The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Bioactive Compounds, Anti-Oxidant Activities and Anti-Inflammatory Activities of Solvent Extracts from Centella asiatica Cultured in Chungju

충주산 병풀 용매추출물의 활성성분과 항산화 및 항염증 활성

  • Shin, Hyun-Young (Dept. of Integrated Biomedical and Life Science, Korea University) ;
  • Kim, Hoon (Skin Biotechnology Center, Kyung Hee University) ;
  • Jeong, Eun-Jin (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Kim, Jeung-Eun (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Lee, Kyeong-Haeng (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Bae, Yun-Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
  • 신현영 (고려대학교 의생명융합과학과) ;
  • 김훈 (경희대학교 피부생명공학센터) ;
  • 정은진 (한국교통대학교 식품영양학전공) ;
  • 김정은 (한국교통대학교 식품영양학전공) ;
  • 이경행 (한국교통대학교 식품영양학전공) ;
  • 배윤정 (한국교통대학교 식품영양학전공) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2020.11.06
  • Accepted : 2020.11.30
  • Published : 2020.12.31
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Abstract

Centella asiatica (CA) has been widely used as herbal plants. It is a valuable resources. The aim of the present study was to evaluate physiological activities of solvent extracts from CA cultivated in Chungju, Korea (Good tiger care). After preparing water (cold-water, CA-WE; hot-water, CA-HWE) and EtOH extracts (50% EtOH, CA-50E; 70% EtOH, CA-70E), their total polyphenol, flavonoid, and triterpenoid contents, and anti-oxidant activities, and anti-inflammatory activities were examined and compared. CA-70E showed the most potent anti-oxidant activity based on ABTS radical scavenging activity and reducing ability assays, while CA-50E and CA-70E showed the highest DPPH radical scavenging activity. Among major triterpenoid glycosides present in CA, madecassoside and asiaticoside contents were found to be the highest in CA-70E, and madecassic acid and asiatic acid were the highest in CA-50E. In LPS-stimulated RAW 264.7 cells, CA-70E showed the highest TNF-α inhibitory activity, although CA-50E and CA-70E similarly inhibited nitric oxide production. Ethanol extracts significantly inhibited IL-6 and IL-8 production more than water extracts using TNF-α/IFN-γ-stimulated HaCaT cells, indicating its better inhibitory against skin inflammation. Therefore, Chungju-CA EtOH extract, especially 70% EtOH extract, has high physiologically active ingredients and potent anti-oxidant and anti-inflammatory activities, suggesting its industrial application as a functional material.

Keywords

References

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