Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Method Development for Determination of Chlorogenic Acid and Arbutin Contents in Fruits by UHPLC-MS/MS

UHPLC-MS/MS를 이용한 과일류 중 클로로젠산 및 알부틴 동시분석법 개발

  • Choi, Young-Ju (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Jeon, Jong-Sup (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Kim, Woon-Ho (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Jung, You-Jung (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Ryu, Ji-Eun (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Choi, Jong-Chul (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Chae, Kyung-Suk (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Lee, Jin-Hee (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Do, Young-Sook (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Park, Young-Bae (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Yoon, Mi-Hye (Anyang Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment)
  • 최영주 (경기도보건환경연구원 안양농수산물검사소) ;
  • 전종섭 (경기도보건환경연구원 안양농수산물검사소) ;
  • 김운호 (경기도보건환경연구원 안양농수산물검사소) ;
  • 정유정 (경기도보건환경연구원 안양농수산물검사소) ;
  • 류지은 (경기도보건환경연구원 안양농수산물검사소) ;
  • 최종철 (경기도보건환경연구원 안양농수산물검사소) ;
  • 채경석 (경기도보건환경연구원 안양농수산물검사소) ;
  • 이진희 (경기도보건환경연구원 안양농수산물검사소) ;
  • 도영숙 (경기도보건환경연구원 안양농수산물검사소) ;
  • 박용배 (경기도보건환경연구원 안양농수산물검사소) ;
  • 윤미혜 (경기도보건환경연구원 안양농수산물검사소)
  • Received : 2019.07.01
  • Accepted : 2019.08.29
  • Published : 2019.10.30
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Abstract

In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at $40^{\circ}C$ using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a $0.45{\mu}m$ membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.

본 연구에서는 chlorogenic acid 이성질체 9종과 arbutin을 분석하기 위하여, 전처리 과정과 UHPLC-MS/MS 동시분석 조건을 확립하였다. UHPLC-MS/MS에서 C18 column을 사용하여 15분 동안 분석할 수 있는 용매 조성을 만들고, 10가지 물질에 대한 정량, 정성 이온을 선택하여 negative 모드에서 분석하는 동시분석법을 확립하였고 과일류를 대상으로 추출, 진탕추출, 초음파 추출, 원심분리, 농축 등의 과정을 거치는 전처리법을 개발하였다. 기기분석과 전처리법에 대한 유효성은 특이성, 직선성, 정확성, 정밀성 그리고 검출한계 및 정량한계 등을 통하여 확인하였다. 회수율은 48.1-120.3%, intraday precision(RSD)는 0.4-7.9%, interday precision (RSD)는 0.0-7.2%로 확인되었다. 따라서, 본 연구에서 확립한 동시분석방법은 과일류 중 chlorogenic acid와 arbutin을 효과적으로 분석하는데 유용할 것으로 사료된다.

Keywords

References

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