Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Anaesthetic Efficacy and Physiological Response of Clove Oil and Lidocaine-HCl on River Puffer, Takifugu obscurus and Tiger Puffer, T. rubripes

  • Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime & Ocean University)
  • Received : 2018.12.29
  • Accepted : 2019.02.16
  • Published : 2019.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of the anaesthetic agents, clove oil and mixture of clove oil with lidocaine-HCl were evaluated on river puffer, Takifugu obscurus and tiger puffer, T. rubripes. Anaesthesia times of clove oil were affected by water temperature ($20^{\circ}C$, $24^{\circ}C$, and $28^{\circ}C$) and salinity (10, 20, and 30 ppt). Anaesthesia times of mixed samples were significantly similar with regard to exposure and recovery times, and all samples satisfied anaesthesia criteria (exposure time within 3 min and recovery time within 5 min) under the various temperatures and salinities, and the lowest to highest concentration of anaesthetics (p<0.05). Both species river puffer and tiger puffer had short exposure time with a high anaesthesia dose, high temperature ($28^{\circ}C$) and intermediate salinity (20 ppt), and were highly affected by temperature and salinity (p<0.05). The mixed anaesthetics had rapid exposure times and long recovery times in contrast to the effects of clove oil. Cortisol concentrations under the conditions of various clove oil dosages, salinity, and temperature for both species increased until 12 hrs after recovery from anaesthesia (p<0.05). After 12 hrs, cortisol concentrations decreased until after 48 hrs (p<0.05). During the simulated transportation of both species, control and sedated clove oil groups (5 ppm) were measured for water parameters, dissolved oxygen (DO), $CO_2$, respiratory frequency, $NH_4{^+}$, and pH for 6 hrs in 1 hr intervals. Water parameters of sedated groups and controls were significantly different after 2 hrs (p<0.05).

Keywords

References

  1. Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  2. Bell GR (1987) An outline of anesthetics and anesthesia for salmonids, a guide for fish culturists in British Columbia. Can Technic Rep Fish Aquat Sci 1534:16.
  3. Cho GK, Heath DD (2000) Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum). Aquacult Res 31:537-546. https://doi.org/10.1046/j.1365-2109.2000.00478.x
  4. Cooke SJ, Suski CD, Ostrand KG, Tufts BL, Walh DH (2004) Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides. Aquaculture 239:509-529. https://doi.org/10.1016/j.aquaculture.2004.06.028
  5. Donaldson EM (1981) The pituitary-interrenal axis as an indicator of stress in fish. In: Pickering AD (ed), Stress and Fish, Academic Press, London, pp 11-47.
  6. Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  7. Ghazilou A, Hasankandi HS, Chenary F, Nateghi A, Haghi N, Sahraeean MR (2010) The anesthetic efficiency of clove oil in Caspian salmon, Salmo trutta Caspius K., Smolts in dosage-salinity-pH linked approach. J World Aquacult Soc 41:655-660. https://doi.org/10.1111/j.1749-7345.2010.00407.x
  8. Gil HW, Ko MG, Lee TH, Park IS, Kim DS (2016) Anesthetic effect and physiological response in olive flounder (Paralichthys olivaceus) to clove oil in a simulated transport experiment. Dev Reprod 20:255-266. https://doi.org/10.12717/DR.2016.20.3.255
  9. Kang BS, Go HB, Kim SJ, Na OS, Lee CH, Kim SY, Lee J, Lee YD (2005) External symptoms of tiger puffer, Takifugu rubripes infected with scuticociliates and distribution of the scuticociliates in the skin, gill and blood vessel. J Fish Pathol 18:29-37.
  10. Kikuchi K, Furuta T, Ishizuka H, Yanagawa T (2007) Growth of tiger puffer, Takifugu rubripes, at different salinities. J World Aquacult Soc 38:427-434. https://doi.org/10.1111/j.1749-7345.2007.00114.x
  11. Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: Dynamics, mechanisms of action, and metabolic regulation. Rev Fish Bio Fisher 9:211-268. https://doi.org/10.1023/A:1008924418720
  12. Munday PL, Wilson SK (1997) Comparative efficacy of clove oil and other chemicals in anaesthetization of Pomacentrus amboinensis, a coral reef fish. J Fish Biol 51:931-938. https://doi.org/10.1006/jfbi.1997.0498
  13. Park IS, Gil HW, Lee TH, Nam YK, Lim SG, Kim DS (2017a) Effects of clove oil and lidocaine-HCl anesthesia on water parameter during simulated transportation in the marine medaka, Oryzias dancena. Dev Reprod 21:19-33. https://doi.org/10.12717/DR.2017.21.1.019
  14. Park IS, Jo JH, Lee SJ, Kim YA, Park KE, Hur JW, Yoo JS, Song YC (2003) Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate and MS-222 on the greenling (Hexagrammos otakii). J Korean Fish Soc 36:449-453.
  15. Park IS, Kim JH, Jung JB, Im JH (1998a) Effect of lidocaine as an anaesthetic on Rhynchocypris oxycephalus and R. steindachneri. J Aquacult 11:59-66.
  16. Park IS, Lim CH, Choi MS (1998b) The evaluation of lidocaine-hydrochloride as anaesthetic for the transportation of Rhynchocypris steindachneri. J Korean Fish Soc 31:785-790.
  17. Park IS, Lim SY, Lee TH, Gil HW, Yoo GY (2017b) Various characteristics of hybrid between river puffer, Takifugu obscurus and tiger puffer, T. rubripes, and their hybrid triploid. Dev Repord 21:181-191. https://doi.org/10.12717/DR.2017.21.2.181
  18. Park IS, Park MO, Hur JW, Kim DS, Chang YJ, Kim YJ, Park JY, Johnson SC (2009a) Anesthetic effects of lidocaine-hydrochloride on water parameters in simulated transport experiment of juvenile winter flounder, Pleuronectes americanus. Aquaculture 294:76-79. https://doi.org/10.1016/j.aquaculture.2009.05.011
  19. Park IS, Park SJ, Gil HW, Nam YK, Kim DS (2011) Anesthetic effects of clove oil and lidocaine-HCl on marine medaka (Oryzias dancena). Lab Anim (NY) 40:45-51. https://doi.org/10.1038/laban0211-45
  20. Park MO, Hur WJ, Im SY, Seol DW, Lee J, Park IS (2008b) Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquacult Res 39:877-884. https://doi.org/10.1111/j.1365-2109.2008.01941.x
  21. Park MO, Im SY, Seol DW, Park IS (2009b) Efficacy and physiological responses of rock bream, Oplegnathus fasciatus to anesthetization with clove oil. Aquaculture 287:427-430. https://doi.org/10.1016/j.aquaculture.2008.10.049
  22. Pirhonen J, Schreck CB (2003) Effects of anaesthesia with MS-222, clove oil and $CO_{2}$ on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture 220:507-514. https://doi.org/10.1016/S0044-8486(02)00624-5
  23. Ross LG, Ross B (2008) Anaesthetic and Sedative Techniques for Aquatic Animals. Oxford, UK, Blackwell Science, p 236.
  24. Schoettger RA, Steucke EW Jr (1970) Synergic mixtures of MS-222 and quinaldine as anesthetics for rainbow trout and Northern pike. Prog Fish-Cult 32:202-205. https://doi.org/10.1577/1548-8640(1970)32[202:SMOMAQ]2.0.CO;2
  25. Sneddon LU (2012) Clinical anesthesia and analgesia in fish. J Exotic Pet Med 21:32-43. https://doi.org/10.1053/j.jepm.2011.11.009
  26. Summerfelt RC, Smith LS (1990) Anesthesia, surgery, and related techniques. In: Schreck CB, Moyle PB (eds), Methods for Fish Biology. American Fisheries Society, Bethesda, MD, pp 213-272.
  27. Westerfield M (1993) The Zebrafish Book. Eugene, OR, University of Oregon Press, p 342.
  28. Woolsey J, Holcomb M, Ingermann RL (2004) Effect of temperature on clove oil anesthesia in steelhead fry. North Amer J Aquacult 66:35-41. https://doi.org/10.1577/A03-008
  29. Zahl IH, Kiessling A, Samuelsen OB, Hansen MK (2011) Anaesthesia of Atlantic halibut (Hippoglossus hippoglossus) effect of pre-anaesthetic sedation, and importance of body weight and water temperature. Aquacult Res 42:1235-1245. https://doi.org/10.1111/j.1365-2109.2010.02711.x