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Form and Embryonic Characteristics of Pedicularis hallaisanensis Seeds As Endangered Wild Species II-Class Using Host Plants

숙주식물을 활용한 멸종위기야생식물II급 한라송이풀 종자의 형태 및 발아특성

  • Kim, Lim-Kyu (Gayasan National Park Office, Korea National Park Service) ;
  • Park, Eun-Hee (Gayasan National Park Office, Korea National Park Service) ;
  • Gang, GeunHye (Species Restoration Technology Institute, Korea National Park Service) ;
  • Hwang, Boo-Yeong (Species Restoration Technology Institute, Korea National Park Service) ;
  • Jung, Hyun-Jin (Species Restoration Technology Institute, Korea National Park Service) ;
  • Kim, Min-Yong (Department of Forest Environmental Resources(Institute of Agriculture Llife Science), Gyeongsang National University) ;
  • Park, Jeong-geun (Geoje City Hall) ;
  • Park, Sam-Bong (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Kim, Bong-Gyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Choo, Gab-Chul (Department of Forest Resources, Gyeongnam National University of Science and Technology)
  • 김임규 (국립공원공단가야산국립공원사무소) ;
  • 박은희 (국립공원공단가야산국립공원사무소) ;
  • 강근혜 (국립공원공단종복원기술원) ;
  • 황부영 (국립공원공단종복원기술원) ;
  • 정현진 (국립공원공단종복원기술원) ;
  • 김민영 (경상대학교 산림자원학과(농업생명과학연구원)) ;
  • 박정근 (거제시청) ;
  • 박삼봉 (경남과학기술대학교 산림자원학과) ;
  • 김봉규 (경남과학기술대학교 산림자원학과) ;
  • 추갑철 (경남과학기술대학교 산림자원학과)
  • Received : 2019.06.17
  • Accepted : 2019.07.23
  • Published : 2019.09.30

Abstract

This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was $0.47{\pm}0.07mm$, width $0.16{\pm}0.006mm$, and thickness $0.12{\pm}0.01mm$. The weight of one seed was $0.0003{\pm}0.0001mg$, and 1000 seeds weighed $4.59{\pm}0.02mg$. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at $4^{\circ}C$. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at $4^{\circ}C$ were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination. The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.

본 연구는 멸종위기야생식물 II급인 한라송이풀(Pedicularis hallaisanensis)을 대상으로 종자를 통한 번식체계 확립 가능성을 조사하기 위해 수행하였다. 한라송이풀의 종자 형태는 난형이고, 종피는 진갈색을 띠고 있다. 종자의 단면을 잘라 확인한 배는 왜소형(Dwarf type)이었다. 종자의 길이는 평균(${\pm}$표준편차) $0.47{\pm}0.07mm$이고, 너비는 $0.16{\pm}0.006mm$, 두께는 $0.12{\pm}0.01mm$로 나타났다. 종자 1립의 무게는 $0.0003{\pm}0.0001mg$이며, 천립중은 $4.59{\pm}0.02mg$으로 나타났다. Tetrazolium (TZ) 검정에 의한 한라송이풀 종자의 활력도는 75.33%로 나타났다. 한라송이풀을 4주간 $4^{\circ}C$에 저장한 후의 발아율은 71%로 가장 높았고, 6주간 처리 64%, 8주간 처리 60%로 나타나 저장 기간이 증가할수록 발아율은 낮아지는 경향을 보였다. 한라송이풀 종자에 영향을 미치는 숙주식물의 영향은 쑥에서 파종 후 53.5일에, 구절초는 62.5일에 최초로 발아를 하였다. 두 숙주식물에서 164일 이후에는 발아한 종자를 확인하지 못하였다. 쑥을 사용한 경우, 한라송이풀의 발아율은 평균 45.5%, 구절초는 19.5%로 나타났다. 평균 발아일수는 쑥에서 70.2일, 구절초에서는 46.8일로 나타났다.

Keywords

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