

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Fig. 21. Effect of 2% PT_iF Extracted Cream Ointment on UVB-induced Wrinkle Photographs in Unhulled Mice

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Fig. 22. Effect of 0.5% PT_iF Extract Cream Ointment on UVB-Induced Wrinkle Photographs of Rehmannia Rat

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Fig. 23. Effect of 1.0% Naringin cream ointment on UVB-induced wrinkles in hairless mice

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Fig. 24. Effect of 0.25% Naringin Cream Ointment on UVB-Induced Wrinkle Photographs of Hairless Rats

Table 1. UVB irradiation and medication schedule

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Table 2. Poncirus trifoliata Naringin in immature fruit and ointment cream formulations.

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Table 3. Primer sequences (wrinkle-related genes) for real-time PCR analysis.

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Table 4. Primer sequences (wrinkle-related genes) for real-time PCR analysis.

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Table 5. Taqman probes and primer sequences (pro-inflammatory gene) for real-time PCR analysis.

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