Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Medium and Soil Conditions on Propagation of Gametophyte and Sporophyte in Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike

진퍼리고사리의 전엽체 및 포자체 증식에 영향을 미치는 배지 조성과 배양토의 종류

  • Lee, Sang In (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Jang, Bo Kook (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University)
  • 이상인 (충북대학교 축산.원예.식품공학부 BK21플러스 생물건강소재산업화사업단) ;
  • 장보국 (충북대학교 축산.원예.식품공학부 BK21플러스 생물건강소재산업화사업단) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 BK21플러스 생물건강소재산업화사업단)
  • Received : 2019.03.16
  • Accepted : 2019.07.12
  • Published : 2019.08.31
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Abstract

This study was carried out to select the optimal condition for prothallus propagation and sporophyte formation of Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike and to provide basic data for mass production system. In the propagation, 300 mg of prothallus were inoculated in different kinds of medium [1/4, 1/2, Murashige and Skoog (MS), Knop], sucrose (0, 1, 2, 3, 4%), and cultured for 8 weeks. Sporophyte formation studies were carried out by inoculating blended prothallus into artificial soils. The soils were mixed with horticultural substrate, peatmoss, perlite, and decomposed granite at different ratios or only with the horticultural substrate. Then the prothallus was cultivated for 12 weeks to figure out the formation and growth of the sporophytes. The growth and development of prothalli were excellent on MS medium. According to medium components, prothallus growth was favorable in all treatments except 0% sucrose treatment and the highest in 2% sucrose. In the experiment of soil mixtures, sporophyte formation was the highest in the horticultural substrate:perlite 2:1 (v:v) and horticultural substrate:decomposed granite 2:1 (v:v) treatment, and the overall growth was good in the horticultural substrate:decomposed granite 2:1 (v:v) mixed soil.

본 연구는 진퍼리고사리의 전엽체 증식을 위한 배지를 선발하고, 포자체 증식을 위한 적정 배양토를 구명하여 대량생산 체계를 구축하고자 수행되었다. 전엽체 증식연구는 배지종류(1/4, 1/2, MS 배지, Knop 배지), sucrose (0, 1, 2, 3, 4%),의 농도를 달리한 배지에 전엽체 300 mg를 각각 접종하여 8주간 배양한 후 전엽체의 생육을 비교하였다. 포자체 형성 연구는 분쇄한 전엽체를 인공토양위에 접종하여 수행하였다. 인공토양은 원예상토, 펄라이트, 마사토의 비율을 다르게 조성하거나 원예상토 단용으로 사용했다. 그 후 접종한 전엽체를 12주간 배양한 다음 포자체 형성 및 생육을 조사하였다. 본 연구의 결과, 기내 전엽체는 2% sucrose를 첨가한 MS 배지에서 생체중과 생육이 가장 우수하였다. 포자체 형성을 위한 배양토 조성 실험에서는 원예상토:펄라이트 2:1(v:v)와 원예상토:마사토 2:1(v:v) 처리구에서 포자체의 형성이 가장 많았으며, 전반적인 생육은 원예상토:마사토 2:1(v:v)가 우수하였다.

Keywords

References

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