The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparison of Ingredient Quantities and Anti-Fatigue Effects of Jakyakgamcho-Tang according to Extraction Solvent

작약감초탕의 추출용매에 따른 성분 분석 및 항피로 효능 비교

  • Sung, Yoon-Young (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Yuk, Heung Joo (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Dong-Seon (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 성윤영 (한국한의학연구원 한약연구부) ;
  • 육흥주 (한국한의학연구원 한약연구부) ;
  • 김동선 (한국한의학연구원 한약연구부)
  • Received : 2020.02.25
  • Accepted : 2020.03.25
  • Published : 2020.03.30
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Abstract

Objectives : Jakyakgamcho-tang has been used as analgesic and spasmolytic for muscle pain. It has reported to anti-diabetes, anti-inflammation, and neuro-protective effects. A clinical study of muscle fatigue and pain improvement effect of Jakyakgamcho-tang has been reported with increasing frequency of clinical use. However, the anti-fatigue effect of Jakyakgamcho-tang in animal model has not been studied. In this study, we compared anti-physical fatigue effects of water and 30% ethanol extract of Jakyakgamcho-tang. Methods : The amounts of components contained in water and 30% ethanol extract of Jakyakgamcho-tang were compared by ultra-performance liquid chromatography. Anti-physical fatigue effects were evaluated using weight-loaded forced swimming test. We also investigated the effects of Jakyakgamcho-tang on the change of fatigue parameters by blood biochemical analysis. Results : The relative amount of components of Jakyakgamcho-tang were about 19-53% higher in the 30% ethanol extract than in the water extract. In the swimming test, 30% ethanol extract showed a significantly greater anti-fatigue effects than conventional water extract of Jakyakgamcho-tang. The 30% ethanol extract of Jakyakgamcho-tang improved the exhausted swimming time (54%), and serum lactate dehydrogenase (48%) and lactic acid (60%) levels compared with water extract of Jakyakgamcho-tang. Conclusions : These results showed that differences in the amounts of components by different extraction were associated with differences of anti-fatigue effect of Jakyakgamcho-tang. Thus, the 30% ethanol extraction method could be applied to dose-reducing formulations of traditional herbal medicines.

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